Detailed information NTG0125258

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: HV96_RS02075
• Genome accession: NZ_CP008871
• Coordinates: 437009..438157 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain W45909
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 432009..443157

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HV96_RS02050 (HV96_31860)	-	433160..433456 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
HV96_RS02055 (HV96_25915)	-	433453..434046 (-)	594	WP_003084544.1	YggT family protein	-
HV96_RS02060 (HV96_25910)	proC	434057..434878 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
HV96_RS02065 (HV96_25905)	-	434890..435582 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
HV96_RS02070 (HV96_25900)	pilT	435797..436831 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
HV96_RS02075 (HV96_25895)	pilU	437009..438157 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
HV96_RS02080 (HV96_25890)	czcD	438165..439064 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
HV96_RS02085 (HV96_25885)	-	439218..439622 (+)	405	WP_003084560.1	NINE protein	-
HV96_RS02090 (HV96_25880)	-	439865..441238 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
HV96_RS02095 (HV96_25875)	-	441235..442419 (+)	1185	WP_023114752.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=125258 HV96_RS02075 WP_003100959.1 437009..438157(+) (pilU) [Pseudomonas aeruginosa strain W45909]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=125258 HV96_RS02075 WP_003100959.1 437009..438157(+) (pilU) [Pseudomonas aeruginosa strain W45909]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTTGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382