Detailed information NTG0125001

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: HW03_RS18655
• Genome accession: NZ_CP008863
• Coordinates: 4042587..4043735 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain M37351
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4037587..4048735

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HW03_RS18630	-	4038738..4039034 (-)	297	WP_031632852.1	DUF167 domain-containing protein	-
HW03_RS18635 (HW03_18830)	-	4039031..4039624 (-)	594	WP_003084544.1	YggT family protein	-
HW03_RS18640 (HW03_18835)	proC	4039635..4040456 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
HW03_RS18645 (HW03_18840)	-	4040468..4041160 (-)	693	WP_004365038.1	YggS family pyridoxal phosphate-dependent enzyme	-
HW03_RS18650 (HW03_18845)	pilT	4041375..4042409 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
HW03_RS18655 (HW03_18850)	pilU	4042587..4043735 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
HW03_RS18660 (HW03_18855)	czcD	4043743..4044642 (-)	900	WP_004365039.1	CDF family zinc transporter CzcD	-
HW03_RS18665 (HW03_18860)	-	4044797..4045201 (+)	405	WP_003084560.1	NINE protein	-
HW03_RS18670 (HW03_18865)	-	4045444..4046817 (+)	1374	WP_004365040.1	pyridoxal-phosphate dependent enzyme	-
HW03_RS18675 (HW03_18870)	-	4046814..4047998 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=125001 HW03_RS18655 WP_003110016.1 4042587..4043735(+) (pilU) [Pseudomonas aeruginosa strain M37351]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=125001 HW03_RS18655 WP_003110016.1 4042587..4043735(+) (pilU) [Pseudomonas aeruginosa strain M37351]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTATATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCATG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382