Detailed information    

insolico Bioinformatically predicted

Overview

Name   recU   Type   Machinery gene
Locus tag   BF32_RS23985 Genome accession   NZ_CP009600
Coordinates   4538343..4538945 (-) Length   200 a.a.
NCBI ID   WP_000155593.1    Uniprot ID   A0A2A7DG84
Organism   Bacillus thuringiensis strain HD571     
Function   plasmid transformation; homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4533343..4543945
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BF32_RS23965 (BF32_4688) dnaD 4533668..4534375 (+) 708 WP_000728549.1 DNA replication protein DnaD -
  BF32_RS23970 (BF32_4689) nth 4534391..4535038 (+) 648 WP_000933022.1 endonuclease III -
  BF32_RS23975 (BF32_4690) - 4535042..4535560 (+) 519 WP_000442670.1 YpoC family protein -
  BF32_RS23980 (BF32_4691) - 4535584..4538277 (-) 2694 WP_001283115.1 PBP1A family penicillin-binding protein -
  BF32_RS23985 (BF32_4692) recU 4538343..4538945 (-) 603 WP_000155593.1 Holliday junction resolvase RecU Machinery gene
  BF32_RS23990 (BF32_4693) - 4539049..4540074 (+) 1026 WP_042329104.1 DUF2515 domain-containing protein -
  BF32_RS29730 - 4540341..4540517 (-) 177 WP_001982922.1 hypothetical protein -
  BF32_RS23995 (BF32_4695) - 4540603..4540845 (+) 243 WP_001252043.1 hypothetical protein -
  BF32_RS24000 (BF32_4696) - 4540861..4541205 (+) 345 WP_000872959.1 YppE family protein -
  BF32_RS24005 (BF32_4697) - 4541231..4541422 (-) 192 WP_000242663.1 YppF family protein -
  BF32_RS24010 (BF32_4698) - 4541600..4542079 (+) 480 WP_000487704.1 YppG family protein -
  BF32_RS24015 (BF32_4699) - 4542234..4542662 (+) 429 WP_000546294.1 CotD family spore coat protein -
  BF32_RS24020 (BF32_4700) - 4542719..4543273 (+) 555 WP_000862921.1 DUF1273 domain-containing protein -
  BF32_RS24025 (BF32_4701) gpsB 4543371..4543709 (+) 339 WP_000622430.1 cell division regulator GpsB -

Sequence

Protein


Download         Length: 200 a.a.        Molecular weight: 23312.63 Da        Isoelectric Point: 9.5601

>NTDB_id=130586 BF32_RS23985 WP_000155593.1 4538343..4538945(-) (recU) [Bacillus thuringiensis strain HD571]
MTIRYPNGKRYNQASQPHKTPIKKHTYSNRGMSLEEELNETNEYYLTHNIACVHKKPTPLQIVKVDYPARSAAVVKEAYF
KQPSTTDYNGVYKGKYIDFEAKETKNKTSFPLQNFHLHQIEHMKQVIAHNGIAFVIIKFTLFDELYLLDAKHIIAFWNRQ
NTGGRKSITKEEIVEHGSLLSCGYHPRIDYIRVLDTVYFS

Nucleotide


Download         Length: 603 bp        

>NTDB_id=130586 BF32_RS23985 WP_000155593.1 4538343..4538945(-) (recU) [Bacillus thuringiensis strain HD571]
ATGACCATTCGTTACCCAAATGGAAAACGGTACAATCAAGCTTCACAACCTCATAAAACACCAATAAAAAAACATACTTA
TAGTAACAGAGGTATGTCCCTTGAAGAGGAATTGAATGAAACAAATGAATATTACTTAACCCATAATATTGCATGTGTAC
ATAAAAAACCTACACCTCTTCAAATTGTAAAAGTAGATTACCCCGCTCGAAGTGCTGCAGTGGTAAAAGAAGCGTATTTT
AAACAACCTTCTACAACAGATTACAACGGTGTATATAAAGGGAAATACATCGATTTTGAAGCGAAAGAAACAAAAAATAA
AACCAGCTTTCCACTTCAAAACTTCCACCTTCATCAAATTGAACATATGAAGCAAGTAATTGCTCATAATGGAATTGCAT
TTGTTATTATTAAATTTACACTTTTTGATGAACTTTATTTATTAGATGCAAAACATATTATTGCATTTTGGAATCGTCAA
AATACAGGTGGACGAAAATCGATTACGAAAGAAGAAATAGTAGAACATGGATCTTTATTATCATGCGGTTATCACCCTCG
GATTGATTATATCCGTGTACTAGACACGGTTTATTTTTCGTGA

Domains

Predicted by InterproScan.

Recombination protein U (RecU)

(30-195)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2A7DG84

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recU Bacillus subtilis subsp. subtilis str. 168

62.5

100

0.625

Multiple sequence alignment