Detailed information NTG0133824

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: AMR84_RS01115
• Genome accession: NZ_CP012503
• Coordinates: 190036..190497 (+)
• Length: 153 a.a.
• NCBI ID: WP_001878820.1
• Uniprot ID: -
• Organism: Streptococcus agalactiae strain NGBS357
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 185036..195497

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AMR84_RS01085 (AMR84_01085)	-	186651..187016 (+)	366	WP_000285373.1	DUF1033 family protein	-
AMR84_RS01090 (AMR84_01090)	comGA	187189..188160 (+)	972	WP_000250805.1	competence type IV pilus ATPase ComGA	Machinery gene
AMR84_RS01095 (AMR84_01095)	comGB	188054..189097 (+)	1044	WP_223295889.1	competence type IV pilus assembly protein ComGB	Machinery gene
AMR84_RS01100 (AMR84_01100)	comGC/cglC	189094..189423 (+)	330	WP_000793380.1	competence type IV pilus major pilin ComGC	Machinery gene
AMR84_RS01105 (AMR84_01105)	comGD	189398..189811 (+)	414	WP_000244261.1	competence type IV pilus minor pilin ComGD	Machinery gene
AMR84_RS01110 (AMR84_01110)	comGE	189783..190082 (+)	300	WP_000234804.1	competence type IV pilus minor pilin ComGE	Machinery gene
AMR84_RS01115 (AMR84_01115)	comGF	190036..190497 (+)	462	WP_001878820.1	competence type IV pilus minor pilin ComGF	Machinery gene
AMR84_RS01120 (AMR84_01120)	comGG	190475..190846 (+)	372	WP_000601104.1	competence type IV pilus minor pilin ComGG	Machinery gene
AMR84_RS01125 (AMR84_01125)	comGH	190961..191935 (+)	975	WP_001008574.1	class I SAM-dependent methyltransferase	Machinery gene
AMR84_RS01130 (AMR84_01130)	-	191967..193160 (+)	1194	WP_000047534.1	acetate kinase	-
AMR84_RS01135 (AMR84_01135)	-	193312..193518 (+)	207	WP_000798241.1	helix-turn-helix transcriptional regulator	-
AMR84_RS10915	-	193577..193714 (+)	138	WP_001865900.1	hypothetical protein	-
AMR84_RS01140 (AMR84_01140)	-	193755..194210 (+)	456	WP_000905673.1	hypothetical protein	-
AMR84_RS01145 (AMR84_01145)	-	194279..194944 (+)	666	WP_000008113.1	CPBP family intramembrane glutamic endopeptidase	-

Sequence

Protein

• Length: 153 a.a.
• Molecular weight: 17874.95 Da
• Isoelectric Point: 10.0299

>NTDB_id=133824 AMR84_RS01115 WP_001878820.1 190036..190497(+) (comGF) [Streptococcus agalactiae strain NGBS357]
MKIIRKFCTYQKLRVKAFTLLKCLVALVTITGALLVYQGLTKLLAQQIVVMSSSSQSEWVLLTQQLNAEFEGAHLEYLRQ
NKLYLRKQDKIVTFGKSNKDDFRKTGYDGRGYQPMVYGLDNCQMSQTKSMVKLVFYFKDGLKRTFYYDFKEET

Nucleotide

• Length: 462 bp

>NTDB_id=133824 AMR84_RS01115 WP_001878820.1 190036..190497(+) (comGF) [Streptococcus agalactiae strain NGBS357]
TTGAAGATAATAAGGAAATTTTGCACGTATCAAAAGTTAAGGGTTAAAGCATTTACGCTTTTAAAATGTTTGGTAGCATT
GGTTACAATCACAGGAGCTTTACTAGTTTATCAAGGACTGACAAAATTGTTGGCTCAACAGATAGTAGTGATGTCTTCTT
CCAGTCAGTCTGAATGGGTGTTATTAACTCAGCAACTAAATGCAGAATTTGAAGGCGCTCATCTGGAATATTTAAGACAG
AACAAACTTTATTTACGTAAGCAAGATAAGATTGTAACCTTTGGCAAATCTAATAAAGATGATTTCCGTAAGACAGGTTA
TGATGGTCGAGGTTATCAACCAATGGTTTATGGGTTAGACAATTGTCAAATGAGTCAGACCAAAAGTATGGTAAAACTTG
TTTTTTATTTTAAGGACGGGTTAAAAAGGACATTTTACTATGATTTTAAAGAAGAAACTTAA

Domains

Predicted by InterProScan.

• Putative Competence protein ComGF (ComGF) (49-142)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Streptococcus mutans UA159	47.183	92.81	0.438
comGF	Streptococcus mutans UA140	47.183	92.81	0.438
comGF/cglF	Streptococcus pneumoniae Rx1	42.446	90.85	0.386
comGF/cglF	Streptococcus pneumoniae D39	42.446	90.85	0.386
comGF/cglF	Streptococcus pneumoniae R6	42.446	90.85	0.386
comGF/cglF	Streptococcus pneumoniae TIGR4	42.446	90.85	0.386
comGF/cglF	Streptococcus mitis SK321	43.284	87.582	0.379
comGF/cglF	Streptococcus mitis NCTC 12261	42.537	87.582	0.373
comGF	Lactococcus lactis subsp. cremoris KW2	40	91.503	0.366