Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   TO73_RS02685 Genome accession   NZ_CP010822
Coordinates   461238..463493 (-) Length   751 a.a.
NCBI ID   WP_003044363.1    Uniprot ID   -
Organism   Thermus aquaticus Y51MC23     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 456238..468493
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TO73_RS02660 (TO73_0549) - 456533..456907 (-) 375 WP_003044347.1 4'-phosphopantetheinyl transferase superfamily protein -
  TO73_RS02665 (TO73_0550) rsmF 456979..458346 (+) 1368 WP_003044350.1 16S rRNA (cytosine(1407)-C(5))-methyltransferase RsmF -
  TO73_RS02670 (TO73_0551) - 458434..459483 (-) 1050 WP_003044354.1 3-dehydroquinate synthase family protein -
  TO73_RS02675 (TO73_0552) - 459461..460015 (-) 555 WP_003044357.1 shikimate kinase -
  TO73_RS02680 (TO73_0553) aroC 460028..461179 (-) 1152 WP_003044360.1 chorismate synthase -
  TO73_RS02685 (TO73_0554) pilQ 461238..463493 (-) 2256 WP_003044363.1 secretin N-terminal domain-containing protein Machinery gene
  TO73_RS02690 (TO73_0555) pilW 463505..464434 (-) 930 WP_003044366.1 hypothetical protein Machinery gene
  TO73_RS02695 (TO73_0556) pilO 464431..465030 (-) 600 WP_003044369.1 type 4a pilus biogenesis protein PilO Machinery gene
  TO73_RS02700 (TO73_0557) pilN 465020..465643 (-) 624 WP_003044372.1 competence protein Machinery gene
  TO73_RS02705 (TO73_0558) pilM 465636..466769 (-) 1134 WP_003044375.1 type IV pilus assembly protein PilM Machinery gene
  TO73_RS02710 (TO73_0559) - 467048..468052 (-) 1005 WP_003044377.1 homoisocitrate dehydrogenase -

Sequence

Protein


Download         Length: 751 a.a.        Molecular weight: 81354.78 Da        Isoelectric Point: 5.7744

>NTDB_id=140097 TO73_RS02685 WP_003044363.1 461238..463493(-) (pilQ) [Thermus aquaticus Y51MC23]
MKPLMLVLAALSVGALAKFPDEPRFQAKVDLKVSESQVKAGLTLPLDVVLEALAKSVGLQPLIYRAYDPSGDPAKAQPPL
PNVKLDFQGKPFREVWDLLFATYGAQFGLDYLFLPPDVVVVAPTQVITALVDAPSRTGAAERRPYIVGIPEIAYRRTETD
AQGQARTVVNIDGAKAWVQNDLLPFLSREAAGMGVNWIVVEEGGKLKAILSVLATPEQHARFSDLLKRAGIDFRPLPALA
EPKPRVERTYALTHATFPELLAFLQGQVPGAQVSVVPTDPKKAVVLATEEDHARLAELLKAADLPKTVRRVYALQNLTFE
EAKARLEPLLQRELKGARLEAIPGNPKALLLEATEADQALFAELLKAADVVPQAPPPQAEATVRKLYPLRFADAEKVAPF
LAREVPGIVVQTVPGQPVLSVRGTAKQLSEVESLLAQIDRAPEQGPPIFQRTFQLSNAKAKDLAQVLQEALKAKAAPPAQ
GQGQSGNPTPTATVVADERTNTLIVTGTAEDLALVEGLIPRLDQAVPQVNLRVRIQEVQSNLTRSLGLKWNTIAGGNVVA
SILDSGLSLIFDSTRSLAALNIVATLDALQRQGLSRALRDVNQTVLSNQTARLQSGETFLIRRVVENRVERVPFDIGIIV
EVTPQITADGQILLNIRAEVSGNVQRNPVDGDVDRFTKQVVTTTLRVRDGQTVVLGGLTSQENTQTVQGVPILMDIPLIG
ELFKQRTGETTDRELLVVITADILKETAERP

Nucleotide


Download         Length: 2256 bp        

>NTDB_id=140097 TO73_RS02685 WP_003044363.1 461238..463493(-) (pilQ) [Thermus aquaticus Y51MC23]
ATGAAGCCCCTGATGCTGGTTCTCGCCGCCTTGAGCGTGGGCGCTCTGGCCAAGTTTCCCGACGAGCCCCGCTTCCAGGC
GAAGGTGGACCTGAAGGTCTCGGAAAGCCAGGTCAAGGCCGGGCTCACCCTGCCCCTGGACGTGGTCCTCGAGGCCCTGG
CCAAGAGCGTGGGCCTCCAGCCCCTCATCTACCGGGCCTACGACCCCTCCGGCGACCCCGCCAAGGCCCAGCCCCCCCTG
CCCAACGTGAAGCTGGACTTCCAGGGGAAGCCCTTCCGGGAGGTGTGGGACCTCCTCTTCGCCACCTACGGGGCCCAGTT
CGGGCTGGACTACCTCTTCCTGCCCCCGGACGTGGTGGTGGTGGCCCCCACCCAGGTGATCACCGCCCTGGTGGACGCCC
CAAGCCGCACCGGGGCGGCGGAGAGGCGGCCCTACATCGTGGGCATCCCCGAGATCGCCTACCGGCGCACCGAAACCGAC
GCCCAGGGACAGGCCCGCACCGTGGTCAACATAGACGGGGCCAAGGCCTGGGTGCAGAACGACCTCCTGCCCTTCCTCTC
CCGGGAGGCGGCGGGGATGGGCGTGAACTGGATCGTGGTGGAGGAGGGGGGCAAGCTCAAGGCCATCCTCTCCGTCCTGG
CCACCCCGGAGCAGCACGCCCGCTTCTCCGACCTCCTGAAGCGAGCGGGGATTGACTTCCGCCCCCTTCCCGCCCTGGCC
GAGCCCAAGCCGCGGGTGGAAAGGACCTACGCCCTCACCCACGCCACCTTCCCCGAGCTTCTCGCCTTCCTCCAGGGCCA
GGTGCCCGGGGCCCAGGTGAGCGTGGTGCCCACCGACCCCAAAAAGGCGGTGGTGCTGGCCACCGAGGAGGACCACGCCC
GCCTGGCCGAGCTCCTGAAGGCCGCCGACCTCCCCAAGACCGTCCGCCGGGTCTACGCCCTGCAAAACCTCACCTTTGAG
GAGGCCAAAGCCCGGCTAGAACCCCTCCTGCAAAGGGAGCTCAAGGGGGCCCGCCTCGAGGCCATCCCCGGCAACCCCAA
AGCCCTCCTCCTGGAGGCCACCGAGGCCGACCAGGCCCTCTTCGCCGAGCTCCTCAAGGCCGCCGACGTAGTGCCCCAGG
CACCCCCGCCCCAGGCGGAGGCCACGGTGCGCAAGCTCTACCCCTTGCGCTTCGCCGACGCCGAGAAGGTGGCCCCCTTC
TTGGCCCGGGAAGTGCCGGGCATCGTGGTCCAGACCGTTCCCGGCCAGCCCGTCCTCTCGGTGCGGGGCACGGCGAAGCA
GCTTTCCGAGGTGGAAAGCCTCCTGGCCCAGATTGACCGGGCCCCCGAGCAGGGCCCTCCCATCTTTCAGCGGACCTTCC
AGCTCTCCAACGCCAAGGCCAAGGACCTGGCCCAGGTCCTCCAAGAGGCCCTGAAGGCCAAAGCCGCGCCTCCGGCCCAG
GGGCAGGGCCAGAGCGGGAACCCCACCCCCACGGCCACGGTGGTGGCCGACGAGCGCACCAACACCCTGATCGTCACCGG
GACCGCCGAGGACCTGGCCCTGGTGGAGGGGCTCATCCCCAGGCTGGACCAGGCGGTGCCCCAGGTGAACCTGAGGGTGC
GGATCCAGGAGGTCCAGTCCAACCTGACCCGGAGCCTGGGCCTCAAGTGGAACACCATCGCCGGGGGGAACGTGGTGGCC
AGCATCCTGGACTCGGGGCTTTCCCTCATCTTTGACTCCACCCGGAGCCTGGCCGCCTTGAACATCGTGGCCACCCTGGA
TGCCCTCCAAAGGCAGGGGCTCTCCCGGGCCCTTAGGGACGTGAACCAGACCGTCCTCAGCAACCAGACCGCCCGGCTCC
AGTCCGGGGAGACCTTCCTCATCCGCCGGGTGGTGGAGAACCGGGTGGAGCGGGTGCCCTTTGACATCGGCATCATCGTG
GAGGTGACCCCGCAGATCACCGCCGACGGGCAGATCCTCCTGAACATCCGGGCGGAGGTCTCCGGCAACGTGCAGAGGAA
CCCCGTGGACGGGGACGTGGACCGCTTCACCAAGCAGGTGGTGACCACCACCTTAAGGGTCAGGGACGGCCAAACCGTGG
TCCTGGGGGGCCTCACCTCCCAGGAGAACACCCAGACCGTCCAGGGCGTGCCCATCCTCATGGACATCCCCCTGATCGGG
GAGCTCTTCAAGCAGCGCACCGGCGAGACCACGGACCGGGAGCTCCTCGTGGTCATCACCGCCGACATCCTGAAGGAGAC
GGCAGAAAGGCCCTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(451-528)

Bacterial type II/III secretion system short domain (Secretin_N)

(386-444)

Bacterial type II and III secretion system protein (Secretin)

(588-745)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Thermus thermophilus HB27

83.624

99.201

0.83

Multiple sequence alignment