Detailed information NTG0125225

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: HV97_RS02535
• Genome accession: NZ_CP008870
• Coordinates: 544913..546061 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain W36662
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 539913..551061

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HV97_RS02510 (HV97_31875)	-	541064..541360 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
HV97_RS02515 (HV97_30985)	-	541357..541950 (-)	594	WP_003084544.1	YggT family protein	-
HV97_RS02520 (HV97_30990)	proC	541961..542782 (-)	822	WP_003142635.1	pyrroline-5-carboxylate reductase	-
HV97_RS02525 (HV97_30995)	-	542794..543486 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
HV97_RS02530 (HV97_31000)	pilT	543701..544735 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
HV97_RS02535 (HV97_31005)	pilU	544913..546061 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
HV97_RS02540 (HV97_31010)	czcD	546069..546968 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
HV97_RS02545 (HV97_31015)	-	547122..547526 (+)	405	WP_003084560.1	NINE protein	-
HV97_RS02550 (HV97_31020)	-	547769..549142 (+)	1374	WP_015502220.1	pyridoxal-phosphate dependent enzyme	-
HV97_RS02555 (HV97_31025)	-	549139..550323 (+)	1185	WP_023086800.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=125225 HV97_RS02535 WP_003100959.1 544913..546061(+) (pilU) [Pseudomonas aeruginosa strain W36662]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=125225 HV97_RS02535 WP_003100959.1 544913..546061(+) (pilU) [Pseudomonas aeruginosa strain W36662]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGTCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382