Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   NG74_RS11645 Genome accession   NZ_CP009679
Coordinates   2464254..2465324 (-) Length   356 a.a.
NCBI ID   WP_014418378.1    Uniprot ID   A0AAP4DGV9
Organism   Bacillus velezensis strain JS25R     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2459254..2470324
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NG74_RS11595 (NG74_02402) sipW 2459313..2459897 (-) 585 WP_022552967.1 signal peptidase I SipW -
  NG74_RS11600 (NG74_02403) tapA 2459869..2460540 (-) 672 WP_014418371.1 amyloid fiber anchoring/assembly protein TapA -
  NG74_RS11605 (NG74_02404) - 2460799..2461128 (+) 330 WP_012117979.1 DUF3889 domain-containing protein -
  NG74_RS11610 (NG74_02405) - 2461169..2461348 (-) 180 WP_022552966.1 YqzE family protein -
  NG74_RS11615 (NG74_02406) comGG 2461405..2461782 (-) 378 WP_022552965.1 competence type IV pilus minor pilin ComGG Machinery gene
  NG74_RS11620 (NG74_02407) comGF 2461783..2462178 (-) 396 WP_046559876.1 competence type IV pilus minor pilin ComGF -
  NG74_RS11625 (NG74_02408) comGE 2462192..2462506 (-) 315 WP_021494312.1 competence type IV pilus minor pilin ComGE Machinery gene
  NG74_RS11630 (NG74_02409) comGD 2462490..2462927 (-) 438 WP_007612572.1 competence type IV pilus minor pilin ComGD Machinery gene
  NG74_RS11635 (NG74_02410) comGC 2462917..2463225 (-) 309 WP_014418376.1 competence type IV pilus major pilin ComGC Machinery gene
  NG74_RS11640 (NG74_02411) comGB 2463230..2464267 (-) 1038 WP_022552962.1 competence type IV pilus assembly protein ComGB Machinery gene
  NG74_RS11645 (NG74_02412) comGA 2464254..2465324 (-) 1071 WP_014418378.1 competence type IV pilus ATPase ComGA Machinery gene
  NG74_RS11650 (NG74_02413) - 2465517..2466467 (-) 951 WP_014418379.1 magnesium transporter CorA family protein -
  NG74_RS11655 (NG74_02414) - 2466613..2467914 (+) 1302 WP_022552961.1 hemolysin family protein -
  NG74_RS11660 (NG74_02415) - 2467952..2468782 (-) 831 WP_022552960.1 STAS domain-containing protein -
  NG74_RS11670 (NG74_02417) - 2468989..2469369 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  NG74_RS11675 (NG74_02418) - 2469606..2469851 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40529.81 Da        Isoelectric Point: 9.3485

>NTDB_id=131085 NG74_RS11645 WP_014418378.1 2464254..2465324(-) (comGA) [Bacillus velezensis strain JS25R]
MKNIETYSRNIINEAYTARASDIHIVPRERDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKQQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=131085 NG74_RS11645 WP_014418378.1 2464254..2465324(-) (comGA) [Bacillus velezensis strain JS25R]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAGGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGTG
TAAGGCTTATTTCTCATTTCAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGAAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACAGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCAGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCAGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCGACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAACGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGCTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCAATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTTGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.303

100

0.823
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374

Multiple sequence alignment