Detailed information NTG0132686

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: XFHB_RS08245
• Genome accession: NZ_CP009885
• Coordinates: 1626070..1627200 (+)
• Length: 376 a.a.
• NCBI ID: WP_088578148.1
• Uniprot ID: -
• Organism: Xylella fastidiosa strain Hib4
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1621070..1632200

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
XFHB_RS08220 (XFHB_07765)	-	1621292..1622116 (+)	825	WP_023907665.1	glycosyltransferase family 2 protein	-
XFHB_RS08230 (XFHB_07770)	-	1622780..1623607 (-)	828	WP_194955730.1	glycosyltransferase family 2 protein	-
XFHB_RS08240 (XFHB_07775)	pilT	1624870..1625904 (+)	1035	WP_004084948.1	type IV pilus twitching motility protein PilT	Machinery gene
XFHB_RS08245 (XFHB_07780)	pilU	1626070..1627200 (+)	1131	WP_088578148.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
XFHB_RS08250 (XFHB_07785)	-	1627717..1628705 (-)	989	Protein_1543	YafY family protein	-
XFHB_RS08260 (XFHB_07790)	-	1629549..1630358 (+)	810	WP_031336664.1	DUF481 domain-containing protein	-
XFHB_RS08265 (XFHB_07795)	hemC	1630412..1631329 (-)	918	WP_010894123.1	hydroxymethylbilane synthase	-
XFHB_RS08270 (XFHB_07800)	-	1631346..1632104 (-)	759	WP_010894122.1	LytTR family DNA-binding domain-containing protein	-

Sequence

Protein

• Length: 376 a.a.
• Molecular weight: 42417.60 Da
• Isoelectric Point: 6.3881

>NTDB_id=132686 XFHB_RS08245 WP_088578148.1 1626070..1627200(+) (pilU) [Xylella fastidiosa strain Hib4]
MTTIDFTSLLKLMAHQKASDLFITCGMPPSMKIHGNITPITQVPLTSEQSRDIVFNIMSEAQREEFEKVHECNFAIDITN
IGRFRVNAFYQRNQIGMVLRRIEMSIPTFEKLKLPQVAKELVMEKRGIIIIVGATGSGKSTSMASMINHRNHNSTGHIVT
IEDPIEFIHKHERCIITQREIGTDTDSWENALKNTLRQAPDVIVIGEVRTQETMDHAISFSETGHLVMCTLHANNANQAI
DRIINFFPEERRSQLLTDLSLNLKGIIAQQLIPSPDGKERCLAAEVLRDSPLVQDYIRVGEIHKLKEVMQNPNDLGMTTF
DQSLVELYHSGAINYKEALRHADSQNEVRLRIKLSDGKDAKTLSQGFDGVEIAEMS

Nucleotide

• Length: 1131 bp

>NTDB_id=132686 XFHB_RS08245 WP_088578148.1 1626070..1627200(+) (pilU) [Xylella fastidiosa strain Hib4]
ATGACTACCATCGATTTCACATCCTTACTTAAGCTGATGGCGCACCAAAAAGCGTCGGATTTATTTATTACCTGTGGAAT
GCCGCCTTCAATGAAGATTCACGGCAATATCACTCCAATCACACAAGTACCGCTGACTTCAGAGCAAAGCCGTGACATAG
TCTTCAATATCATGAGTGAAGCGCAGCGCGAGGAATTCGAGAAAGTCCATGAATGCAACTTCGCAATTGACATCACCAAC
ATTGGTCGCTTCCGCGTCAACGCTTTCTATCAGCGTAACCAAATTGGTATGGTGTTACGCAGAATCGAGATGTCTATTCC
CACCTTCGAAAAACTGAAGCTCCCGCAGGTCGCTAAGGAGCTCGTCATGGAGAAGCGCGGCATCATCATCATCGTGGGTG
CCACGGGTAGTGGTAAATCGACCTCAATGGCATCAATGATCAACCACCGTAATCACAACTCAACAGGACATATCGTCACT
ATCGAAGATCCCATTGAGTTTATTCACAAGCACGAGAGATGCATCATCACCCAACGCGAAATAGGTACCGACACCGACAG
TTGGGAAAATGCCTTGAAAAATACCCTACGCCAAGCCCCAGATGTGATTGTGATTGGTGAGGTGCGTACTCAAGAGACAA
TGGATCATGCCATCTCGTTCTCAGAAACCGGCCATCTGGTCATGTGTACCCTACATGCCAACAACGCCAATCAAGCAATA
GACCGCATTATTAACTTCTTCCCGGAAGAGCGGCGCAGCCAATTACTGACTGATCTGTCGCTGAATCTAAAAGGCATCAT
CGCTCAACAATTGATCCCATCTCCGGATGGTAAAGAGCGCTGTTTAGCAGCGGAAGTTTTGCGCGACAGCCCATTAGTAC
AGGACTACATACGCGTAGGCGAGATCCATAAGCTCAAAGAAGTCATGCAAAACCCCAACGATCTTGGAATGACTACATTC
GATCAAAGCTTGGTCGAACTCTATCATAGCGGAGCCATTAACTATAAAGAGGCACTACGCCATGCTGATTCGCAGAACGA
GGTACGCCTGCGTATTAAACTCTCCGATGGAAAAGACGCCAAAACTCTGAGTCAAGGATTCGATGGAGTAGAAATTGCAG
AAATGTCATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-272)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.853	97.606	0.604
pilU	Acinetobacter baylyi ADP1	60.169	94.149	0.566
pilU	Vibrio cholerae strain A1552	51.404	94.681	0.487
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.484	96.809	0.402