Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   OM33_RS13755 Genome accession   NZ_CP009888
Coordinates   2978050..2979180 (-) Length   376 a.a.
NCBI ID   WP_038642519.1    Uniprot ID   A0A0A7EHG7
Organism   Pseudoalteromonas piratica strain OCN003     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2973050..2984180
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OM33_RS13725 (OM33_13725) - 2973298..2974197 (-) 900 WP_038642507.1 ABC transporter ATP-binding protein -
  OM33_RS13730 (OM33_13730) rsmE 2974271..2975014 (+) 744 WP_038642509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OM33_RS13735 (OM33_13735) gshB 2975069..2976028 (+) 960 WP_038642511.1 glutathione synthase -
  OM33_RS13740 (OM33_13740) - 2976053..2976622 (+) 570 WP_038642513.1 YqgE/AlgH family protein -
  OM33_RS13745 (OM33_13745) ruvX 2976623..2977063 (+) 441 WP_038642515.1 Holliday junction resolvase RuvX -
  OM33_RS13750 (OM33_13750) - 2977139..2978035 (-) 897 WP_038642517.1 hypothetical protein -
  OM33_RS13755 (OM33_13755) pilU 2978050..2979180 (-) 1131 WP_038642519.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OM33_RS13760 (OM33_13760) pilT 2979196..2980248 (-) 1053 WP_038642520.1 type IV pilus twitching motility protein PilT Machinery gene
  OM33_RS13765 (OM33_13765) - 2980286..2980972 (+) 687 WP_038642521.1 YggS family pyridoxal phosphate-dependent enzyme -
  OM33_RS13770 (OM33_13770) proC 2980996..2981817 (+) 822 WP_038642523.1 pyrroline-5-carboxylate reductase -
  OM33_RS13775 (OM33_13775) - 2981817..2982353 (+) 537 WP_038642525.1 YggT family protein -
  OM33_RS13780 (OM33_13780) - 2982445..2982885 (+) 441 WP_038642527.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41894.71 Da        Isoelectric Point: 6.1503

>NTDB_id=132722 OM33_RS13755 WP_038642519.1 2978050..2979180(-) (pilU) [Pseudoalteromonas piratica strain OCN003]
MYNLDHFLRQMVENEASDLFVSTHLPVSAKINGELRPVGENDLTEEEALAMVESAMTEKQIKEFHDTKECNFAIARDSGR
FRVSAFWQRDKAGMVIRRIVTTIPEVQALGLPTVLTDVIMSKRGLVLFVGGTGTGKSTSLAALLGYRNRNQRGHILTIED
PIEFVHEHQKSIVTQREVGLDTDSFESALKSSLRQAPDVILIGEIRSQETMEYALSFAETGHLCVATLHANNANQAIDRI
MHLVPKEKHDKLKYDLALNLRAIVAQQLVPTSDGHGRAAAIEILLNSPMVAELIKRGEVGAIKETMSKSREMGMQTFDQA
LFDLYQQHRINYADALHHADSPNDLRLMIKLRNNEQQGAGFLQGVTVDGLDGDKNQ

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=132722 OM33_RS13755 WP_038642519.1 2978050..2979180(-) (pilU) [Pseudoalteromonas piratica strain OCN003]
ATGTACAATCTTGATCATTTTTTAAGACAAATGGTAGAGAATGAAGCATCGGATTTATTCGTTTCTACACATCTACCAGT
CAGTGCCAAAATTAATGGTGAATTACGCCCTGTAGGCGAAAACGACTTAACCGAAGAAGAAGCCCTTGCGATGGTTGAGT
CGGCAATGACAGAAAAGCAAATTAAAGAGTTCCACGACACGAAAGAGTGTAACTTTGCAATTGCACGCGATTCAGGTCGT
TTCCGTGTCTCAGCCTTTTGGCAGCGAGATAAAGCTGGCATGGTTATTCGTCGAATTGTCACGACTATCCCTGAAGTACA
AGCACTTGGCTTACCAACAGTATTAACTGATGTGATTATGTCAAAACGTGGCTTGGTGCTATTTGTAGGTGGTACGGGTA
CTGGTAAGTCAACTTCATTAGCAGCCCTTTTAGGTTACCGTAACCGTAATCAGCGTGGTCATATTTTAACCATTGAAGAT
CCCATTGAATTTGTGCACGAACACCAGAAAAGCATTGTGACCCAGCGAGAAGTAGGTCTTGATACAGATAGCTTTGAATC
AGCGCTGAAAAGCTCACTTCGTCAGGCTCCAGATGTTATTCTGATTGGTGAGATCCGTTCGCAAGAAACCATGGAATACG
CGCTCTCTTTTGCTGAAACTGGTCACTTATGTGTGGCAACATTGCACGCCAATAACGCAAACCAAGCAATCGACCGTATT
ATGCACTTAGTGCCAAAAGAAAAGCATGACAAGTTAAAATACGATTTAGCCCTTAACTTACGTGCCATTGTAGCGCAACA
ATTAGTACCAACCAGTGATGGCCATGGCCGTGCAGCTGCGATTGAAATATTACTCAACTCGCCCATGGTTGCTGAACTAA
TTAAACGTGGCGAAGTGGGGGCAATTAAAGAAACCATGTCGAAATCTCGCGAGATGGGTATGCAAACATTCGACCAAGCG
CTTTTTGATCTCTATCAGCAACACCGTATTAATTATGCTGATGCACTGCATCATGCTGATTCGCCAAATGACTTACGATT
GATGATTAAACTTCGTAATAATGAGCAACAGGGTGCCGGTTTCTTGCAAGGGGTGACTGTTGATGGTCTTGATGGAGATA
AAAACCAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0A7EHG7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.358

99.468

0.59
  pilU Vibrio cholerae strain A1552

57.534

97.074

0.559
  pilU Acinetobacter baylyi ADP1

55.053

100

0.551
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.061

95.479

0.402

Multiple sequence alignment