Detailed information NTG0132457

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: XFC3_RS06625
• Genome accession: NZ_CP009829
• Coordinates: 1437228..1438358 (+)
• Length: 376 a.a.
• NCBI ID: WP_010894128.1
• Uniprot ID: Q9PCX2
• Organism: Xylella fastidiosa strain 3124
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1432228..1443358

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
XFC3_RS06605 (XFC3_06605)	-	1432434..1433258 (+)	825	WP_023906628.1	glycosyltransferase family 2 protein	-
XFC3_RS12695	-	1433422..1433781 (-)	360	WP_233242640.1	hypothetical protein	-
XFC3_RS06615 (XFC3_06615)	-	1433933..1434760 (-)	828	WP_031337845.1	glycosyltransferase family 2 protein	-
XFC3_RS06620 (XFC3_06630)	-	1436023..1437062 (+)	1040	Protein_1306	type IV pilus twitching motility protein PilT	-
XFC3_RS06625 (XFC3_06635)	pilU	1437228..1438358 (+)	1131	WP_010894128.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
XFC3_RS06630 (XFC3_06640)	-	1438875..1439888 (-)	1014	WP_229716473.1	YafY family protein	-
XFC3_RS06635 (XFC3_06645)	-	1440732..1441541 (+)	810	WP_031336664.1	DUF481 domain-containing protein	-
XFC3_RS06640 (XFC3_06650)	hemC	1441595..1442512 (-)	918	WP_010894123.1	hydroxymethylbilane synthase	-
XFC3_RS06645 (XFC3_06655)	-	1442529..1443287 (-)	759	WP_010894122.1	LytTR family DNA-binding domain-containing protein	-

Sequence

Protein

• Length: 376 a.a.
• Molecular weight: 42476.67 Da
• Isoelectric Point: 6.5027

>NTDB_id=132457 XFC3_RS06625 WP_010894128.1 1437228..1438358(+) (pilU) [Xylella fastidiosa strain 3124]
MTTIDFTSLLKLMAHQKASDLFITCGMPPSMKIHGNITPITQVPLTSEQSRDIVFNIMSEAQREEFEKVHECNFAIDITN
IGRFRVNAFYQRNQIGMVLRRIEMSIPTFEKLKLPQVAKELVMEKRGIIIIVGATGSGKSTSMASMINHRNHNSTGHIVT
IEDPIEFIHKHERCIITQREIGTDTDSWENALKNTLRQAPDVIVIGEVRTQETMDHAISFSETGHLVMCTLHANNANQAI
DRIINFFPEERRSQLLTDLSLNLKGIIAQQLIPSPDGKERCLAAEVLRDSRLVQDYIRVGEIHKLKEVMQNPNDLGMTTF
DQSLVELYHSGAINYKEALRHADSQNEVRLRIKLSDGKDAKTLSQGFDGVEIAEMS

Nucleotide

• Length: 1131 bp

>NTDB_id=132457 XFC3_RS06625 WP_010894128.1 1437228..1438358(+) (pilU) [Xylella fastidiosa strain 3124]
ATGACTACCATCGATTTCACATCCTTACTTAAGCTGATGGCGCACCAAAAAGCGTCGGATTTATTTATTACCTGTGGAAT
GCCGCCTTCAATGAAGATTCACGGCAATATCACTCCAATCACACAAGTACCGCTGACTTCAGAGCAAAGCCGTGACATAG
TCTTCAATATCATGAGTGAAGCGCAGCGCGAGGAATTCGAGAAAGTCCATGAATGCAACTTCGCAATTGACATCACCAAC
ATTGGTCGCTTCCGCGTCAACGCTTTCTATCAGCGTAACCAAATTGGTATGGTGTTACGCAGAATCGAGATGTCTATTCC
CACCTTCGAAAAACTGAAGCTCCCGCAGGTCGCTAAGGAGCTCGTCATGGAGAAGCGCGGCATCATCATCATCGTGGGTG
CCACGGGTAGTGGTAAATCGACCTCAATGGCATCAATGATCAACCACCGTAATCACAACTCAACAGGACATATCGTCACT
ATCGAAGATCCCATTGAGTTTATTCACAAGCACGAGAGATGCATCATCACTCAACGCGAAATAGGTACCGACACCGACAG
TTGGGAAAATGCCTTGAAAAATACCCTACGCCAAGCCCCAGATGTGATTGTGATTGGTGAGGTGCGTACTCAAGAGACAA
TGGATCATGCCATCTCGTTCTCAGAAACCGGCCATCTGGTCATGTGTACCCTACATGCCAACAACGCCAATCAAGCAATA
GACCGCATTATTAACTTCTTCCCGGAAGAGCGGCGCAGCCAATTACTGACTGATCTGTCGCTGAATCTAAAAGGCATCAT
CGCTCAACAATTGATCCCATCTCCGGATGGTAAAGAGCGCTGTTTAGCAGCGGAAGTTTTGCGCGACAGTCGATTAGTAC
AGGACTACATACGCGTAGGCGAGATCCATAAGCTCAAAGAAGTCATGCAAAACCCCAACGATCTTGGAATGACTACATTC
GATCAAAGCTTGGTCGAACTCTATCATAGCGGAGCCATTAACTATAAAGAGGCACTACGCCATGCTGATTCGCAGAACGA
GGTACGCCTGCGTATTAAACTCTCCGATGGAAAAGACGCCAAAACTCTGAGTCAAGGATTCGATGGAGTAGAAATTGCAG
AAATGTCATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-272)

3D structure

• AlphaFold DB: Q9PCX2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.58	97.606	0.601
pilU	Acinetobacter baylyi ADP1	59.887	94.149	0.564
pilU	Vibrio cholerae strain A1552	51.124	94.681	0.484
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.209	96.809	0.399