Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   NZ30_RS05450 Genome accession   NZ_CP009750
Coordinates   1233072..1234130 (+) Length   352 a.a.
NCBI ID   WP_003471536.1    Uniprot ID   A0A109HJ64
Organism   Xanthomonas translucens pv. undulosa strain ICMP11055     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1228072..1239130
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NZ30_RS05440 (NZ30_05325) - 1229587..1230234 (-) 648 WP_003471539.1 hypothetical protein -
  NZ30_RS05445 (NZ30_05330) - 1230426..1232855 (-) 2430 WP_003471537.1 penicillin-binding protein 1A -
  NZ30_RS05450 (NZ30_05335) pilM 1233072..1234130 (+) 1059 WP_003471536.1 pilus assembly protein PilM Machinery gene
  NZ30_RS05455 (NZ30_05340) - 1234130..1234867 (+) 738 WP_108084713.1 PilN domain-containing protein -
  NZ30_RS05460 (NZ30_05345) - 1234864..1235538 (+) 675 WP_003471530.1 type 4a pilus biogenesis protein PilO -
  NZ30_RS05465 (NZ30_05350) - 1235550..1236074 (+) 525 WP_170874096.1 pilus assembly protein PilP -
  NZ30_RS05470 (NZ30_05355) - 1236095..1237996 (+) 1902 WP_038239345.1 type IV pilus secretin PilQ family protein -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 37699.39 Da        Isoelectric Point: 4.4880

>NTDB_id=131842 NZ30_RS05450 WP_003471536.1 1233072..1234130(+) (pilM) [Xanthomonas translucens pv. undulosa strain ICMP11055]
MGLIPKSQQPLIGVDISSTAVKLLQLSRSGNRFRVEHYAVEPLPPNAVVEKNIVEVEAVGEAIRRAVTRSGTRARHAAAA
VAGSAVITKLIPMPAELDESELEAQVELEAVNYIPYPIEEVNLDFEVLGIIPNNPEMVQVLLAASRSENVELRQSALELG
GLIAKVMDVEAFAVENAFAMVASELPVAADGVVALVDIGATMTTLNVLRGGRSLYSREQVFGGKQLTDEVMRRYGLTYEE
AGMAKRQGGLPESYAIEVLEPFKEATVQQISRLLQFFYAGSEFNRVDHIVLAGGCAALAGLPEMVEEQLGVATVVANPLA
QMTLGPKVQAHALAQDAPALMIATGLALRSFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=131842 NZ30_RS05450 WP_003471536.1 1233072..1234130(+) (pilM) [Xanthomonas translucens pv. undulosa strain ICMP11055]
GTGGGGCTTATCCCAAAGAGTCAGCAACCGCTGATCGGTGTCGATATCAGTTCGACTGCGGTCAAACTGTTGCAGCTGTC
GCGTAGCGGCAATCGGTTCCGCGTAGAGCACTATGCCGTGGAACCCCTGCCGCCCAACGCCGTGGTCGAGAAGAACATCG
TCGAGGTCGAAGCCGTGGGCGAGGCGATCCGCCGCGCCGTGACCCGTTCCGGTACCCGCGCCAGGCATGCCGCGGCGGCG
GTGGCCGGCTCGGCGGTGATCACCAAGCTGATCCCGATGCCGGCCGAACTGGACGAGAGCGAACTGGAAGCGCAGGTCGA
GTTGGAGGCGGTCAACTACATCCCGTATCCGATCGAGGAAGTGAACCTGGACTTCGAGGTGCTGGGGATCATCCCCAACA
ATCCGGAGATGGTGCAGGTGCTGCTGGCCGCGTCGCGTTCGGAGAACGTCGAGCTGCGCCAGTCGGCGCTGGAGCTCGGC
GGCTTGATCGCCAAGGTCATGGACGTGGAGGCCTTCGCGGTCGAGAACGCGTTCGCGATGGTTGCCAGCGAGTTGCCGGT
GGCCGCCGACGGCGTGGTCGCCCTGGTCGATATCGGCGCCACCATGACCACGCTCAACGTGCTGCGCGGCGGGCGCAGCC
TGTACAGCCGCGAACAGGTGTTCGGCGGCAAGCAGCTGACCGACGAAGTGATGCGCCGCTACGGCCTGACCTACGAGGAA
GCCGGCATGGCCAAGCGCCAGGGCGGGCTGCCGGAAAGCTACGCGATCGAGGTGCTGGAGCCGTTCAAGGAGGCGACGGT
GCAGCAGATCAGCCGCCTGCTGCAGTTCTTCTACGCCGGCAGCGAATTCAACCGGGTCGACCACATCGTGCTGGCCGGCG
GTTGCGCGGCGCTGGCCGGGTTGCCGGAGATGGTCGAGGAACAACTGGGGGTGGCGACCGTGGTCGCCAACCCGCTGGCG
CAGATGACCCTGGGGCCGAAGGTCCAGGCGCATGCGCTGGCCCAGGACGCGCCGGCGCTGATGATCGCCACCGGCCTGGC
TCTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A109HJ64

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

51.136

100

0.511
  comM Acinetobacter nosocomialis M2

50.852

100

0.509
  comM Acinetobacter baylyi ADP1

50

99.432

0.497
  pilM Legionella pneumophila strain ERS1305867

46.286

99.432

0.46

Multiple sequence alignment