Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   PA50071_RS26305 Genome accession   NZ_CP012001
Coordinates   5727609..5728232 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa DSM 50071 = NBRC 12689 strain DSM 50071     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5722609..5733232
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PA50071_RS26285 (PA50071_26295) aroB 5723204..5724310 (-) 1107 WP_003107839.1 3-dehydroquinate synthase -
  PA50071_RS26290 (PA50071_26300) aroK 5724360..5724878 (-) 519 WP_003095833.1 shikimate kinase AroK -
  PA50071_RS26295 (PA50071_26305) pilQ 5724890..5727034 (-) 2145 WP_012614629.1 type 4a pilus secretin PilQ Machinery gene
  PA50071_RS26300 (PA50071_26310) comL/pilP 5727088..5727612 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  PA50071_RS26305 (PA50071_26315) pilO 5727609..5728232 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  PA50071_RS26310 (PA50071_26320) pilN 5728229..5728825 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  PA50071_RS26315 (PA50071_26325) pilM 5728825..5729889 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  PA50071_RS26320 (PA50071_26330) - 5730074..5732545 (+) 2472 WP_003103276.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=130084 PA50071_RS26305 WP_003103268.1 5727609..5728232(-) (pilO) [Pseudomonas aeruginosa DSM 50071 = NBRC 12689 strain DSM 50071]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=130084 PA50071_RS26305 WP_003103268.1 5727609..5728232(-) (pilO) [Pseudomonas aeruginosa DSM 50071 = NBRC 12689 strain DSM 50071]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGTGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTTGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACTTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401