Detailed information NTG0139208

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: EG09_RS19410
• Genome accession: NZ_CP010555
• Coordinates: 4247432..4248580 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain FRD1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4242432..4253580

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EG09_RS19385 (EG09_19385)	-	4243583..4243879 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
EG09_RS19390 (EG09_19390)	-	4243876..4244469 (-)	594	WP_003084544.1	YggT family protein	-
EG09_RS19395 (EG09_19395)	proC	4244480..4245301 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
EG09_RS19400 (EG09_19400)	-	4245313..4246005 (-)	693	WP_015649764.1	YggS family pyridoxal phosphate-dependent enzyme	-
EG09_RS19405 (EG09_19405)	pilT	4246220..4247254 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
EG09_RS19410 (EG09_19410)	pilU	4247432..4248580 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
EG09_RS19415 (EG09_19415)	czcD	4248588..4249487 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
EG09_RS19420 (EG09_19420)	-	4249641..4250045 (+)	405	WP_003084560.1	NINE protein	-
EG09_RS19425 (EG09_19425)	-	4250288..4251661 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
EG09_RS19430 (EG09_19430)	-	4251757..4252841 (+)	1085	Protein_3911	trans-sulfuration enzyme family protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=139208 EG09_RS19410 WP_003100959.1 4247432..4248580(+) (pilU) [Pseudomonas aeruginosa strain FRD1]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=139208 EG09_RS19410 WP_003100959.1 4247432..4248580(+) (pilU) [Pseudomonas aeruginosa strain FRD1]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGTCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382