Detailed information NTG0125190

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: HV98_RS02080
• Genome accession: NZ_CP008869
• Coordinates: 433094..434242 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain W16407
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 428094..439242

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HV98_RS02055 (HV98_31875)	-	429245..429541 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
HV98_RS02060 (HV98_26405)	-	429538..430131 (-)	594	WP_003084544.1	YggT family protein	-
HV98_RS02065 (HV98_26400)	proC	430142..430963 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
HV98_RS02070 (HV98_26395)	-	430975..431667 (-)	693	WP_010793543.1	YggS family pyridoxal phosphate-dependent enzyme	-
HV98_RS02075 (HV98_26390)	pilT	431882..432916 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
HV98_RS02080 (HV98_26385)	pilU	433094..434242 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
HV98_RS02085 (HV98_26380)	czcD	434250..435149 (-)	900	WP_059303348.1	CDF family zinc transporter CzcD	-
HV98_RS02090 (HV98_26375)	-	435303..435707 (+)	405	WP_003084560.1	NINE protein	-
HV98_RS02095 (HV98_26370)	-	435950..437323 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
HV98_RS02100 (HV98_26365)	-	437320..438504 (+)	1185	WP_003100953.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=125190 HV98_RS02080 WP_003100959.1 433094..434242(+) (pilU) [Pseudomonas aeruginosa strain W16407]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=125190 HV98_RS02080 WP_003100959.1 433094..434242(+) (pilU) [Pseudomonas aeruginosa strain W16407]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382