Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   VAA049_RS00885 Genome accession   NZ_CP010811
Coordinates   177239..178750 (+) Length   503 a.a.
NCBI ID   WP_000138174.1    Uniprot ID   P37093
Organism   Vibrio cholerae strain 1154-74     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 172239..183750
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VAA049_RS00865 (VAA049_163) hslO 172739..173614 (-) 876 WP_046121292.1 Hsp33 family molecular chaperone HslO -
  VAA049_RS00870 (VAA049_164) hslR 173648..174025 (-) 378 WP_000098849.1 ribosome-associated heat shock protein Hsp15 -
  VAA049_RS00875 (VAA049_165) gspC 174255..175172 (+) 918 WP_042989860.1 type II secretion system protein GspC -
  VAA049_RS00880 (VAA049_166) gspD 175218..177242 (+) 2025 WP_000878557.1 type II secretion system secretin GspD -
  VAA049_RS00885 (VAA049_167) pilB/pilB1 177239..178750 (+) 1512 WP_000138174.1 type II secretion system ATPase GspE Machinery gene
  VAA049_RS00890 (VAA049_168) gspF 178750..179970 (+) 1221 WP_000718695.1 type II secretion system inner membrane protein GspF -
  VAA049_RS00895 (VAA049_169) gspG 180011..180451 (+) 441 WP_000738789.1 type II secretion system major pseudopilin GspG -
  VAA049_RS00900 (VAA049_170) gspH 180485..181063 (+) 579 WP_046121293.1 type II secretion system minor pseudopilin GspH -
  VAA049_RS00905 (VAA049_171) gspI 181053..181406 (+) 354 WP_046121294.1 type II secretion system minor pseudopilin GspI -
  VAA049_RS00910 (VAA049_172) gspJ 181393..182058 (+) 666 WP_046121295.1 type II secretion system minor pseudopilin GspJ -
  VAA049_RS00915 (VAA049_173) gspK 182048..183058 (+) 1011 WP_001201881.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 503 a.a.        Molecular weight: 56358.58 Da        Isoelectric Point: 6.2107

>NTDB_id=124374 VAA049_RS00885 WP_000138174.1 177239..178750(+) (pilB/pilB1) [Vibrio cholerae strain 1154-74]
MTEMVISPAERQSIRRLPFSFANRFKLVLDWNEDFSQASIYYLAPLSMEALVETKRVVKHAFQLIELSQAEFESKLTQVY
QRDSSEARQLMEDIGADSDDFFSLAEELPQNEDLLESEDDAPIIKLINAMLGEAIKEGASDIHIETFEKTLSIRFRVDGV
LREVLAPSRKLSSLLVSRVKVMAKLDIAEKRVPQDGRISLRIGGRAVDVRVSTMPSSHGERVVMRLLDKNATRLDLHSLG
MTAHNHDNFRRLIKRPHGIILVTGPTGSGKSTTLYAGLQELNSSERNILTVEDPIEFDIDGIGQTQVNPRVDMTFARGLR
AILRQDPDVVMVGEIRDLETAQIAVQASLTGHLVMSTLHTNTAVGAVTRLRDMGIEPFLISSSLLGVLAQRLVRTLCPDC
KEPYEADKEQRKLFDSKKKEPLILYRATGCPKCNHKGYRGRTGIHELLLVDDALQELIHSEAGEQAMEKHIRATTPSIRD
DGLDKVRQGITSLEEVMRVTKES

Nucleotide


Download         Length: 1512 bp        

>NTDB_id=124374 VAA049_RS00885 WP_000138174.1 177239..178750(+) (pilB/pilB1) [Vibrio cholerae strain 1154-74]
ATGACCGAAATGGTGATCTCTCCAGCTGAGCGACAGTCGATTCGTCGTCTGCCCTTTAGCTTCGCCAATCGCTTTAAGTT
GGTGCTGGATTGGAATGAGGATTTCTCCCAAGCCAGCATTTATTACTTAGCGCCATTGTCGATGGAGGCGCTCGTCGAAA
CCAAGCGGGTGGTCAAGCACGCTTTCCAACTGATTGAGCTCTCTCAAGCGGAGTTTGAAAGCAAGCTAACCCAAGTCTAT
CAGCGTGATTCTTCAGAAGCGCGTCAATTGATGGAAGACATTGGTGCCGACAGTGATGACTTCTTCTCACTGGCGGAAGA
GCTGCCTCAAAACGAAGATTTGCTAGAAAGTGAAGATGATGCGCCGATCATCAAATTGATCAACGCCATGTTGGGCGAAG
CGATCAAAGAAGGCGCGTCAGATATTCACATTGAAACTTTTGAAAAAACACTGTCGATCCGTTTTCGAGTCGATGGCGTG
CTGCGCGAAGTACTGGCGCCCAGCCGCAAACTCTCATCGCTGCTCGTTTCACGGGTCAAAGTGATGGCCAAGCTCGACAT
TGCCGAAAAACGAGTGCCACAAGATGGCCGTATTTCGCTGCGTATTGGTGGCCGCGCGGTCGATGTGCGGGTTTCAACCA
TGCCTTCATCACATGGTGAGCGAGTGGTAATGCGTTTGCTGGATAAAAACGCGACGCGCCTTGATCTGCACAGTTTGGGT
ATGACGGCGCATAACCATGATAACTTCCGCCGTTTGATTAAGCGGCCGCATGGGATCATTCTGGTGACTGGCCCAACCGG
CTCGGGTAAATCGACTACCTTGTACGCGGGTTTGCAAGAGCTCAACAGCAGCGAGCGCAACATTTTAACCGTAGAAGATC
CGATCGAATTTGATATTGATGGTATCGGTCAGACCCAAGTCAACCCAAGGGTAGACATGACCTTCGCACGGGGGCTACGC
GCTATTTTGCGTCAAGACCCCGATGTGGTGATGGTGGGGGAAATTCGTGACTTGGAAACGGCGCAAATTGCAGTGCAAGC
CTCGCTGACTGGTCACTTAGTGATGTCGACTCTGCACACCAACACGGCCGTTGGGGCGGTGACTCGGCTGCGCGATATGG
GCATCGAGCCTTTTTTGATCTCCTCTTCACTGCTCGGTGTTCTGGCTCAGCGCTTGGTGCGCACCTTATGCCCAGATTGC
AAAGAGCCTTACGAGGCGGACAAAGAGCAGCGCAAACTGTTTGATAGCAAGAAAAAAGAGCCGCTGATCCTTTATCGTGC
AACGGGCTGCCCTAAATGTAACCACAAAGGTTACCGTGGCCGAACCGGTATCCACGAGCTGTTGCTGGTGGATGACGCGT
TGCAGGAGCTGATCCATAGCGAGGCGGGCGAACAGGCGATGGAGAAACACATTCGCGCGACCACGCCGAGCATTCGTGAT
GATGGTTTAGATAAGGTGCGCCAAGGCATTACTTCGCTAGAAGAAGTGATGCGGGTGACTAAGGAGTCCTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(129-395)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 1P9R
  PDB 1P9W
  PDB 2BH1
  PDB 4KSR
  PDB 4KSS

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

41.412

100

0.431
  pilB Legionella pneumophila strain ERS1305867

47.126

86.481

0.408
  pilB Vibrio parahaemolyticus RIMD 2210633

44.619

88.668

0.396
  pilB Vibrio campbellii strain DS40M4

44.118

87.873

0.388
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.745

77.932

0.388
  pilB Vibrio cholerae strain A1552

48.485

78.728

0.382
  pilF Thermus thermophilus HB27

47.761

79.92

0.382
  pilB Acinetobacter baumannii D1279779

46.21

81.312

0.376