Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MJ49_RS14425 Genome accession   NZ_CP013025
Coordinates   2734838..2736319 (-) Length   493 a.a.
NCBI ID   WP_025491952.1    Uniprot ID   -
Organism   Escherichia coli strain 2009C-3133     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2729838..2741319
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MJ49_RS14395 (MJ49_13655) gspK 2730751..2731734 (-) 984 WP_001058528.1 type II secretion system minor pseudopilin GspK -
  MJ49_RS14400 (MJ49_13660) gspJ 2731727..2732314 (-) 588 WP_000610629.1 type II secretion system minor pseudopilin GspJ -
  MJ49_RS14405 (MJ49_13665) gspI 2732307..2732684 (-) 378 WP_001041738.1 type II secretion system minor pseudopilin GspI -
  MJ49_RS14410 (MJ49_13670) gspH 2732681..2733190 (-) 510 WP_001076031.1 type II secretion system minor pseudopilin GspH -
  MJ49_RS30880 (MJ49_13675) gspG 2733198..2733635 (-) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -
  MJ49_RS14420 (MJ49_13680) gspF 2733645..2734841 (-) 1197 WP_001109559.1 type II secretion system inner membrane protein GspF -
  MJ49_RS14425 (MJ49_13685) pilB 2734838..2736319 (-) 1482 WP_025491952.1 type II secretion system ATPase GspE Machinery gene
  MJ49_RS14430 (MJ49_13690) gspD 2736329..2738281 (-) 1953 WP_001562179.1 type II secretion system secretin GspD -
  MJ49_RS14435 (MJ49_13695) gspC 2738265..2739080 (-) 816 WP_001142702.1 type II secretion system protein GspC -
  MJ49_RS14440 (MJ49_13700) gspA 2739260..2740729 (+) 1470 WP_000107581.1 type II secretion system protein GspA -
  MJ49_RS14445 (MJ49_13705) gspB 2740731..2741150 (+) 420 WP_000461444.1 putative general secretion pathway protein GspB -

Sequence

Protein


Download         Length: 493 a.a.        Molecular weight: 54579.51 Da        Isoelectric Point: 6.2406

>NTDB_id=138376 MJ49_RS14425 WP_025491952.1 2734838..2736319(-) (pilB) [Escherichia coli strain 2009C-3133]
MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQVGQCQTMTSLSQADFEARLEAVFHQNTGESQQIAQDI
DQSVDLLSLSEEMPANEDLLNEDSAAPVIRLINAILSEAIKETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALL
ISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQLSLNNLGMTAADKQDLENLIQL
PHGIILVTGPTGSGKSTTLYAILSALNTPGRNILTVEDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQAPDVVMVGEI
RDTETAQIAVQASLTGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSLAGIIAQRLVRRLCPQCRQFTPVSPQQAQMFK
YHQLAVTTIGTPVGCPHCHQSGYQGRMAIHEMMVVTPELRAAIHENVDEQALERLVRQQHNALIKNGLQKVISGDTSWDE
VMRVASATLENEA

Nucleotide


Download         Length: 1482 bp        

>NTDB_id=138376 MJ49_RS14425 WP_025491952.1 2734838..2736319(-) (pilB) [Escherichia coli strain 2009C-3133]
ATGAGAATTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTAT
TTATCTCGCCGATACGCCATTCGAACGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGT
CACAGGCTGATTTTGAAGCCCGGCTGGAAGCGGTGTTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATC
GATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACC
GGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCCGATATCCACATTGAAACCTATGAAA
AAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTT
ATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGACGTATTAGTTTGCGTAT
CGGGCGACGTAACATAGATGTCCGCGTATCCACACTGCCGTCCATCTATGGTGAACGCGCCGTGCTCCGTCTGCTGGATA
AAAACAGCCTCCAGCTTTCATTGAACAACCTGGGGATGACGGCAGCGGATAAACAGGATTTAGAAAATCTCATCCAGCTT
CCACACGGTATTATCCTGGTGACCGGACCGACAGGCTCCGGTAAAAGCACCACGCTCTACGCCATCCTTTCGGCGCTGAA
TACTCCCGGCCGCAATATTCTGACGGTAGAAGATCCCGTGGAATATGAGCTGGAAGGCATTGGGCAAACGCAGGTGAATA
CCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGCCCCGGATGTCGTCATGGTGGGGGAAATT
CGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCCACACTAACAG
TGCATCAGGCGCAGTGACCCGGCTCCGCGACATGGGCGTCGAATCATTCCTGCTTTCGTCTTCCCTGGCAGGGATTATCG
CGCAACGTCTGGTTCGTCGCCTGTGTCCGCAATGCCGACAATTCACGCCCGTATCACCCCAACAAGCGCAGATGTTTAAA
TATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCTGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCAT
GGCGATCCACGAAATGATGGTGGTGACGCCGGAATTACGGGCCGCTATTCATGAAAATGTGGATGAACAAGCACTGGAGC
GACTGGTCCGGCAACAACACAATGCCTTAATCAAAAATGGCCTGCAAAAAGTGATAAGCGGTGACACCTCCTGGGATGAG
GTTATGCGCGTCGCCAGTGCCACGCTGGAGAACGAAGCATGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(114-380)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

46.453

88.641

0.412
  pilB Acinetobacter baumannii D1279779

45.578

89.452

0.408
  pilB Vibrio cholerae strain A1552

48.201

84.584

0.408
  pilB Legionella pneumophila strain ERS1305867

41.164

97.566

0.402
  pilB Vibrio parahaemolyticus RIMD 2210633

46.118

86.207

0.398
  pilB/pilB1 Synechocystis sp. PCC 6803

40.987

94.523

0.387
  pilB Acinetobacter baylyi ADP1

48.338

79.31

0.383