Detailed information NTG0124962

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: HW05_RS15150
• Genome accession: NZ_CP008861
• Coordinates: 3289824..3290972 (-)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain H47921
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3284824..3295972

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HW05_RS15130 (HW05_15255)	-	3285562..3286746 (-)	1185	WP_003100953.1	cystathionine gamma-synthase	-
HW05_RS15135 (HW05_15260)	-	3286743..3288116 (-)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
HW05_RS15140 (HW05_15265)	-	3288359..3288763 (-)	405	WP_003084560.1	NINE protein	-
HW05_RS15145 (HW05_15270)	czcD	3288917..3289816 (+)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
HW05_RS15150 (HW05_15275)	pilU	3289824..3290972 (-)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
HW05_RS15155 (HW05_15280)	pilT	3291150..3292184 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
HW05_RS15160 (HW05_15285)	-	3292399..3293091 (+)	693	WP_010793543.1	YggS family pyridoxal phosphate-dependent enzyme	-
HW05_RS15165 (HW05_15290)	proC	3293103..3293924 (+)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
HW05_RS15170 (HW05_15295)	-	3293935..3294528 (+)	594	WP_003084544.1	YggT family protein	-
HW05_RS15175	-	3294525..3294821 (+)	297	WP_031628111.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=124962 HW05_RS15150 WP_003100959.1 3289824..3290972(-) (pilU) [Pseudomonas aeruginosa strain H47921]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=124962 HW05_RS15150 WP_003100959.1 3289824..3290972(-) (pilU) [Pseudomonas aeruginosa strain H47921]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382