Detailed information NTG0125085

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: HW01_RS02175
• Genome accession: NZ_CP008866
• Coordinates: 450377..451525 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain T38079
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 445377..456525

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HW01_RS02150 (HW01_31675)	-	446528..446824 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
HW01_RS02155 (HW01_13925)	-	446821..447414 (-)	594	WP_003084544.1	YggT family protein	-
HW01_RS02160 (HW01_13920)	proC	447425..448246 (-)	822	WP_003142635.1	pyrroline-5-carboxylate reductase	-
HW01_RS02165 (HW01_13915)	-	448258..448950 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
HW01_RS02170 (HW01_13910)	pilT	449165..450199 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
HW01_RS02175 (HW01_13905)	pilU	450377..451525 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
HW01_RS02180 (HW01_13900)	czcD	451533..452432 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
HW01_RS02185 (HW01_13895)	-	452587..452991 (+)	405	WP_003084560.1	NINE protein	-
HW01_RS02190 (HW01_13890)	-	453234..454607 (+)	1374	WP_003459266.1	pyridoxal-phosphate dependent enzyme	-
HW01_RS02195 (HW01_13885)	-	454604..455788 (+)	1185	WP_016852373.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=125085 HW01_RS02175 WP_003100959.1 450377..451525(+) (pilU) [Pseudomonas aeruginosa strain T38079]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=125085 HW01_RS02175 WP_003100959.1 450377..451525(+) (pilU) [Pseudomonas aeruginosa strain T38079]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382