Detailed information NTG0125136

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: HW00_RS13470
• Genome accession: NZ_CP008867
• Coordinates: 2926758..2927906 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain T52373
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2921758..2932906

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HW00_RS13445	-	2922909..2923205 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
HW00_RS13450 (HW00_13475)	-	2923202..2923795 (-)	594	WP_003084544.1	YggT family protein	-
HW00_RS13455 (HW00_13480)	proC	2923806..2924627 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
HW00_RS13460 (HW00_13485)	-	2924639..2925331 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
HW00_RS13465 (HW00_13490)	pilT	2925546..2926580 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
HW00_RS13470 (HW00_13495)	pilU	2926758..2927906 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
HW00_RS13475 (HW00_13500)	czcD	2927914..2928813 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
HW00_RS13480 (HW00_13505)	-	2928968..2929372 (+)	405	WP_003084560.1	NINE protein	-
HW00_RS13485 (HW00_13510)	-	2929615..2930988 (+)	1374	WP_004365040.1	pyridoxal-phosphate dependent enzyme	-
HW00_RS13490 (HW00_13515)	-	2930985..2932169 (+)	1185	WP_025324561.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=125136 HW00_RS13470 WP_003100959.1 2926758..2927906(+) (pilU) [Pseudomonas aeruginosa strain T52373]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=125136 HW00_RS13470 WP_003100959.1 2926758..2927906(+) (pilU) [Pseudomonas aeruginosa strain T52373]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGTCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382