Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   NELON_RS10820 Genome accession   NZ_CP007726
Coordinates   2186395..2188074 (-) Length   559 a.a.
NCBI ID   WP_041961550.1    Uniprot ID   -
Organism   Neisseria elongata subsp. glycolytica ATCC 29315     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2181395..2193074
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NELON_RS10795 (NELON_11325) - 2181962..2182180 (+) 219 WP_003769492.1 SlyX family protein -
  NELON_RS11910 rpmE 2182315..2182533 (+) 219 WP_081456052.1 50S ribosomal protein L31 -
  NELON_RS11915 - 2182686..2182883 (-) 198 WP_003769487.1 DNA gyrase inhibitor YacG -
  NELON_RS10800 (NELON_11330) zapD 2182880..2183641 (-) 762 WP_003769485.1 cell division protein ZapD -
  NELON_RS10805 (NELON_11335) coaE 2183656..2184258 (-) 603 WP_041961548.1 dephospho-CoA kinase -
  NELON_RS10810 (NELON_11340) pilD 2184258..2185139 (-) 882 WP_003769479.1 prepilin peptidase Machinery gene
  NELON_RS10815 (NELON_11345) pilG 2185147..2186370 (-) 1224 WP_041961549.1 type II secretion system F family protein Machinery gene
  NELON_RS10820 (NELON_11350) pilF 2186395..2188074 (-) 1680 WP_041961550.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NELON_RS10825 (NELON_11355) - 2188209..2189798 (-) 1590 WP_003769468.1 inorganic phosphate transporter -
  NELON_RS10830 (NELON_11360) - 2190232..2191533 (+) 1302 WP_003769467.1 ATP-binding protein -
  NELON_RS10835 (NELON_11365) - 2191748..2192737 (+) 990 WP_003769465.1 NAD(P)H-dependent glycerol-3-phosphate dehydrogenase -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 62260.61 Da        Isoelectric Point: 5.7232

>NTDB_id=122923 NELON_RS10820 WP_041961550.1 2186395..2188074(-) (pilF) [Neisseria elongata subsp. glycolytica ATCC 29315]
MSMGLLRVLIQNKTLSSEQAGRYQQALKDNKDIIAMLFADGITNPKALAELLAGMFNYPVLDLNYYPRSNIVQDILNEEQ
MQANACVPIFKRGNKIFLAVSDPTRIQEFQKVVFASGFQIDLVIVRHDQLMTLVEWLGQRSTTILREMSQDQLDVGASQS
LYIDNEEAEDGPIARFIHKTLSDALNAGASDIHFEFYEQMARVRFRVDGQLREVVQPPVAIRGQLASRIKVMARLDISEK
RIPQDGRIQIAFSKHGRPTDFRVSTLPTLFGEKVVMRILNSDAASLNIDQLGFEDFQKELLLEAIHRPYGMVLVTGPTGS
GKTVSLYTCLNILNTEGVNISTAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKAS
QTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCPNCKEPMERPHEHALKKAGFSDEDLAKDWTM
YRAVGCDTCRGKGYKGRAGVYEVMPITDEMQRVIMEGGTEVDIQKMAYQEGMVDLRRAGLLKVMSGITSLEEVLANTND

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=122923 NELON_RS10820 WP_041961550.1 2186395..2188074(-) (pilF) [Neisseria elongata subsp. glycolytica ATCC 29315]
ATGAGTATGGGATTATTAAGGGTTTTGATTCAGAATAAAACCCTCAGCAGTGAACAAGCTGGACGCTATCAACAAGCATT
GAAAGATAATAAAGATATTATTGCAATGTTGTTTGCTGACGGAATAACTAATCCCAAAGCATTGGCAGAATTGTTGGCCG
GGATGTTCAATTACCCTGTTCTTGATTTGAATTACTATCCGCGTAGTAATATTGTGCAGGATATTTTAAATGAGGAGCAA
ATGCAGGCTAATGCGTGTGTTCCTATATTTAAACGTGGGAATAAGATATTCCTTGCAGTGTCAGATCCAACAAGAATCCA
AGAATTCCAGAAAGTGGTATTTGCTTCAGGATTTCAAATAGATTTGGTAATTGTTCGTCATGACCAGTTAATGACGTTGG
TTGAATGGCTTGGACAGAGGTCTACCACTATCTTGCGTGAGATGTCTCAGGATCAGTTGGATGTGGGGGCATCGCAATCA
TTATATATCGATAATGAAGAGGCCGAGGATGGGCCTATTGCGCGGTTTATTCATAAAACCCTATCTGATGCATTAAATGC
AGGTGCTTCTGATATCCATTTTGAGTTTTATGAGCAGATGGCACGTGTGCGGTTCCGTGTGGATGGGCAGTTGCGAGAAG
TTGTGCAACCTCCTGTTGCAATACGTGGGCAATTGGCGTCACGTATTAAGGTGATGGCAAGATTGGATATTTCTGAAAAA
CGTATTCCTCAAGATGGACGCATCCAAATCGCTTTTTCTAAACATGGTAGACCCACAGATTTTCGTGTGAGTACATTGCC
AACGTTGTTTGGTGAAAAAGTTGTGATGCGTATTCTTAATTCGGATGCAGCATCGCTAAATATTGATCAGCTCGGGTTTG
AAGATTTTCAAAAGGAATTATTACTGGAGGCAATCCATCGGCCATATGGGATGGTTTTAGTAACTGGTCCGACAGGCTCT
GGTAAAACTGTTTCTTTGTATACTTGTCTAAACATTTTAAATACCGAAGGAGTAAATATTTCCACAGCAGAAGATCCGGC
GGAAATTAATTTGCCTGGAATTAACCAAGTAAATGTTAATGATAAACAAGGATTAACTTTTGCAGCTGCACTAAAATCGT
TTCTGCGCCAAGATCCGGATATTATTATGGTTGGTGAGATTCGTGATTTGGAAACCGCAGATATTGCCATTAAAGCATCG
CAGACAGGGCATATGGTGTTTTCTACATTGCATACCAATAATGCTCCTGCAACATTGTCTCGTATGCTGAATATGGGTGT
CGCACCATTTAATATAGCCAGTTCCGTTAGTCTCATTATGGCACAAAGGTTATTGCGTAGATTGTGCCCAAATTGTAAGG
AACCAATGGAAAGGCCTCATGAGCATGCCTTAAAAAAGGCAGGCTTTTCAGATGAAGATTTAGCTAAAGATTGGACAATG
TATCGAGCGGTAGGTTGTGATACATGCAGAGGTAAAGGTTATAAGGGGCGTGCTGGTGTATATGAAGTAATGCCAATTAC
AGATGAAATGCAAAGAGTGATTATGGAAGGTGGAACAGAAGTGGATATTCAAAAAATGGCATATCAAGAGGGAATGGTAG
ATTTACGCCGAGCAGGTTTATTGAAGGTAATGAGCGGCATTACTTCGTTAGAGGAAGTATTAGCTAATACTAATGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(179-447)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

75.671

100

0.757
  pilB Acinetobacter baumannii D1279779

51.601

100

0.519
  pilB Acinetobacter baylyi ADP1

50.626

100

0.506
  pilB Legionella pneumophila strain ERS1305867

47.95

100

0.481
  pilB Vibrio cholerae strain A1552

47.963

96.601

0.463
  pilB Vibrio parahaemolyticus RIMD 2210633

48.008

94.275

0.453
  pilB Vibrio campbellii strain DS40M4

46.49

94.275

0.438
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.313

93.739

0.369

Multiple sequence alignment