Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   GZ59_RS04405 Genome accession   NZ_CP009125
Coordinates   987707..988255 (+) Length   182 a.a.
NCBI ID   WP_039290400.1    Uniprot ID   M4GYR9
Organism   Pectobacterium atrosepticum strain 21A     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 958887..987642 987707..988255 flank 65


Gene organization within MGE regions


Location: 958887..988255
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GZ59_RS04255 (GZ59_08830) - 958887..959660 (+) 774 WP_039290380.1 winged helix-turn-helix domain-containing protein -
  GZ59_RS04260 (GZ59_08840) - 959776..960117 (+) 342 WP_226302337.1 FidL -
  GZ59_RS04265 (GZ59_08850) - 960179..960751 (-) 573 WP_011092548.1 TetR/AcrR family transcriptional regulator -
  GZ59_RS04270 (GZ59_08860) - 960844..961737 (+) 894 WP_011092549.1 ABC transporter six-transmembrane domain-containing protein -
  GZ59_RS04275 (GZ59_08870) cueP 961843..962379 (-) 537 WP_373365831.1 copper-binding periplasmic metallochaperone CueP -
  GZ59_RS04280 (GZ59_08880) - 962631..963395 (-) 765 WP_011092551.1 sulfite exporter TauE/SafE family protein -
  GZ59_RS04285 (GZ59_08890) - 963711..964310 (-) 600 WP_011092552.1 TetR/AcrR family transcriptional regulator -
  GZ59_RS04290 (GZ59_08900) - 964428..965015 (+) 588 WP_011092553.1 NAD(P)H-dependent oxidoreductase -
  GZ59_RS04295 (GZ59_08910) - 966072..966578 (-) 507 WP_011092556.1 GNAT family N-acetyltransferase -
  GZ59_RS04300 (GZ59_08920) - 966776..967675 (-) 900 WP_011092557.1 LysR family transcriptional regulator -
  GZ59_RS04305 (GZ59_08930) - 967774..968301 (+) 528 WP_011092558.1 GNAT family N-acetyltransferase -
  GZ59_RS04310 (GZ59_08940) - 968298..969485 (+) 1188 WP_011092559.1 MFS transporter -
  GZ59_RS23810 - 969483..969653 (-) 171 Protein_845 XRE family transcriptional regulator -
  GZ59_RS22615 - 969679..969804 (+) 126 Protein_846 helix-turn-helix domain-containing protein -
  GZ59_RS04315 (GZ59_08960) - 970002..970295 (+) 294 WP_011092560.1 DUF1778 domain-containing protein -
  GZ59_RS04320 (GZ59_08970) - 970292..970782 (+) 491 Protein_848 GNAT family N-acetyltransferase -
  GZ59_RS04325 (GZ59_08980) - 971136..973742 (+) 2607 WP_011092562.1 AAA family ATPase -
  GZ59_RS04335 (GZ59_08990) ccdA 974187..974405 (-) 219 WP_011092563.1 type II toxin-antitoxin system antitoxin CcdA -
  GZ59_RS04340 (GZ59_09000) - 974420..974668 (-) 249 WP_011092564.1 helix-turn-helix domain-containing protein -
  GZ59_RS04345 (GZ59_09010) - 974753..975007 (-) 255 WP_011092565.1 helix-turn-helix domain-containing protein -
  GZ59_RS22620 (GZ59_09020) - 975111..975353 (+) 243 WP_011092566.1 helix-turn-helix domain-containing protein -
  GZ59_RS04350 (GZ59_09030) - 975531..976418 (+) 888 WP_011092136.1 ParA family protein -
  GZ59_RS22405 (GZ59_09040) - 976432..976869 (-) 438 WP_051122321.1 hypothetical protein -
  GZ59_RS04360 (GZ59_09050) - 977097..977489 (+) 393 WP_011092138.1 hypothetical protein -
  GZ59_RS04365 (GZ59_09060) dnaB-PI 977482..978849 (+) 1368 WP_048018345.1 SPI-7-type island replicative DNA helicase -
  GZ59_RS04370 (GZ59_09070) - 978849..980573 (+) 1725 WP_039290390.1 ParB family protein -
  GZ59_RS04375 (GZ59_09080) - 980577..980849 (+) 273 WP_011092141.1 hypothetical protein -
  GZ59_RS04380 (GZ59_09090) - 980874..981488 (+) 615 WP_011092142.1 DUF2857 domain-containing protein -
  GZ59_RS23815 (GZ59_09100) - 981497..981751 (+) 255 WP_011092143.1 hypothetical protein -
  GZ59_RS04385 (GZ59_09110) - 981924..983120 (+) 1197 WP_011092144.1 STY4528 family pathogenicity island replication protein -
  GZ59_RS04390 (GZ59_09120) - 983490..984287 (+) 798 WP_039290394.1 PFL_4669 family integrating conjugative element protein -
  GZ59_RS04395 (GZ59_09130) - 984287..986302 (+) 2016 WP_039290396.1 DNA topoisomerase III -
  GZ59_RS04400 (GZ59_09150) - 987151..987642 (+) 492 WP_011092149.1 STY4534 family ICE replication protein -
  GZ59_RS04405 (GZ59_09160) ssb 987707..988255 (+) 549 WP_039290400.1 single-stranded DNA-binding protein Machinery gene

Sequence


Protein


Download         Length: 182 a.a.        Molecular weight: 19684.06 Da        Isoelectric Point: 8.0013

>NTDB_id=126982 GZ59_RS04405 WP_039290400.1 987707..988255(+) (ssb) [Pectobacterium atrosepticum strain 21A]
MASRGVNKVILVGHLGQDPEIRYMPNGGAVANMTLATSETWRDKQSGEQKEKTEWHRVVIFGKLAEVAGEYLRKGSQVYI
EGSLQTRKWADQAGVERYTTEVVVNVGGTLQMLGSRPNAGSNNQSAASTAWGKPQQPVAPAQSGPPQSQRSAGNEPPMDF
DDDIPFLGFGYGVCRKAIYAVS

Nucleotide


Download         Length: 549 bp        

>NTDB_id=126982 GZ59_RS04405 WP_039290400.1 987707..988255(+) (ssb) [Pectobacterium atrosepticum strain 21A]
ATGGCTTCACGCGGTGTTAACAAAGTTATCTTAGTTGGCCATCTCGGTCAGGATCCTGAAATTCGCTATATGCCGAATGG
TGGTGCAGTTGCCAACATGACATTAGCGACCTCTGAAACCTGGCGGGATAAGCAAAGTGGTGAACAGAAAGAGAAGACCG
AATGGCACCGTGTGGTGATTTTCGGGAAACTCGCTGAAGTCGCTGGCGAATATCTTCGCAAAGGCTCTCAGGTTTACATT
GAAGGTTCATTACAAACGCGTAAATGGGCTGATCAGGCTGGGGTAGAACGCTATACGACTGAAGTTGTCGTTAACGTTGG
CGGCACATTGCAGATGCTAGGCTCTCGTCCTAACGCTGGAAGTAACAACCAAAGTGCAGCCAGTACTGCATGGGGAAAAC
CTCAGCAGCCAGTTGCACCGGCGCAGAGTGGTCCCCCTCAGTCTCAACGTTCAGCAGGCAATGAACCGCCGATGGATTTT
GACGATGATATCCCGTTCCTTGGGTTTGGTTATGGCGTATGTCGCAAGGCAATCTACGCCGTTTCGTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB M4GYR9

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Vibrio cholerae strain A1552

67.797

97.253

0.659

  ssb Glaesserella parasuis strain SC1401

51.381

99.451

0.511

  ssb Neisseria meningitidis MC58

44.318

96.703

0.429

  ssb Neisseria gonorrhoeae MS11

44.318

96.703

0.429


Multiple sequence alignment