Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   SB85_RS17630 Genome accession   NZ_CP010409
Coordinates   4348242..4349300 (+) Length   352 a.a.
NCBI ID   WP_043094893.1    Uniprot ID   -
Organism   Xanthomonas sacchari strain R1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4343242..4354300
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SB85_RS17615 (SB85_17685) - 4343339..4344628 (+) 1290 WP_026143410.1 citrate synthase -
  SB85_RS17620 (SB85_17690) - 4344800..4345444 (-) 645 WP_017909856.1 hypothetical protein -
  SB85_RS17625 (SB85_17695) - 4345597..4348023 (-) 2427 WP_043094892.1 penicillin-binding protein 1A -
  SB85_RS17630 (SB85_17700) pilM 4348242..4349300 (+) 1059 WP_043094893.1 pilus assembly protein PilM Machinery gene
  SB85_RS17635 (SB85_17705) - 4349300..4350019 (+) 720 WP_043094894.1 PilN domain-containing protein -
  SB85_RS17640 (SB85_17710) - 4350016..4350690 (+) 675 WP_043094895.1 type 4a pilus biogenesis protein PilO -
  SB85_RS17645 (SB85_17715) - 4350687..4351226 (+) 540 WP_043094897.1 pilus assembly protein PilP -
  SB85_RS17650 (SB85_17720) - 4351247..4353142 (+) 1896 WP_043094898.1 type IV pilus secretin PilQ family protein -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 37709.33 Da        Isoelectric Point: 4.4460

>NTDB_id=136948 SB85_RS17630 WP_043094893.1 4348242..4349300(+) (pilM) [Xanthomonas sacchari strain R1]
MGLIPKSQQPLIGIDISSTAVKLLQLSRNGNRFRVDHYAVEPLPPNAVVEKNIVEVEAVGEAIRRAVTRSGTRAKHAAAA
VAGSAVITKVIPMPAELDENELEAQVELEAVNYIPYPIDEVNLDFEVLGVIPNNPEMVQVLLAASRSENVELRQSALELG
GLVAKVMDVEAFAVENAFALVASELPVAADGIVALVDIGATMTTLNVLRGGRSLYSREQVFGGKQLTDEVMRRYGLTYEE
AGMAKRQGGLPESYEVEVLEPFKEATVQQISRLLQFFYAGSEFNRVDHIVLAGGCAALAGLPEMVEEQLGVATVVANPLA
QMTLGPKVQAHALAQDAPALMIATGLALRSFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=136948 SB85_RS17630 WP_043094893.1 4348242..4349300(+) (pilM) [Xanthomonas sacchari strain R1]
GTGGGGCTTATCCCAAAGAGTCAGCAACCGCTGATCGGCATCGATATCAGCTCGACCGCAGTCAAGCTGCTGCAGCTCTC
GCGTAACGGCAACCGTTTCCGCGTCGACCACTACGCCGTCGAACCCTTGCCGCCCAATGCCGTGGTCGAAAAGAACATCG
TCGAGGTCGAGGCGGTGGGCGAGGCGATCCGCCGCGCCGTGACCCGTTCCGGTACCCGTGCCAAGCATGCCGCCGCCGCC
GTGGCCGGTTCGGCGGTGATCACCAAGGTGATCCCGATGCCGGCCGAACTGGACGAGAACGAGCTGGAAGCGCAGGTCGA
GCTGGAGGCGGTGAACTACATCCCGTACCCGATCGACGAAGTGAACCTGGATTTCGAGGTGCTGGGCGTCATCCCCAACA
ACCCGGAGATGGTGCAGGTGCTGCTGGCCGCCTCGCGCTCGGAGAACGTGGAGCTGCGCCAGTCGGCGCTGGAGCTGGGC
GGCCTGGTGGCCAAGGTGATGGACGTGGAGGCCTTCGCGGTCGAGAACGCCTTCGCCCTGGTGGCCAGCGAACTGCCGGT
GGCCGCCGACGGCATCGTCGCGCTGGTCGACATCGGCGCCACCATGACCACGCTCAATGTCCTGCGTGGCGGGCGCAGCC
TGTACAGCCGCGAGCAGGTGTTCGGCGGCAAGCAGCTGACCGACGAAGTGATGCGCCGCTACGGCCTGACCTACGAGGAA
GCCGGCATGGCCAAGCGCCAGGGCGGGCTGCCGGAGAGCTACGAGGTCGAGGTGCTGGAACCGTTCAAGGAAGCCACGGT
GCAACAGATCAGCCGCCTGCTGCAGTTCTTCTACGCCGGCAGCGAGTTCAACCGGGTCGACCACATCGTCCTGGCCGGCG
GCTGCGCCGCCCTGGCCGGGTTGCCGGAGATGGTGGAGGAGCAACTGGGCGTGGCCACGGTGGTCGCCAACCCGTTGGCG
CAGATGACCCTGGGGCCGAAGGTCCAGGCGCATGCGCTGGCCCAGGACGCGCCGGCGCTGATGATCGCCACCGGCCTGGC
GCTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

51.136

100

0.511
  comM Acinetobacter nosocomialis M2

50.852

100

0.509
  comM Acinetobacter baylyi ADP1

49.432

100

0.494
  pilM Legionella pneumophila strain ERS1305867

47.429

99.432

0.472

Multiple sequence alignment