Detailed information NTG0125171

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: HV99_RS09605
• Genome accession: NZ_CP008868
• Coordinates: 2095194..2096342 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain T63266
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2090194..2101342

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HV99_RS09580	-	2091345..2091641 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
HV99_RS09585 (HV99_09580)	-	2091638..2092231 (-)	594	WP_003084544.1	YggT family protein	-
HV99_RS09590 (HV99_09585)	proC	2092242..2093063 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
HV99_RS09595 (HV99_09590)	-	2093075..2093767 (-)	693	WP_031643148.1	YggS family pyridoxal phosphate-dependent enzyme	-
HV99_RS09600 (HV99_09595)	pilT	2093982..2095016 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
HV99_RS09605 (HV99_09600)	pilU	2095194..2096342 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
HV99_RS09610 (HV99_09605)	czcD	2096350..2097249 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
HV99_RS09615 (HV99_09610)	-	2097403..2097807 (+)	405	WP_003084560.1	NINE protein	-
HV99_RS09620 (HV99_09615)	-	2098050..2099423 (+)	1374	WP_034020583.1	pyridoxal-phosphate dependent enzyme	-
HV99_RS09625 (HV99_09620)	-	2099420..2100604 (+)	1185	WP_003117897.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=125171 HV99_RS09605 WP_003100959.1 2095194..2096342(+) (pilU) [Pseudomonas aeruginosa strain T63266]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=125171 HV99_RS09605 WP_003100959.1 2095194..2096342(+) (pilU) [Pseudomonas aeruginosa strain T63266]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382