Detailed information NTG0132684

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: XFHB_RS07700
• Genome accession: NZ_CP009885
• Coordinates: 1536692..1537822 (+)
• Length: 376 a.a.
• NCBI ID: WP_088578148.1
• Uniprot ID: -
• Organism: Xylella fastidiosa strain Hib4
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1531692..1542822

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
XFHB_RS07675 (XFHB_07230)	-	1531914..1532738 (+)	825	WP_023907665.1	glycosyltransferase family 2 protein	-
XFHB_RS07685 (XFHB_07235)	-	1533402..1534229 (-)	828	WP_194955730.1	glycosyltransferase family 2 protein	-
XFHB_RS07695 (XFHB_07240)	pilT	1535492..1536526 (+)	1035	WP_004084948.1	type IV pilus twitching motility protein PilT	Machinery gene
XFHB_RS07700 (XFHB_07245)	pilU	1536692..1537822 (+)	1131	WP_088578148.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
XFHB_RS07705 (XFHB_07250)	-	1538339..1539327 (-)	989	Protein_1438	YafY family protein	-
XFHB_RS07715 (XFHB_07255)	-	1540171..1540980 (+)	810	WP_031336664.1	DUF481 domain-containing protein	-
XFHB_RS07720 (XFHB_07260)	hemC	1541034..1541951 (-)	918	WP_010894123.1	hydroxymethylbilane synthase	-
XFHB_RS07725 (XFHB_07265)	-	1541968..1542726 (-)	759	WP_010894122.1	LytTR family DNA-binding domain-containing protein	-

Sequence

Protein

• Length: 376 a.a.
• Molecular weight: 42417.60 Da
• Isoelectric Point: 6.3881

>NTDB_id=132684 XFHB_RS07700 WP_088578148.1 1536692..1537822(+) (pilU) [Xylella fastidiosa strain Hib4]
MTTIDFTSLLKLMAHQKASDLFITCGMPPSMKIHGNITPITQVPLTSEQSRDIVFNIMSEAQREEFEKVHECNFAIDITN
IGRFRVNAFYQRNQIGMVLRRIEMSIPTFEKLKLPQVAKELVMEKRGIIIIVGATGSGKSTSMASMINHRNHNSTGHIVT
IEDPIEFIHKHERCIITQREIGTDTDSWENALKNTLRQAPDVIVIGEVRTQETMDHAISFSETGHLVMCTLHANNANQAI
DRIINFFPEERRSQLLTDLSLNLKGIIAQQLIPSPDGKERCLAAEVLRDSPLVQDYIRVGEIHKLKEVMQNPNDLGMTTF
DQSLVELYHSGAINYKEALRHADSQNEVRLRIKLSDGKDAKTLSQGFDGVEIAEMS

Nucleotide

• Length: 1131 bp

>NTDB_id=132684 XFHB_RS07700 WP_088578148.1 1536692..1537822(+) (pilU) [Xylella fastidiosa strain Hib4]
ATGACTACCATCGATTTCACATCCTTACTTAAGCTGATGGCGCACCAAAAAGCGTCGGATTTATTTATTACCTGTGGAAT
GCCGCCTTCAATGAAGATTCACGGCAATATCACTCCAATCACACAAGTACCGCTGACTTCAGAGCAAAGCCGTGACATAG
TCTTCAATATCATGAGTGAAGCGCAGCGCGAGGAATTCGAGAAAGTCCATGAATGCAACTTCGCAATTGACATCACCAAC
ATTGGTCGCTTCCGCGTCAACGCTTTCTATCAGCGTAACCAAATTGGTATGGTGTTACGCAGAATCGAGATGTCTATTCC
CACCTTCGAAAAACTGAAGCTCCCGCAGGTCGCTAAGGAGCTCGTCATGGAGAAGCGCGGCATCATCATCATCGTGGGTG
CCACGGGTAGTGGTAAATCGACCTCAATGGCATCAATGATCAACCACCGTAATCACAACTCAACAGGACATATCGTCACT
ATCGAAGATCCCATTGAGTTTATTCACAAGCACGAGAGATGCATCATCACCCAACGCGAAATAGGTACCGACACCGACAG
TTGGGAAAATGCCTTGAAAAATACCCTACGCCAAGCCCCAGATGTGATTGTGATTGGTGAGGTGCGTACTCAAGAGACAA
TGGATCATGCCATCTCGTTCTCAGAAACCGGCCATCTGGTCATGTGTACCCTACATGCCAACAACGCCAATCAAGCAATA
GACCGCATTATTAACTTCTTCCCGGAAGAGCGGCGCAGCCAATTACTGACTGATCTGTCGCTGAATCTAAAAGGCATCAT
CGCTCAACAATTGATCCCATCTCCGGATGGTAAAGAGCGCTGTTTAGCAGCGGAAGTTTTGCGCGACAGCCCATTAGTAC
AGGACTACATACGCGTAGGCGAGATCCATAAGCTCAAAGAAGTCATGCAAAACCCCAACGATCTTGGAATGACTACATTC
GATCAAAGCTTGGTCGAACTCTATCATAGCGGAGCCATTAACTATAAAGAGGCACTACGCCATGCTGATTCGCAGAACGA
GGTACGCCTGCGTATTAAACTCTCCGATGGAAAAGACGCCAAAACTCTGAGTCAAGGATTCGATGGAGTAGAAATTGCAG
AAATGTCATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-272)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.853	97.606	0.604
pilU	Acinetobacter baylyi ADP1	60.169	94.149	0.566
pilU	Vibrio cholerae strain A1552	51.404	94.681	0.487
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.484	96.809	0.402