Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   AMM49_RS01175 Genome accession   NZ_CP012480
Coordinates   197640..198068 (+) Length   142 a.a.
NCBI ID   WP_223842274.1    Uniprot ID   -
Organism   Streptococcus agalactiae strain NGBS128     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 192640..203068
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AMM49_RS01160 (AMM49_01155) - 195170..195535 (+) 366 WP_000285373.1 DUF1033 family protein -
  AMM49_RS10915 (AMM49_01160) comGA 195707..196678 (+) 972 Protein_164 competence type IV pilus ATPase ComGA -
  AMM49_RS01170 (AMM49_01165) comGB 196572..197615 (+) 1044 WP_223297804.1 competence type IV pilus assembly protein ComGB Machinery gene
  AMM49_RS01175 (AMM49_01170) comGF 197640..198068 (+) 429 WP_223842274.1 competence type IV pilus minor pilin ComGF Machinery gene
  AMM49_RS01180 (AMM49_01175) comGG 198046..198269 (+) 224 Protein_167 competence type IV pilus minor pilin ComGG -
  AMM49_RS01185 (AMM49_01180) comGH 198384..199358 (+) 975 WP_001008572.1 class I SAM-dependent methyltransferase Machinery gene
  AMM49_RS01190 (AMM49_01185) - 199390..200583 (+) 1194 WP_000047534.1 acetate kinase -
  AMM49_RS01195 (AMM49_01190) - 200734..200940 (+) 207 WP_000798241.1 helix-turn-helix transcriptional regulator -
  AMM49_RS11200 - 200999..201136 (+) 138 WP_001869149.1 hypothetical protein -
  AMM49_RS01200 (AMM49_01195) - 201177..201632 (+) 456 WP_000905672.1 hypothetical protein -
  AMM49_RS01205 (AMM49_01200) - 201701..202366 (+) 666 WP_000008107.1 CPBP family intramembrane glutamic endopeptidase -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 16590.29 Da        Isoelectric Point: 9.7864

>NTDB_id=133722 AMM49_RS01175 WP_223842274.1 197640..198068(+) (comGF) [Streptococcus agalactiae strain NGBS128]
MRRLKHLHILECLVALVTITGALLVYQGLTKLLAQQIVVMSSSSQSEWVLLTQQLNAEFEGAHLEYLRQNKLYLRKQDKI
VTFGKSNKDDFRKTGYNGRGYQPMVYGLDNCQMSQTKSMVKLVFYFKDGLKRTFYYDFKEET

Nucleotide


Download         Length: 429 bp        

>NTDB_id=133722 AMM49_RS01175 WP_223842274.1 197640..198068(+) (comGF) [Streptococcus agalactiae strain NGBS128]
ATAAGAAGGTTAAAGCATTTACACATTTTAGAATGTTTGGTAGCATTGGTTACAATCACAGGAGCTTTGCTAGTTTATCA
AGGACTAACAAAATTGTTGGCTCAACAGATAGTAGTGATGTCTTCTTCCAGTCAGTCTGAATGGGTGTTATTAACTCAGC
AACTAAATGCAGAATTCGAAGGCGCTCACTTGGAATATTTAAGACAGAACAAACTTTATTTACGTAAGCAAGATAAGATT
GTAACCTTTGGCAAATCTAATAAAGATGATTTCCGTAAGACAGGTTATAATGGTCGAGGTTATCAACCAATGGTTTATGG
GTTAGACAATTGTCAAATGAGTCAGACCAAAAGTATGGTAAAACTTGTTTTTTATTTTAAGGACGGGTTAAAAAGGACAT
TTTACTATGATTTTAAAGAAGAAACTTAA

Domains

Predicted by InterProScan.

Putative Competence protein ComGF (ComGF)

(38-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Streptococcus mutans UA159

44.355

87.324

0.387
  comGF Streptococcus mutans UA140

44.355

87.324

0.387
  comGF/cglF Streptococcus pneumoniae D39

39.568

97.887

0.387
  comGF/cglF Streptococcus pneumoniae TIGR4

39.568

97.887

0.387
  comGF/cglF Streptococcus pneumoniae R6

39.568

97.887

0.387
  comGF/cglF Streptococcus pneumoniae Rx1

39.568

97.887

0.387
  comGF/cglF Streptococcus mitis NCTC 12261

40.299

94.366

0.38
  comGF/cglF Streptococcus mitis SK321

40.299

94.366

0.38