Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   RO07_RS21725 Genome accession   NZ_CP010310
Coordinates   5054892..5055410 (-) Length   172 a.a.
NCBI ID   WP_039405706.1    Uniprot ID   A0AAJ4ZEB1
Organism   Pandoraea pulmonicola strain DSM 16583     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5049892..5060410
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RO07_RS21705 (RO07_04850) - 5050038..5051429 (-) 1392 WP_039405701.1 hypothetical protein -
  RO07_RS21710 (RO07_04855) - 5051574..5051744 (-) 171 WP_039405704.1 rubredoxin -
  RO07_RS21715 (RO07_04860) thiD 5051966..5052808 (+) 843 WP_039413242.1 bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase -
  RO07_RS21720 (RO07_04865) - 5052930..5054729 (-) 1800 WP_147284594.1 O-antigen ligase family protein -
  RO07_RS21725 (RO07_04870) pilA 5054892..5055410 (-) 519 WP_039405706.1 pilin Machinery gene
  RO07_RS21730 (RO07_04875) - 5055670..5056398 (-) 729 WP_039405709.1 TerC family protein -
  RO07_RS21735 (RO07_04880) sucD 5056546..5057427 (-) 882 WP_039405712.1 succinate--CoA ligase subunit alpha -
  RO07_RS21740 (RO07_04885) sucC 5057512..5058678 (-) 1167 WP_039405715.1 ADP-forming succinate--CoA ligase subunit beta -
  RO07_RS21745 (RO07_04890) - 5058675..5059355 (-) 681 WP_039405717.1 DUF2889 domain-containing protein -
  RO07_RS21750 (RO07_04895) recX 5059665..5060177 (-) 513 WP_418303692.1 recombination regulator RecX -

Sequence

Protein


Download         Length: 172 a.a.        Molecular weight: 17435.87 Da        Isoelectric Point: 9.5567

>NTDB_id=136210 RO07_RS21725 WP_039405706.1 5054892..5055410(-) (pilA) [Pandoraea pulmonicola strain DSM 16583]
MRRFGHRTTGIAKSRGFTLIELMIVLAIIGVLVTAGVPYLQNYLVRARVVEGLGAAASAKALVSENAMHGAPFNSGWQAP
SATDNVSGVTIDSVSGNVTVAYTQRAGDGAIVLVPTSAGAQGRAEALTVGKAPEGQIVWTCYAKGREGAPGGATLSAKLA
PPECRGAGSGND

Nucleotide


Download         Length: 519 bp        

>NTDB_id=136210 RO07_RS21725 WP_039405706.1 5054892..5055410(-) (pilA) [Pandoraea pulmonicola strain DSM 16583]
ATGCGTCGTTTCGGCCACCGGACCACCGGTATCGCCAAGTCCCGGGGCTTCACCCTGATCGAGTTGATGATCGTGTTGGC
GATCATCGGCGTGCTGGTCACGGCAGGGGTACCCTATCTGCAGAATTATCTGGTGCGCGCCCGGGTCGTCGAAGGGCTGG
GGGCTGCCGCATCGGCCAAGGCGCTCGTGAGCGAGAACGCGATGCATGGCGCGCCGTTCAACAGCGGTTGGCAGGCGCCG
AGCGCCACGGACAACGTGAGCGGTGTGACCATCGACTCCGTGAGCGGCAACGTGACGGTGGCCTACACGCAACGCGCCGG
CGACGGCGCGATCGTGCTGGTGCCCACGAGTGCCGGCGCGCAAGGCCGCGCGGAAGCGCTCACGGTCGGCAAGGCGCCCG
AGGGCCAGATCGTGTGGACGTGCTACGCCAAGGGCCGCGAAGGTGCGCCCGGTGGCGCCACGTTGTCGGCCAAGCTGGCG
CCCCCGGAGTGCCGCGGTGCGGGATCTGGGAATGACTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(13-35)

Pilin (bacterial filament) (Pilin)

(45-165)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

47.429

100

0.483
  pilA2 Legionella pneumophila str. Paris

40.506

91.86

0.372
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

33.333

100

0.372
  pilA2 Legionella pneumophila strain ERS1305867

39.873

91.86

0.366

Multiple sequence alignment