Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYF   Type   Machinery gene
Locus tag   DK92_RS00675 Genome accession   NZ_CP007561
Coordinates   104995..105429 (+) Length   144 a.a.
NCBI ID   WP_002986542.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain NGAS596     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 99995..110429
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DK92_RS00645 (DK92_00665) - 101663..102028 (+) 366 WP_002986560.1 DUF1033 family protein -
  DK92_RS00650 (DK92_00670) comYA 102121..103059 (+) 939 WP_047235612.1 competence type IV pilus ATPase ComGA Machinery gene
  DK92_RS00655 (DK92_00675) comGB 102995..104030 (+) 1036 Protein_81 competence type IV pilus assembly protein ComGB -
  DK92_RS00660 (DK92_00680) comYC 104032..104358 (+) 327 WP_002986552.1 competence type IV pilus major pilin ComGC Machinery gene
  DK92_RS00665 (DK92_00685) comGD 104333..104761 (+) 429 WP_002986548.1 competence type IV pilus minor pilin ComGD -
  DK92_RS00670 (DK92_00690) comGE 104718..105002 (+) 285 WP_002987779.1 competence type IV pilus minor pilin ComGE -
  DK92_RS00675 (DK92_00695) comYF 104995..105429 (+) 435 WP_002986542.1 competence type IV pilus minor pilin ComGF Machinery gene
  DK92_RS00680 (DK92_00700) comGG 105413..105739 (+) 327 WP_010921801.1 competence type IV pilus minor pilin ComGG -
  DK92_RS00685 (DK92_00705) comYH 105837..106790 (+) 954 WP_002987790.1 class I SAM-dependent methyltransferase Machinery gene
  DK92_RS00690 (DK92_00710) - 106849..108045 (+) 1197 WP_011284424.1 acetate kinase -
  DK92_RS00695 (DK92_00715) - 108232..108540 (+) 309 WP_011284425.1 hypothetical protein -
  DK92_RS00700 (DK92_00720) proC 108623..109393 (-) 771 WP_032464773.1 pyrroline-5-carboxylate reductase -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16573.20 Da        Isoelectric Point: 10.3584

>NTDB_id=120871 DK92_RS00675 WP_002986542.1 104995..105429(+) (comYF) [Streptococcus pyogenes strain NGAS596]
MSKQLSNIKAFTLLEALIALLVISGSLLVYQGLTQTLLKRSHYLARHDQDNWLLFSHQLREELSGARFYKVADNKLYVEK
GKKVLAFGQFKSHDFRKSASNGKGYQPMLFGISRSHIHIGQSQICITLKWKSGLERTFYYAFQD

Nucleotide


Download         Length: 435 bp        

>NTDB_id=120871 DK92_RS00675 WP_002986542.1 104995..105429(+) (comYF) [Streptococcus pyogenes strain NGAS596]
TTGAGTAAACAATTAAGTAACATAAAAGCTTTTACCCTTCTAGAGGCGTTAATAGCCTTACTCGTGATATCAGGGTCTTT
ATTGGTTTATCAAGGTTTGACCCAGACCCTCCTTAAACGTAGCCATTATCTAGCCCGTCATGATCAAGACAATTGGCTCT
TATTTTCTCATCAATTGCGAGAGGAGTTAAGTGGAGCAAGATTTTACAAAGTAGCTGATAATAAACTATACGTTGAAAAG
GGAAAGAAAGTACTAGCTTTTGGCCAATTTAAAAGTCATGATTTCCGAAAATCAGCTAGTAATGGAAAAGGGTATCAACC
CATGTTATTTGGAATATCACGTAGTCATATTCACATAGGGCAGTCACAGATTTGCATTACTTTAAAGTGGAAAAGTGGGT
TAGAAAGGACTTTTTATTATGCCTTTCAAGACTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(41-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYF Streptococcus mutans UA140

51.079

96.528

0.493
  comYF Streptococcus mutans UA159

50.36

96.528

0.486
  comGF Lactococcus lactis subsp. cremoris KW2

45.985

95.139

0.437
  comGF/cglF Streptococcus mitis NCTC 12261

44.03

93.056

0.41
  comGF/cglF Streptococcus pneumoniae Rx1

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae D39

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae R6

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae TIGR4

43.609

92.361

0.403
  comGF/cglF Streptococcus mitis SK321

42.857

92.361

0.396

Multiple sequence alignment