Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   SH03_RS04280 Genome accession   NZ_CP010441
Coordinates   878308..878748 (-) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli K-12 strain K-12 MG1655     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 873308..883748
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SH03_RS04260 (SH03_04255) coaE 873798..874418 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  SH03_RS25870 - 874443..874487 (+) 45 WP_120795372.1 protein YacM -
  SH03_RS04265 (SH03_04260) guaC 874643..875686 (+) 1044 WP_001217338.1 GMP reductase -
  SH03_RS04270 (SH03_04265) hofC 875721..876923 (-) 1203 WP_000157266.1 protein transport protein HofC -
  SH03_RS04275 (SH03_04270) pilB 876913..878298 (-) 1386 WP_001025145.1 type II secretion system protein GspE Machinery gene
  SH03_RS04280 (SH03_04275) pilA 878308..878748 (-) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  SH03_RS04290 (SH03_04285) nadC 878951..879844 (-) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  SH03_RS04295 (SH03_04290) ampD 879932..880483 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  SH03_RS04300 (SH03_04295) ampE 880480..881334 (+) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  SH03_RS04305 (SH03_04300) aroP 881377..882750 (-) 1374 WP_000969915.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=120870 SH03_RS04280 WP_000360904.1 878308..878748(-) (pilA) [Escherichia coli K-12 strain K-12 MG1655]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=120870 SH03_RS04280 WP_000360904.1 878308..878748(-) (pilA) [Escherichia coli K-12 strain K-12 MG1655]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363

Multiple sequence alignment