Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   AM599_RS20550 Genome accession   NZ_CP012585
Coordinates   4457338..4458396 (+) Length   352 a.a.
NCBI ID   WP_003086123.1    Uniprot ID   Q02IC9
Organism   Pseudomonas aeruginosa strain PA_D1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4452338..4463396
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AM599_RS20525 (AM599_20525) - 4452598..4453068 (-) 471 WP_003086103.1 Dps family protein -
  AM599_RS20530 (AM599_20530) aspS 4453508..4455283 (+) 1776 WP_069376817.1 aspartate--tRNA ligase -
  AM599_RS20535 (AM599_20535) - 4455350..4456096 (+) 747 WP_003086107.1 YebC/PmpR family DNA-binding transcriptional regulator -
  AM599_RS20540 (AM599_20540) ruvC 4456181..4456705 (+) 525 WP_003112575.1 crossover junction endodeoxyribonuclease RuvC -
  AM599_RS20545 (AM599_20545) ruvA 4456722..4457327 (+) 606 WP_003086122.1 Holliday junction branch migration protein RuvA -
  AM599_RS20550 (AM599_20550) ruvB 4457338..4458396 (+) 1059 WP_003086123.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  AM599_RS20555 (AM599_20555) ybgC 4458449..4458895 (+) 447 WP_003086124.1 tol-pal system-associated acyl-CoA thioesterase -
  AM599_RS20560 (AM599_20560) tolQ 4458897..4459592 (+) 696 WP_003086125.1 protein TolQ -
  AM599_RS20565 (AM599_20565) tolR 4459615..4460055 (+) 441 WP_003086126.1 protein TolR -
  AM599_RS20570 (AM599_20570) tolA 4460058..4461101 (+) 1044 WP_003086127.1 cell envelope integrity protein TolA -
  AM599_RS20575 (AM599_20575) tolB 4461098..4462396 (+) 1299 WP_003086128.1 Tol-Pal system beta propeller repeat protein TolB -
  AM599_RS20580 (AM599_20580) pal 4462449..4462955 (+) 507 WP_003111417.1 peptidoglycan-associated lipoprotein Pal -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 38932.67 Da        Isoelectric Point: 5.1792

>NTDB_id=134635 AM599_RS20550 WP_003086123.1 4457338..4458396(+) (ruvB) [Pseudomonas aeruginosa strain PA_D1]
MIEPDRLISAVSGRERDEQLDRAIRPLKLADYIGQPSVREQMELFIHAARGRQEALDHTLIFGPPGLGKTTLANIIAQEM
GVSIKSTSGPVLERPGDLAALLTNLEAGDVLFVDEIHRLSPIVEEVLYPAMEDFQLDIMIGEGPAARSIKLDLPPFTLVG
ATTRAGMLTNPLRDRFGIVQRLEFYNVEDLATIVSRSAGILGLEIEPQGAAEIAKRARGTPRIANRLLRRVRDFAEVRGQ
GDITRVIADKALNLLDVDERGFDHLDRRLLLTMIDKFDGGPVGIDNLAAALSEERHTIEDVLEPYLIQQGYIMRTPRGRV
VTRHAYLHFGLNIPKRLGPGVTTDLFTSEDGN

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=134635 AM599_RS20550 WP_003086123.1 4457338..4458396(+) (ruvB) [Pseudomonas aeruginosa strain PA_D1]
ATGATCGAGCCTGATCGCCTCATATCCGCCGTTTCCGGTCGCGAACGCGATGAGCAGTTGGACCGCGCCATTCGCCCGCT
GAAGCTGGCCGACTACATCGGCCAGCCCAGCGTGCGCGAGCAGATGGAGCTGTTCATCCATGCCGCGCGCGGCCGCCAGG
AGGCGCTCGACCATACGCTGATCTTCGGTCCGCCGGGGCTGGGCAAGACGACCCTGGCGAATATCATCGCCCAGGAAATG
GGCGTTTCCATCAAGAGTACTTCCGGGCCGGTGCTCGAGCGTCCCGGCGATCTCGCCGCGTTGCTGACCAACCTGGAGGC
CGGCGACGTCCTGTTCGTCGACGAGATCCACCGTCTTTCGCCCATCGTCGAAGAAGTGCTGTATCCGGCCATGGAAGATT
TCCAGTTGGACATCATGATCGGCGAAGGGCCCGCGGCCCGCTCCATCAAGCTGGACCTGCCGCCGTTCACCCTGGTCGGC
GCGACCACTCGTGCCGGCATGCTGACCAATCCACTGCGCGATCGCTTCGGTATCGTCCAGCGCCTGGAGTTCTACAACGT
CGAGGACCTGGCGACCATCGTCAGTCGGTCGGCCGGCATTCTCGGCCTGGAGATCGAGCCGCAGGGCGCGGCGGAAATCG
CCAAGCGCGCCCGCGGTACGCCGCGAATCGCCAATCGCCTGCTGCGCCGGGTACGCGACTTCGCCGAGGTCCGGGGGCAG
GGCGACATTACCCGGGTGATCGCCGACAAGGCATTGAACCTGCTCGATGTCGACGAGCGTGGCTTTGACCACCTCGACCG
TCGTCTGTTGCTGACCATGATCGACAAGTTCGATGGCGGGCCTGTAGGAATCGACAACTTGGCTGCGGCGTTGAGCGAGG
AGCGGCACACGATCGAAGATGTACTGGAGCCCTATCTGATCCAGCAGGGCTACATCATGCGGACACCGCGGGGACGCGTG
GTAACACGCCATGCGTACCTGCACTTTGGCCTGAATATTCCGAAAAGGTTGGGGCCGGGGGTGACGACGGATCTGTTCAC
GTCTGAAGATGGTAATTAA

Domains

Predicted by InterProScan.

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-329)

RuvB AAA lid domain (AAA_lid_4)

(185-258)

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(24-182)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q02IC9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

61.027

94.034

0.574
  ruvB Streptococcus pneumoniae TIGR4

59.062

90.909

0.537
  ruvB Streptococcus pneumoniae R6

58.75

90.909

0.534
  ruvB Streptococcus pneumoniae D39

58.75

90.909

0.534
  ruvB Synechocystis sp. PCC 6803

57.143

91.477

0.523
  ruvB Helicobacter pylori 26695

55.449

88.636

0.491