Detailed information NTG0125018

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: HV95_RS02130
• Genome accession: NZ_CP008864
• Coordinates: 447307..448455 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain W60856
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 442307..453455

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HV95_RS02105 (HV95_32275)	-	443458..443754 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
HV95_RS02110 (HV95_27900)	-	443751..444344 (-)	594	WP_003084544.1	YggT family protein	-
HV95_RS02115 (HV95_27905)	proC	444355..445176 (-)	822	WP_003142635.1	pyrroline-5-carboxylate reductase	-
HV95_RS02120 (HV95_27910)	-	445188..445880 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
HV95_RS02125 (HV95_27915)	pilT	446095..447129 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
HV95_RS02130 (HV95_27920)	pilU	447307..448455 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
HV95_RS02135 (HV95_27925)	czcD	448463..449362 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
HV95_RS02140 (HV95_27930)	-	449517..449921 (+)	405	WP_003084560.1	NINE protein	-
HV95_RS02145 (HV95_27935)	-	450164..451537 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
HV95_RS02150 (HV95_27940)	-	451534..452718 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=125018 HV95_RS02130 WP_003100959.1 447307..448455(+) (pilU) [Pseudomonas aeruginosa strain W60856]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=125018 HV95_RS02130 WP_003100959.1 447307..448455(+) (pilU) [Pseudomonas aeruginosa strain W60856]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGTCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382