Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   QI18_RS05630 Genome accession   NZ_CP010050
Coordinates   1102862..1104184 (-) Length   440 a.a.
NCBI ID   WP_039115354.1    Uniprot ID   -
Organism   Lactococcus lactis subsp. lactis strain S0 isolate fresh raw milk     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1097862..1109184
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QI18_RS05600 (QI18_05600) - 1099005..1099973 (+) 969 WP_003130649.1 PhoH family protein -
  QI18_RS05605 (QI18_05605) - 1100031..1100510 (+) 480 WP_039115350.1 NUDIX hydrolase -
  QI18_RS05610 (QI18_05610) ybeY 1100662..1101150 (+) 489 WP_021722516.1 rRNA maturation RNase YbeY -
  QI18_RS05615 (QI18_05615) - 1101134..1101589 (+) 456 WP_003130653.1 diacylglycerol kinase family protein -
  QI18_RS05620 (QI18_05620) - 1101603..1102181 (+) 579 WP_021722515.1 nucleotidyltransferase family protein -
  QI18_RS13105 comFC 1102215..1102619 (-) 405 WP_230593148.1 ComF family protein Machinery gene
  QI18_RS05630 (QI18_05630) comFA 1102862..1104184 (-) 1323 WP_039115354.1 DEAD/DEAH box helicase Machinery gene
  QI18_RS05635 (QI18_05635) - 1104241..1104870 (+) 630 WP_003130660.1 YigZ family protein -
  QI18_RS05640 (QI18_05640) - 1105044..1105502 (+) 459 WP_268988340.1 VanZ family protein -
  QI18_RS05645 (QI18_05645) - 1105550..1106569 (+) 1020 WP_012897709.1 DUF475 domain-containing protein -
  QI18_RS05650 (QI18_05650) - 1106684..1107430 (+) 747 WP_023189339.1 bifunctional metallophosphatase/5'-nucleotidase -
  QI18_RS05655 (QI18_05655) glyQ 1107716..1108669 (+) 954 WP_003130667.1 glycine--tRNA ligase subunit alpha -

Sequence

Protein


Download         Length: 440 a.a.        Molecular weight: 50088.55 Da        Isoelectric Point: 10.1065

>NTDB_id=133711 QI18_RS05630 WP_039115354.1 1102862..1104184(-) (comFA) [Lactococcus lactis subsp. lactis strain S0 isolate fresh raw milk]
MSTNQEKLFGRLLLKNDILQLIKSTDKISVSKIFSNFLLEAKVNPILGMTSISSNKIKCNRCGTVHIKNSVKLPIGVFYC
PSCIQLGRVRSDEFLYFLPQKNFPKKSYINWSGKLTENQKSISNALCQEINSHQQIIVQAVTGAGKTEMIYQVIEQILES
GGVVGLASPRIDVCLELHQRLSRDFSCKIPLLYHDGDSYFRAPLIIMTSHQLLRLKEAFDLLIIDEVDAFSFRDNEMLYF
AAEKARKIEGNLIYLTATSTDKLEKDIKKQKLYPLFLPRRFHNFPLVVPKFFWKNKFDKKLIEQRNSGFPLLIFAAEIES
GQEFAKQLQLKFPKEKIASVASTTKDRLEIVKAFRNKEITILIATSILERGVTFPNVDVFVINSEHPNFTKSALIQMAGR
VGRSPERPTGLVSFFHYGKSKAMCQAVREIKKMNQLGGFS

Nucleotide


Download         Length: 1323 bp        

>NTDB_id=133711 QI18_RS05630 WP_039115354.1 1102862..1104184(-) (comFA) [Lactococcus lactis subsp. lactis strain S0 isolate fresh raw milk]
ATGAGCACTAATCAAGAAAAGTTATTTGGCCGTTTATTATTAAAAAATGATATTTTACAACTTATAAAAAGTACTGACAA
GATTTCTGTCAGTAAAATTTTTAGTAATTTTTTGTTAGAAGCGAAGGTGAATCCAATTTTGGGAATGACTTCAATTTCTT
CCAATAAAATAAAATGCAACCGTTGTGGGACTGTTCATATAAAAAATTCTGTCAAACTTCCAATTGGTGTATTTTACTGT
CCAAGTTGTATTCAATTAGGTCGAGTCCGCTCCGATGAATTCTTGTACTTTCTGCCACAAAAGAATTTCCCAAAGAAATC
ATATATAAACTGGTCGGGAAAACTGACAGAGAATCAAAAATCAATTTCAAATGCCCTCTGTCAAGAAATTAATTCTCATC
AGCAAATAATTGTCCAAGCTGTGACTGGAGCTGGAAAAACTGAAATGATTTATCAAGTCATTGAACAAATTTTAGAAAGC
GGTGGAGTTGTTGGTCTAGCTAGTCCAAGAATTGATGTTTGTCTTGAACTTCATCAGCGATTATCACGTGATTTTTCCTG
TAAGATTCCACTCTTATATCATGATGGCGACAGCTATTTCCGAGCTCCATTAATAATAATGACCAGTCATCAGCTTTTAC
GTTTGAAGGAAGCTTTTGATTTGCTGATTATTGATGAAGTTGATGCCTTTTCCTTTAGAGATAATGAAATGCTTTATTTT
GCGGCAGAAAAAGCAAGAAAAATAGAAGGAAATTTAATATATTTGACCGCAACTTCTACTGACAAACTTGAAAAAGATAT
AAAAAAGCAAAAACTCTATCCTTTGTTTCTCCCACGTCGTTTTCACAATTTTCCTTTAGTCGTGCCTAAATTTTTTTGGA
AAAATAAATTTGATAAGAAATTAATTGAGCAAAGAAATAGTGGCTTTCCTCTTCTTATTTTTGCTGCTGAAATTGAATCT
GGACAAGAATTTGCAAAACAACTACAATTGAAATTTCCTAAAGAAAAAATTGCTTCCGTTGCTTCAACAACAAAAGATAG
ATTGGAAATTGTTAAAGCTTTTAGAAATAAAGAAATTACTATTTTAATAGCGACTTCAATTCTTGAACGGGGAGTCACTT
TTCCAAATGTTGATGTTTTTGTCATCAACAGTGAGCACCCAAACTTCACTAAATCCGCACTGATACAAATGGCTGGACGT
GTGGGTCGTAGTCCTGAACGTCCAACAGGCTTAGTTAGTTTTTTTCATTACGGAAAATCCAAAGCAATGTGTCAGGCAGT
TAGAGAAATCAAAAAAATGAACCAACTGGGAGGTTTTTCATGA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(300-403)

Type III restriction enzyme, res subunit (ResIII)

(114-258)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Lactococcus lactis subsp. cremoris KW2

74

90.909

0.673
  comFA/cflA Streptococcus mitis SK321

51.508

90.455

0.466
  comFA/cflA Streptococcus pneumoniae Rx1

51.508

90.455

0.466
  comFA/cflA Streptococcus pneumoniae D39

51.508

90.455

0.466
  comFA/cflA Streptococcus pneumoniae R6

51.508

90.455

0.466
  comFA/cflA Streptococcus pneumoniae TIGR4

51.508

90.455

0.466
  comFA/cflA Streptococcus mitis NCTC 12261

50.754

90.455

0.459
  comFA Bacillus subtilis subsp. subtilis str. 168

37.029

100

0.38

Multiple sequence alignment