Detailed information NTG0132931

Bioinformatically predicted

Overview

• Name: comYF
• Type: Machinery gene
• Locus tag: SSAL8618_RS09505
• Genome accession: NZ_CP009913
• Coordinates: 2049642..2050079 (-)
• Length: 145 a.a.
• NCBI ID: WP_002887014.1
• Uniprot ID: A0A1V0GF29
• Organism: Streptococcus salivarius strain NCTC 8618
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2044642..2055079

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SSAL8618_RS09470 (SSAL8618_09470)	-	2044684..2045346 (-)	663	WP_002887009.1	CPBP family intramembrane glutamic endopeptidase	-
SSAL8618_RS09475 (SSAL8618_09475)	-	2045459..2045905 (-)	447	WP_037605837.1	hypothetical protein	-
SSAL8618_RS09480 (SSAL8618_09480)	-	2045979..2046635 (-)	657	WP_002887011.1	CPBP family intramembrane glutamic endopeptidase	-
SSAL8618_RS09485 (SSAL8618_09485)	-	2046647..2046844 (-)	198	WP_002885716.1	helix-turn-helix transcriptional regulator	-
SSAL8618_RS09490 (SSAL8618_09490)	-	2047096..2048289 (-)	1194	WP_002885831.1	acetate kinase	-
SSAL8618_RS09495 (SSAL8618_09495)	comYH	2048346..2049302 (-)	957	WP_038676858.1	class I SAM-dependent methyltransferase	Machinery gene
SSAL8618_RS09500 (SSAL8618_09500)	comGG	2049347..2049664 (-)	318	WP_002887013.1	competence type IV pilus minor pilin ComGG	-
SSAL8618_RS09505 (SSAL8618_09505)	comYF	2049642..2050079 (-)	438	WP_002887014.1	competence type IV pilus minor pilin ComGF	Machinery gene
SSAL8618_RS09510 (SSAL8618_09510)	comGE	2050066..2050296 (-)	231	WP_002887015.1	competence type IV pilus minor pilin ComGE	-
SSAL8618_RS09515 (SSAL8618_09515)	comYD	2050328..2050756 (-)	429	WP_254593949.1	competence type IV pilus minor pilin ComGD	Machinery gene
SSAL8618_RS09520 (SSAL8618_09520)	comYC	2050716..2051030 (-)	315	WP_170314557.1	competence type IV pilus major pilin ComGC	Machinery gene
SSAL8618_RS09525 (SSAL8618_09525)	comYB	2051039..2052139 (-)	1101	WP_126405090.1	competence type IV pilus assembly protein ComGB	Machinery gene
SSAL8618_RS09530 (SSAL8618_09530)	comGA/cglA/cilD	2052021..2052962 (-)	942	WP_038676863.1	competence type IV pilus ATPase ComGA	Machinery gene
SSAL8618_RS09535 (SSAL8618_09535)	-	2053042..2053404 (-)	363	WP_002887019.1	DUF1033 family protein	-

Sequence

Protein

• Length: 145 a.a.
• Molecular weight: 16155.57 Da
• Isoelectric Point: 8.4658

>NTDB_id=132931 SSAL8618_RS09505 WP_002887014.1 2049642..2050079(-) (comYF) [Streptococcus salivarius strain NCTC 8618]
MSLNSKVRAFTLLECLVALLVIAGSVQVYQGLTTVLVSNVKQVKQQESQDWLLFVQQMEAELEGCQLVKVEGNKLYVKQD
NQDLAFGLSSATDFRKTNADGRGYQPMLFNVKASTISQNNQIVTIQVTLKNGLQRSFVYAFEKTG

Nucleotide

• Length: 438 bp

>NTDB_id=132931 SSAL8618_RS09505 WP_002887014.1 2049642..2050079(-) (comYF) [Streptococcus salivarius strain NCTC 8618]
ATGTCTCTCAATAGCAAGGTTCGTGCTTTTACCCTCTTGGAATGTCTAGTGGCCTTGTTGGTGATTGCTGGTTCAGTTCA
AGTTTATCAAGGTCTGACCACAGTATTGGTGAGCAATGTTAAGCAGGTCAAGCAACAAGAAAGCCAAGACTGGCTTCTAT
TCGTTCAACAAATGGAGGCAGAATTAGAAGGTTGTCAGTTGGTAAAGGTAGAGGGGAACAAACTCTATGTCAAGCAGGAT
AATCAAGACTTAGCTTTTGGACTATCAAGTGCTACTGATTTTCGAAAAACAAATGCTGACGGTCGAGGCTACCAACCCAT
GCTTTTTAATGTAAAAGCTAGTACAATTAGCCAAAATAACCAGATAGTCACCATCCAAGTGACTCTTAAAAATGGGCTAC
AAAGGAGTTTTGTTTATGCTTTTGAAAAAACAGGTTAG

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (39-134)

3D structure

• AlphaFold DB: A0A1V0GF29

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comYF	Streptococcus mutans UA140	58.156	97.241	0.566
comYF	Streptococcus mutans UA159	57.447	97.241	0.559
comGF/cglF	Streptococcus pneumoniae Rx1	50	97.931	0.49
comGF/cglF	Streptococcus pneumoniae D39	50	97.931	0.49
comGF/cglF	Streptococcus pneumoniae R6	50	97.931	0.49
comGF/cglF	Streptococcus pneumoniae TIGR4	50	97.931	0.49
comGF/cglF	Streptococcus mitis SK321	47.222	99.31	0.469
comGF/cglF	Streptococcus mitis NCTC 12261	47.222	99.31	0.469
comGF	Lactococcus lactis subsp. cremoris KW2	45.324	95.862	0.434