Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   AKN41_RS09885 Genome accession   NZ_CP012376
Coordinates   2029708..2030718 (-) Length   336 a.a.
NCBI ID   WP_000568519.1    Uniprot ID   Q32HA1
Organism   Escherichia coli strain GE3     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2024708..2035718
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AKN41_RS09865 (AKN41_1977) mepM 2025675..2026997 (-) 1323 WP_001184045.1 murein DD-endopeptidase MepM -
  AKN41_RS09870 (AKN41_1978) znuA 2027013..2027945 (-) 933 WP_001342995.1 zinc ABC transporter substrate-binding protein ZnuA -
  AKN41_RS09875 (AKN41_1979) znuC 2028024..2028779 (+) 756 WP_000202996.1 zinc ABC transporter ATP-binding protein ZnuC -
  AKN41_RS09880 (AKN41_1980) znuB 2028776..2029561 (+) 786 WP_000571465.1 zinc ABC transporter permease subunit ZnuB -
  AKN41_RS09885 (AKN41_1981) ruvB 2029708..2030718 (-) 1011 WP_000568519.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  AKN41_RS09890 (AKN41_1982) ruvA 2030727..2031338 (-) 612 WP_000580323.1 Holliday junction branch migration protein RuvA -
  AKN41_RS09895 yobI 2031477..2031542 (-) 66 WP_001386853.1 stress response small protein YobI -
  AKN41_RS09900 (AKN41_1983) yebB 2031613..2032215 (+) 603 WP_001024930.1 YebB family permuted papain-like enzyme -
  AKN41_RS09905 (AKN41_1984) ruvC 2032217..2032738 (-) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  AKN41_RS09910 (AKN41_1985) yebC 2032773..2033513 (-) 741 WP_000907234.1 YebC/PmpR family DNA-binding transcriptional regulator -
  AKN41_RS09915 (AKN41_1986) nudB 2033542..2033994 (-) 453 WP_077221229.1 dihydroneopterin triphosphate diphosphatase -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37173.77 Da        Isoelectric Point: 4.7818

>NTDB_id=133103 AKN41_RS09885 WP_000568519.1 2029708..2030718(-) (ruvB) [Escherichia coli strain GE3]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TTRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=133103 AKN41_RS09885 WP_000568519.1 2029708..2030718(-) (ruvB) [Escherichia coli strain GE3]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTGGCAGATCGCGCCATTCGCCCTAAATT
ATTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATCTGTTGATTTTCGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAACATCGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATCTGGCAGCGATGCTCACTAACCTTGAACCGCA
TGACGTGTTGTTTATTGATGAGATCCACCGTCTTTCGCCAGTGGTGGAAGAGGTGTTGTATCCGGCAATGGAAGACTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGATCGTTTTGGTATTGTGCAGCGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGAGCTCGCGGTACGCCGCGCATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGTTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
GTTATTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACCTTGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTGCTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG
ACGACGCGGGCGTGGAATCACTTTGGCATAACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q32HA1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

53.251

96.131

0.512
  ruvB Helicobacter pylori 26695

52.038

94.94

0.494

Multiple sequence alignment