Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RR41_RS14805 Genome accession   NZ_CP007712
Coordinates   3077006..3078124 (+) Length   372 a.a.
NCBI ID   WP_000347035.1    Uniprot ID   -
Organism   Acinetobacter baumannii LAC-4     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 3054775..3101398 3077006..3078124 within 0


Gene organization within MGE regions


Location: 3054775..3101398
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RR41_RS14715 (ABLAC_29450) - 3054821..3055813 (-) 993 WP_000527288.1 FRG domain-containing protein -
  RR41_RS14720 (ABLAC_29460) - 3055928..3056383 (-) 456 WP_000549863.1 hypothetical protein -
  RR41_RS14725 (ABLAC_29470) - 3056374..3057258 (-) 885 WP_001076477.1 endonuclease/exonuclease/phosphatase family protein -
  RR41_RS14730 (ABLAC_29480) - 3057255..3058412 (-) 1158 WP_032003042.1 site-specific integrase -
  RR41_RS14735 (ABLAC_29490) ggt 3059109..3061094 (-) 1986 WP_001042937.1 gamma-glutamyltransferase -
  RR41_RS14740 (ABLAC_29500) - 3061398..3062921 (-) 1524 WP_000857089.1 DHA2 family efflux MFS transporter permease subunit -
  RR41_RS14745 (ABLAC_29510) - 3062928..3064079 (-) 1152 WP_000128703.1 EmrA/EmrK family multidrug efflux transporter periplasmic adaptor subunit -
  RR41_RS14750 (ABLAC_29520) - 3064349..3064846 (-) 498 WP_032003044.1 thioesterase family protein -
  RR41_RS14755 (ABLAC_29530) hemB 3064989..3066002 (+) 1014 WP_000222569.1 porphobilinogen synthase -
  RR41_RS14760 (ABLAC_29540) - 3066028..3067143 (+) 1116 WP_000546686.1 FAD-dependent oxidoreductase -
  RR41_RS14765 (ABLAC_29550) - 3067135..3068040 (-) 906 WP_032003045.1 TIGR01777 family oxidoreductase -
  RR41_RS14770 (ABLAC_29560) - 3068129..3068581 (-) 453 WP_000164156.1 ABZJ_00895 family protein -
  RR41_RS14775 (ABLAC_29570) - 3068961..3069338 (+) 378 WP_001091955.1 VOC family protein -
  RR41_RS14780 (ABLAC_29580) - 3069408..3069881 (-) 474 WP_000152328.1 OsmC family protein -
  RR41_RS14785 (ABLAC_29590) - 3070046..3071314 (+) 1269 WP_002037243.1 exonuclease SbcCD subunit D -
  RR41_RS14790 (ABLAC_29600) - 3071324..3074920 (+) 3597 WP_000698798.1 AAA family ATPase -
  RR41_RS14795 (ABLAC_29610) - 3075122..3075814 (-) 693 WP_001108510.1 YggS family pyridoxal phosphate-dependent enzyme -
  RR41_RS14800 (ABLAC_29620) pilT 3075941..3076978 (+) 1038 WP_000355489.1 type IV pilus twitching motility protein PilT Machinery gene
  RR41_RS14805 (ABLAC_29630) pilU 3077006..3078124 (+) 1119 WP_000347035.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RR41_RS14810 (ABLAC_29640) fur 3078194..3078631 (-) 438 WP_001122847.1 ferric iron uptake transcriptional regulator -
  RR41_RS14815 (ABLAC_29650) bamE 3078743..3079141 (+) 399 WP_001170994.1 outer membrane protein assembly factor BamE -
  RR41_RS14820 (ABLAC_29660) - 3079196..3079519 (-) 324 WP_000442585.1 RnfH family protein -
  RR41_RS14825 (ABLAC_29670) - 3079519..3080586 (-) 1068 WP_005120426.1 hypothetical protein -
  RR41_RS14830 (ABLAC_29680) - 3080739..3081194 (-) 456 WP_000782976.1 bacteriohemerythrin -
  RR41_RS14835 (ABLAC_29690) - 3081447..3082295 (-) 849 WP_000336551.1 class II glutamine amidotransferase -
  RR41_RS14840 (ABLAC_29700) - 3082404..3083186 (-) 783 WP_000890283.1 GNAT family N-acetyltransferase -
  RR41_RS14845 (ABLAC_29710) argB 3083307..3084215 (-) 909 WP_001135419.1 acetylglutamate kinase -
  RR41_RS14850 (ABLAC_29720) - 3084230..3085648 (-) 1419 WP_001102846.1 phosphomannomutase/phosphoglucomutase -
  RR41_RS14855 (ABLAC_29730) dut 3085848..3086300 (-) 453 WP_000868152.1 dUTP diphosphatase -
  RR41_RS14860 (ABLAC_29740) rep 3086325..3088364 (-) 2040 WP_000093035.1 DNA helicase Rep -
  RR41_RS14865 (ABLAC_29750) - 3088558..3089211 (-) 654 WP_001202415.1 OmpA family protein -
  RR41_RS14870 (ABLAC_29760) - 3089456..3090382 (+) 927 WP_000100976.1 acyltransferase -
  RR41_RS14875 (ABLAC_29770) - 3090398..3091444 (+) 1047 WP_032003047.1 hypothetical protein -
  RR41_RS14880 (ABLAC_29780) minC 3091566..3092288 (+) 723 WP_000763678.1 septum site-determining protein MinC -
  RR41_RS14885 (ABLAC_29790) minD 3092359..3093171 (+) 813 WP_001074362.1 septum site-determining protein MinD -
  RR41_RS14890 (ABLAC_29800) minE 3093174..3093446 (+) 273 WP_000896934.1 cell division topological specificity factor MinE -
  RR41_RS14895 (ABLAC_29810) - 3093529..3093816 (+) 288 WP_001218560.1 PA4642 family protein -
  RR41_RS14900 (ABLAC_29820) rhtC 3093859..3094479 (-) 621 WP_000959259.1 threonine export protein RhtC -
  RR41_RS14905 (ABLAC_29830) - 3094628..3097663 (-) 3036 WP_000127816.1 DNA translocase FtsK 4TM domain-containing protein -
  RR41_RS14910 (ABLAC_29840) trxB 3097933..3098880 (+) 948 WP_001276145.1 thioredoxin-disulfide reductase -
  RR41_RS14915 (ABLAC_29850) aat 3099000..3099731 (+) 732 WP_000854785.1 leucyl/phenylalanyl-tRNA--protein transferase -
  RR41_RS14920 (ABLAC_29860) - 3099750..3100565 (+) 816 WP_000844343.1 arginyltransferase -
  RR41_RS14925 (ABLAC_29870) rimI 3100610..3101065 (-) 456 WP_000621539.1 ribosomal protein S18-alanine N-acetyltransferase -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41821.98 Da        Isoelectric Point: 6.5675

>NTDB_id=122814 RR41_RS14805 WP_000347035.1 3077006..3078124(+) (pilU) [Acinetobacter baumannii LAC-4]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDSQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=122814 RR41_RS14805 WP_000347035.1 3077006..3078124(+) (pilU) [Acinetobacter baumannii LAC-4]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAGAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAATGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTATTACATTCCA
TCATGAGCGAAAAACAACGCAAAGAATTTGCAGACACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGCCAGCTTTATATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGAAAACAGCATTTAACTTTTGACAGCCAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(34-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

75.661

100

0.769
  pilU Pseudomonas stutzeri DSM 10701

68.817

100

0.688
  pilU Vibrio cholerae strain A1552

53.243

99.462

0.53
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.908

93.011

0.39

Multiple sequence alignment