Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   ASU1_RS06230 Genome accession   NZ_CP009159
Coordinates   1366682..1367977 (-) Length   431 a.a.
NCBI ID   WP_014991934.1    Uniprot ID   K0G4S2
Organism   Actinobacillus suis ATCC 33415     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1368384..1382435 1366682..1367977 flank 407


Gene organization within MGE regions


Location: 1366682..1382435
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ASU1_RS06230 (ASU1_06180) comE 1366682..1367977 (-) 1296 WP_014991934.1 type IV pilus secretin PilQ Machinery gene
  ASU1_RS06235 (ASU1_06185) - 1367987..1368391 (-) 405 WP_014991935.1 hypothetical protein -
  ASU1_RS06240 (ASU1_06190) - 1368384..1368911 (-) 528 WP_014991936.1 hypothetical protein -
  ASU1_RS06245 (ASU1_06195) - 1368914..1369435 (-) 522 WP_014991937.1 hypothetical protein -
  ASU1_RS06250 (ASU1_06200) - 1369417..1370103 (-) 687 WP_014991938.1 hypothetical protein -
  ASU1_RS06255 (ASU1_06205) - 1370242..1372827 (+) 2586 WP_014991939.1 penicillin-binding protein 1A -
  ASU1_RS06260 (ASU1_06210) aroK 1373091..1373612 (+) 522 WP_005623982.1 shikimate kinase AroK -
  ASU1_RS06265 (ASU1_06215) aroB 1373629..1374717 (+) 1089 WP_014991940.1 3-dehydroquinate synthase -
  ASU1_RS06270 (ASU1_06220) - 1374721..1375557 (+) 837 WP_014991941.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  ASU1_RS06275 (ASU1_06225) - 1375627..1376985 (+) 1359 WP_014991942.1 sodium-dependent transporter -
  ASU1_RS06280 (ASU1_06230) pgi 1377113..1378753 (-) 1641 WP_014991943.1 glucose-6-phosphate isomerase -
  ASU1_RS06285 (ASU1_06235) - 1378792..1379043 (-) 252 WP_014991944.1 accessory factor UbiK family protein -
  ASU1_RS06310 (ASU1_06240) aroE 1379760..1380584 (-) 825 WP_014991945.1 shikimate dehydrogenase -
  ASU1_RS06315 (ASU1_06245) - 1380590..1381144 (-) 555 WP_014991946.1 Sua5/YciO/YrdC/YwlC family protein -
  ASU1_RS06320 (ASU1_06250) - 1381137..1381670 (-) 534 WP_014991947.1 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
  ASU1_RS06325 (ASU1_06255) yqfB 1381670..1381975 (-) 306 WP_014991948.1 N(4)-acetylcytidine aminohydrolase -
  ASU1_RS06330 (ASU1_06260) recX 1381968..1382423 (-) 456 WP_014991949.1 recombination regulator RecX -

Sequence

Protein


Download         Length: 431 a.a.        Molecular weight: 47947.13 Da        Isoelectric Point: 9.4585

>NTDB_id=127154 ASU1_RS06230 WP_014991934.1 1366682..1367977(-) (comE) [Actinobacillus suis ATCC 33415]
MKRMILLLFFILPPVIAHAFSISLKNAPTAKILSYLAEEHGKNIVLSDNIETNTTLRIENSNFDSVLKSITRANKLTSAY
ENQIYFIGHKKDDKDPNRGLNLELLKPKLITKTIKLDYAKAAEVIESLTKGSGNFLSENGYLHFDDRSNSLIIKDSPESM
KNIVKLIRNLDRPTEQIAIEARIVTISSENLQELGVRWGMFSPANGHHKIAGSLEANGLPNTNHLNVNFPVNNAASLALQ
VAKINGRVLDLELTALEQENDVEIIASPRLLTTNKKPASIKQGTEIPYVLYNRKDEVKNIEFKEAVLGLQVTPHISNDNQ
ILLDLVVTQNSPNSTSSTVHGLVTIDKQELNTQVFAQHGETIVLGGIFQHLTAKGEDRVPILGSIPVLKKLFSHSSDRIS
KRELVIFVTPYIIKSEKQQISSHSLQKLPPK

Nucleotide


Download         Length: 1296 bp        

>NTDB_id=127154 ASU1_RS06230 WP_014991934.1 1366682..1367977(-) (comE) [Actinobacillus suis ATCC 33415]
ATGAAAAGAATGATTTTGCTACTCTTTTTTATATTGCCCCCTGTGATTGCACATGCGTTTTCTATTTCATTAAAAAATGC
GCCGACCGCAAAAATTCTGAGTTATTTAGCGGAAGAGCACGGAAAAAATATTGTGCTAAGCGACAATATTGAAACGAATA
CCACATTAAGAATTGAAAATAGCAATTTTGATAGTGTGTTAAAAAGTATTACTCGAGCAAATAAGTTGACCAGTGCGTAT
GAAAACCAAATCTATTTTATTGGTCATAAAAAAGACGATAAGGATCCTAACAGGGGATTAAATCTGGAATTATTAAAACC
TAAACTGATTACTAAAACCATTAAGTTAGATTATGCTAAAGCAGCGGAAGTAATTGAATCTTTAACTAAAGGTAGCGGTA
ATTTTTTATCGGAAAACGGCTATTTACATTTTGATGATCGCAGTAATAGTTTGATTATTAAAGATAGCCCGGAATCAATG
AAAAATATCGTGAAATTAATCAGAAATTTGGATAGACCGACCGAACAGATTGCAATTGAAGCGAGAATAGTCACAATCAG
TAGTGAGAATTTACAAGAGCTTGGCGTGCGTTGGGGAATGTTTTCTCCTGCAAACGGACATCATAAAATTGCCGGTTCGC
TTGAAGCAAATGGGCTACCGAATACTAATCATTTAAACGTCAATTTTCCGGTAAATAATGCTGCTTCACTTGCGCTACAA
GTGGCAAAAATTAATGGGCGAGTGCTTGATTTGGAACTTACCGCTTTGGAACAAGAAAATGATGTAGAAATTATTGCCAG
CCCACGTTTACTGACCACTAATAAGAAACCGGCAAGTATCAAACAAGGGACTGAAATTCCTTATGTGCTTTATAACCGTA
AAGATGAAGTGAAAAATATCGAATTTAAAGAAGCCGTTTTAGGGCTACAGGTTACACCGCATATTTCAAACGATAATCAA
ATTTTGCTTGATCTGGTGGTGACACAAAATTCACCAAATTCAACCAGTTCAACGGTACACGGTTTAGTGACGATTGATAA
GCAGGAACTGAATACTCAGGTATTTGCTCAACACGGTGAGACTATTGTACTTGGTGGTATTTTTCAGCATTTAACTGCAA
AAGGTGAGGATAGAGTCCCAATTTTAGGTTCAATTCCAGTACTTAAAAAATTATTTAGCCATTCCAGTGATAGGATCAGT
AAGCGTGAATTAGTTATTTTCGTTACACCTTATATTATCAAAAGCGAAAAACAGCAAATTTCCTCGCATTCTCTACAGAA
ATTACCGCCAAAATAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(255-414)

Bacterial type II/III secretion system short domain (Secretin_N)

(111-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K0G4S2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Glaesserella parasuis strain SC1401

66.587

96.52

0.643
  comE Haemophilus influenzae Rd KW20

52.817

98.84

0.522
  comE Haemophilus influenzae 86-028NP

52.582

98.84

0.52
  pilQ Vibrio campbellii strain DS40M4

42.08

98.144

0.413
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

42.754

96.056

0.411
  pilQ Vibrio cholerae strain A1552

42.754

96.056

0.411

Multiple sequence alignment