Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   ECOLIN_RS18860 Genome accession   NZ_CP007799
Coordinates   3793189..3794313 (-) Length   374 a.a.
NCBI ID   WP_000228508.1    Uniprot ID   A0A0D8VMA7
Organism   Escherichia coli Nissle 1917     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3788189..3799313
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ECOLIN_RS18830 (ECOLIN_18195) yrdA 3789989..3790543 (+) 555 WP_001286206.1 gamma carbonic anhydrase family protein -
  ECOLIN_RS18835 (ECOLIN_18200) yrdB 3790519..3790776 (-) 258 WP_001070581.1 DUF1488 domain-containing protein -
  ECOLIN_RS18840 (ECOLIN_18205) aroE 3790773..3791591 (-) 819 WP_000451246.1 shikimate dehydrogenase -
  ECOLIN_RS18845 (ECOLIN_18210) tsaC 3791596..3792168 (-) 573 WP_001296460.1 L-threonylcarbamoyladenylate synthase type 1 TsaC -
  ECOLIN_RS18850 yrdD 3792173..3792715 (-) 543 WP_001312137.1 type I DNA topoisomerase -
  ECOLIN_RS18855 (ECOLIN_18220) smg 3792744..3793217 (-) 474 WP_000460672.1 DUF494 family protein Smg -
  ECOLIN_RS18860 (ECOLIN_18225) dprA 3793189..3794313 (-) 1125 WP_000228508.1 DNA-protecting protein DprA Machinery gene
  ECOLIN_RS18865 (ECOLIN_18230) def 3794443..3794952 (+) 510 WP_000114984.1 peptide deformylase -
  ECOLIN_RS18870 (ECOLIN_18235) fmt 3794967..3795914 (+) 948 WP_000004425.1 methionyl-tRNA formyltransferase -
  ECOLIN_RS18875 (ECOLIN_18240) rsmB 3795966..3797255 (+) 1290 WP_000744773.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  ECOLIN_RS18880 (ECOLIN_18245) trkA 3797277..3798653 (+) 1377 WP_000691382.1 Trk system potassium transporter TrkA -
  ECOLIN_RS18885 (ECOLIN_18250) mscL 3798783..3799196 (+) 414 WP_000022450.1 large-conductance mechanosensitive channel protein MscL -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 40892.82 Da        Isoelectric Point: 6.0395

>NTDB_id=123357 ECOLIN_RS18860 WP_000228508.1 3793189..3794313(-) (dprA) [Escherichia coli Nissle 1917]
MVDTEIWLRLISISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGLTLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSE
FYPPQLLATTDYPGALFVEGELHALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
GVSIAVLGNGLNTIHPRRHAPLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA
LEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDE
VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=123357 ECOLIN_RS18860 WP_000228508.1 3793189..3794313(-) (dprA) [Escherichia coli Nissle 1917]
ATGGTCGATACAGAAATTTGGCTGCGTTTAATTAGCATCAGCAGCTTGTATGGCGATGATATGGTCCGTATAGCTCACTG
GCTGGCAAAACAGTCGCATATTGATGCGGTTGTATTGCAGCAAACAGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT
CATTTCCGCGAAAGAGTATCGAAAGCTCACTTTGTTGGTTGGAGCAACCCAACCATCATTTAATCCCTGCGGACAGCGAA
TTTTATCCTCCTCAACTTCTGGCGACGACAGATTACCCCGGCGCACTGTTTGTTGAAGGAGAACTGCACGCGTTGCATTC
ATTTCAGCTTGCCGTAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAGCGATGGGGACGATTATTTTGCGAAACTCTGG
CGACGCGTGGAGTGACAATTACGAGTGGACTGGCGCGTGGAATCGATGGTGTGGCGCATAAAGCGGCCTTACAGGTAAAT
GGCGTTAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGCCATGCTCCACTGGCTGCCAGTCTACT
TGAGCAAGGTGGTGCTCTCGTCTCGGAATTTCCCCTCGATGTTCCACCCCTTGCTTACAATTTCCCACGAAGAAATCGCA
TTATCAGTGGTCTAAGTAAAGGTGTACTGGTGGTGGAAGCGGCTTTGCGCAGTGGTTCGCTGGTGACAGCACGTTGTGCG
CTTGAGCAGGGGCGTGAAGTTTTTGCTTTGCCAGGTCCAATAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA
ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC
CTGAAAATTCATTTTATTCACCAGATCAGGAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG
GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCAACCTGTGCCAGAGGTAGTTACTCAACTACTCGAACTGGAGTT
AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG
TTTGA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)

DprA winged helix domain (DprA_WH)

(312-357)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D8VMA7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Vibrio campbellii strain DS40M4

48.78

98.663

0.481
  dprA Vibrio cholerae strain A1552

49.355

82.888

0.409
  dprA Glaesserella parasuis strain SC1401

44.807

90.107

0.404
  dprA Haemophilus influenzae Rd KW20

43.917

90.107

0.396
  dprA Legionella pneumophila strain ERS1305867

44.242

88.235

0.39

Multiple sequence alignment