Detailed information NTG0133203

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: LT85_RS22130
• Genome accession: NZ_CP009962
• Coordinates: 5017309..5018175 (+)
• Length: 288 a.a.
• NCBI ID: WP_038493260.1
• Uniprot ID: A0A0A1FIU3
• Organism: Collimonas arenae strain Cal35
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5012309..5023175

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LT85_RS22115 (LT85_4469)	-	5012928..5014217 (+)	1290	WP_038493254.1	HlyC/CorC family transporter	-
LT85_RS22120 (LT85_4470)	pilB	5014340..5016061 (+)	1722	WP_038493256.1	type IV-A pilus assembly ATPase PilB	Machinery gene
LT85_RS22125 (LT85_4471)	pilC	5016077..5017309 (+)	1233	WP_038493258.1	type II secretion system F family protein	Machinery gene
LT85_RS22130 (LT85_4472)	pilD	5017309..5018175 (+)	867	WP_038493260.1	A24 family peptidase	Machinery gene
LT85_RS22135 (LT85_4473)	coaE	5018182..5018832 (+)	651	WP_038493262.1	dephospho-CoA kinase	-
LT85_RS22140 (LT85_4474)	zapD	5018963..5019718 (+)	756	WP_038493264.1	cell division protein ZapD	-
LT85_RS22145 (LT85_4475)	-	5019741..5019929 (+)	189	WP_038493266.1	DNA gyrase inhibitor YacG	-
LT85_RS22150 (LT85_4476)	-	5020004..5020417 (-)	414	WP_038493267.1	NUDIX domain-containing protein	-
LT85_RS22155 (LT85_4477)	-	5020438..5021316 (-)	879	WP_038493270.1	ATP-binding protein	-
LT85_RS22160 (LT85_4478)	argJ	5021493..5022731 (-)	1239	WP_038493273.1	bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ	-

Sequence

Protein

• Length: 288 a.a.
• Molecular weight: 31211.06 Da
• Isoelectric Point: 7.7587

>NTDB_id=133203 LT85_RS22130 WP_038493260.1 5017309..5018175(+) (pilD) [Collimonas arenae strain Cal35]
MQDGIFFLAAGSLLPTVLAAVFGLLIGSFLNVVIHRLPIMMQRESDNYVAHESGKPLPHTDRYNLIVPRSACPQCKHQIG
ALENVPVLSYLALRGKCASCKTPISARYPIVETFTGALSALLIWHFGSGWVGLATLVFAYLLIAMTFIDADTQLLPDDLT
LPLLWLGLLLNLSGLFVPLNEAVIGAAAGYLSLWTIYWAFKLLTGKEGMGYGDFKLLAALGAWLGWKMLPIIILFSSLVG
AIVGIALIIFARRGRDNPIPFGPYLAAAGVLALLYGHTILETYFGFAT

Nucleotide

• Length: 867 bp

>NTDB_id=133203 LT85_RS22130 WP_038493260.1 5017309..5018175(+) (pilD) [Collimonas arenae strain Cal35]
ATGCAAGATGGTATTTTTTTCCTGGCGGCGGGAAGCCTGCTTCCCACCGTTCTGGCAGCTGTTTTTGGCTTGCTTATCGG
CAGTTTTCTGAATGTAGTGATTCATCGCTTGCCGATCATGATGCAGCGCGAATCGGATAATTATGTGGCCCATGAAAGCG
GCAAGCCGCTGCCGCATACCGATCGCTACAACCTGATCGTGCCGCGTTCCGCCTGTCCGCAATGCAAACACCAGATCGGC
GCATTGGAAAACGTGCCGGTGCTGAGCTACTTGGCATTGCGTGGTAAATGCGCATCCTGCAAAACGCCAATTTCCGCACG
CTATCCGATTGTGGAAACATTTACCGGTGCCTTGTCTGCGCTGCTGATCTGGCATTTCGGCAGCGGCTGGGTTGGTTTGG
CGACTCTGGTTTTTGCCTATCTGCTGATTGCAATGACGTTCATCGATGCCGATACGCAGTTGCTGCCCGATGACCTGACC
CTACCCTTGTTATGGCTAGGTTTGCTACTCAATCTGTCAGGCCTGTTCGTACCGCTGAATGAGGCCGTCATTGGCGCTGC
TGCCGGCTATCTGAGCTTGTGGACGATTTACTGGGCATTCAAACTGCTGACTGGCAAGGAAGGCATGGGCTACGGTGATT
TCAAGCTGCTAGCGGCGCTGGGCGCATGGCTGGGCTGGAAAATGCTCCCCATTATTATCTTGTTTTCATCGCTGGTAGGC
GCCATTGTCGGCATCGCGTTGATCATTTTTGCTCGCCGCGGACGCGACAATCCGATCCCGTTTGGCCCCTATCTGGCGGC
TGCCGGCGTACTGGCTCTACTTTATGGCCACACAATTCTGGAAACTTATTTCGGTTTTGCTACCTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (136-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-126)

3D structure

• AlphaFold DB: A0A0A1FIU3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	56.458	94.097	0.531
pilD	Vibrio campbellii strain DS40M4	54.044	94.444	0.51
pilD	Acinetobacter baumannii D1279779	47.038	99.653	0.469
pilD	Neisseria gonorrhoeae MS11	52.756	88.194	0.465
pilD	Acinetobacter nosocomialis M2	46.341	99.653	0.462