Detailed information NTG0119161

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: F22031_RS13700
• Genome accession: NZ_CP007399
• Coordinates: 2982540..2983688 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain F22031
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2977540..2988688

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
F22031_RS13675	-	2978691..2978987 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
F22031_RS13680 (F22031_14480)	-	2978984..2979577 (-)	594	WP_003084544.1	YggT family protein	-
F22031_RS13685 (F22031_14485)	proC	2979588..2980409 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
F22031_RS13690 (F22031_14490)	-	2980421..2981113 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
F22031_RS13695 (F22031_14495)	pilT	2981328..2982362 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
F22031_RS13700 (F22031_14500)	pilU	2982540..2983688 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
F22031_RS13705 (F22031_14505)	czcD	2983696..2984595 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
F22031_RS13710 (F22031_14510)	-	2984749..2985153 (+)	405	WP_003084560.1	NINE protein	-
F22031_RS13715 (F22031_14515)	-	2985396..2986769 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
F22031_RS13720 (F22031_14520)	-	2986766..2987950 (+)	1185	WP_003117897.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=119161 F22031_RS13700 WP_003100959.1 2982540..2983688(+) (pilU) [Pseudomonas aeruginosa strain F22031]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=119161 F22031_RS13700 WP_003100959.1 2982540..2983688(+) (pilU) [Pseudomonas aeruginosa strain F22031]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382