Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   AO353_RS03580 Genome accession   NZ_CP012830
Coordinates   764872..765933 (-) Length   353 a.a.
NCBI ID   WP_054593713.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain FW300-N2E3     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 759872..770933
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AO353_RS03550 (AO353_03550) pal 760177..760674 (-) 498 WP_054593710.1 peptidoglycan-associated lipoprotein Pal -
  AO353_RS03555 (AO353_03555) tolB 760727..762007 (-) 1281 WP_173859947.1 Tol-Pal system beta propeller repeat protein TolB -
  AO353_RS03560 (AO353_03560) tolA 762025..763104 (-) 1080 WP_054593712.1 cell envelope integrity protein TolA -
  AO353_RS03565 (AO353_03565) tolR 763104..763556 (-) 453 WP_162174768.1 protein TolR -
  AO353_RS03570 (AO353_03570) tolQ 763580..764275 (-) 696 WP_019693256.1 protein TolQ -
  AO353_RS03575 (AO353_03575) ybgC 764265..764732 (-) 468 WP_019693257.1 tol-pal system-associated acyl-CoA thioesterase -
  AO353_RS03580 (AO353_03580) ruvB 764872..765933 (-) 1062 WP_054593713.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  AO353_RS03585 (AO353_03585) ruvA 765934..766542 (-) 609 WP_054593714.1 Holliday junction branch migration protein RuvA -
  AO353_RS03590 (AO353_03590) ruvC 766661..767185 (-) 525 WP_019693260.1 crossover junction endodeoxyribonuclease RuvC -
  AO353_RS03595 (AO353_03595) - 767389..768135 (-) 747 WP_054593715.1 YebC/PmpR family DNA-binding transcriptional regulator -
  AO353_RS03600 (AO353_03600) aspS 768265..770040 (-) 1776 WP_054593716.1 aspartate--tRNA ligase -
  AO353_RS03605 (AO353_03605) - 770149..770370 (-) 222 WP_010457489.1 FmdB family zinc ribbon protein -
  AO353_RS03610 (AO353_03610) - 770538..770885 (-) 348 WP_173859946.1 ribbon-helix-helix domain-containing protein -

Sequence

Protein


Download         Length: 353 a.a.        Molecular weight: 39155.92 Da        Isoelectric Point: 4.9785

>NTDB_id=136592 AO353_RS03580 WP_054593713.1 764872..765933(-) (ruvB) [Pseudomonas fluorescens strain FW300-N2E3]
MIEADRLIAATGGPRDREEVQDRAIRPVSLAEYIGQPTVREQMELFIQAARGRNESLDHTLIFGPPGLGKTTLANIIAQE
MGVSIKSTSGPVLERPGDLAALLTNLEPHDVLFIDEIHRLSPIVEEVLYPAMEDFQLDIMIGEGPAARSIKLDLPPFTLV
GATTRAGMLTNPLRDRFGIVQRLEFYSTADLATIVSRSANILGLPLDPEGAFEIARRARGTPRIANRLLRRVRDFAEVRA
KGHITKPIADLALNLLDVDERGFDHQDRRLLLTMIEKFDGGPVGVDSLAAAISEERHTIEDVLEPYLIQQGYIMRTPRGR
VVTRHAYLHFGLNIPSRLGEMPVPDEFLDAVDE

Nucleotide


Download         Length: 1062 bp        

>NTDB_id=136592 AO353_RS03580 WP_054593713.1 764872..765933(-) (ruvB) [Pseudomonas fluorescens strain FW300-N2E3]
GTGATTGAAGCTGATCGTCTGATCGCCGCCACGGGTGGCCCCCGTGACCGCGAAGAAGTCCAGGACCGGGCGATTCGTCC
CGTCAGCCTGGCCGAATACATCGGCCAGCCGACCGTTCGCGAGCAGATGGAGTTGTTCATCCAGGCTGCCCGTGGGCGCA
ACGAATCCCTCGATCACACGTTGATCTTCGGTCCGCCGGGGCTGGGTAAAACCACCTTGGCCAACATCATTGCCCAGGAA
ATGGGCGTGTCGATCAAAAGCACCTCGGGGCCGGTCCTCGAGCGTCCGGGTGACCTCGCGGCGTTGCTGACCAACCTCGA
ACCGCATGATGTGCTGTTCATTGACGAGATCCATCGGCTGTCGCCGATTGTCGAAGAAGTGCTGTACCCGGCCATGGAAG
ACTTCCAGCTCGACATCATGATCGGTGAAGGGCCGGCGGCGCGTTCGATCAAGCTCGACCTGCCACCGTTCACCCTGGTC
GGCGCCACCACCCGTGCCGGGATGCTGACCAACCCGTTGCGTGACCGCTTCGGCATCGTTCAGCGCCTGGAGTTCTATAG
CACGGCGGATCTGGCGACCATCGTCAGCCGTTCGGCGAACATCCTCGGCTTGCCGCTGGACCCGGAGGGCGCCTTTGAAA
TCGCCCGTCGGGCCCGTGGTACGCCGCGTATCGCCAACCGCTTGTTGCGCCGGGTGCGCGATTTTGCCGAAGTCCGGGCG
AAGGGGCATATCACTAAACCGATTGCCGATCTGGCGCTGAACCTGCTGGACGTCGATGAGCGTGGCTTCGATCACCAGGA
CCGGCGTCTGCTGCTGACCATGATCGAGAAGTTCGATGGCGGCCCGGTCGGGGTGGACAGTCTCGCGGCGGCGATCAGCG
AAGAGCGGCACACCATCGAGGATGTGCTGGAACCCTATCTGATTCAGCAGGGCTATATCATGCGGACCCCGCGTGGTCGG
GTCGTAACCCGGCATGCGTACTTGCATTTCGGTTTGAATATCCCGTCACGGTTAGGTGAAATGCCCGTGCCAGACGAGTT
TCTTGATGCAGTAGACGAATGA

Domains

Predicted by InterProScan.

RuvB C-terminal winged helix domain (WHD_RuvB)

(260-330)

RuvB AAA lid domain (AAA_lid_4)

(186-259)

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(25-183)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

61.329

93.768

0.575
  ruvB Streptococcus pneumoniae TIGR4

59.375

90.652

0.538
  ruvB Streptococcus pneumoniae R6

59.375

90.652

0.538
  ruvB Streptococcus pneumoniae D39

59.375

90.652

0.538
  ruvB Synechocystis sp. PCC 6803

55.937

90.652

0.507
  ruvB Helicobacter pylori 26695

53.894

90.935

0.49

Multiple sequence alignment