Detailed information NTG0127674

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: IX88_RS06390
• Genome accession: NZ_CP009256
• Coordinates: 1339424..1340542 (-)
• Length: 372 a.a.
• NCBI ID: WP_038405476.1
• Uniprot ID: -
• Organism: Acinetobacter baumannii strain AB31
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1334424..1345542

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IX88_RS06360 (IX88_06425)	-	1335253..1336101 (+)	849	WP_038405475.1	class II glutamine amidotransferase	-
IX88_RS06365 (IX88_06430)	-	1336354..1336809 (+)	456	WP_000782976.1	bacteriohemerythrin	-
IX88_RS06370 (IX88_06435)	-	1336962..1338029 (+)	1068	WP_038405809.1	membrane protein	-
IX88_RS06375 (IX88_06440)	-	1338029..1338352 (+)	324	WP_000442585.1	RnfH family protein	-
IX88_RS06380 (IX88_06445)	-	1338407..1338805 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
IX88_RS06385 (IX88_06450)	fur	1338917..1339354 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
IX88_RS06390 (IX88_06455)	pilU	1339424..1340542 (-)	1119	WP_038405476.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
IX88_RS06395 (IX88_06460)	pilT	1340570..1341607 (-)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
IX88_RS06400 (IX88_06465)	-	1341734..1342426 (+)	693	WP_038405477.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41917.17 Da
• Isoelectric Point: 6.6944

>NTDB_id=127674 IX88_RS06390 WP_038405476.1 1339424..1340542(-) (pilU) [Acinetobacter baumannii strain AB31]
MDFNDLLNLMVEKKSSDLFIIDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=127674 IX88_RS06390 WP_038405476.1 1339424..1340542(-) (pilU) [Acinetobacter baumannii strain AB31]
ATGGATTTTAATGACCTGCTCAACCTGATGGTTGAAAAGAAATCTTCCGATCTCTTTATTATAGATGGCGTTGCGCCCTC
TATGAAGATTAATGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCA
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCACCAGATGTCATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTTGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCACAGCCAGCTTTACATGGATTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (30-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	69.022	98.925	0.683
pilU	Vibrio cholerae strain A1552	52.973	99.462	0.527
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392