Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   RG59_RS02280 Genome accession   NZ_CP010200
Coordinates   433261..434271 (-) Length   336 a.a.
NCBI ID   WP_000568519.1    Uniprot ID   Q32HA1
Organism   Escherichia coli strain M10     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 428261..439271
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RG59_RS02260 (RG59_02170) mepM 429228..430550 (-) 1323 WP_001184045.1 murein DD-endopeptidase MepM -
  RG59_RS02265 (RG59_02175) znuA 430566..431498 (-) 933 WP_001342995.1 zinc ABC transporter substrate-binding protein ZnuA -
  RG59_RS02270 (RG59_02180) znuC 431577..432332 (+) 756 WP_000202996.1 zinc ABC transporter ATP-binding protein ZnuC -
  RG59_RS02275 (RG59_02185) znuB 432329..433114 (+) 786 WP_000571479.1 zinc ABC transporter permease subunit ZnuB -
  RG59_RS02280 (RG59_02190) ruvB 433261..434271 (-) 1011 WP_000568519.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  RG59_RS02285 (RG59_02195) ruvA 434280..434891 (-) 612 WP_000580323.1 Holliday junction branch migration protein RuvA -
  RG59_RS02290 yobI 435030..435095 (-) 66 WP_001386853.1 stress response small protein YobI -
  RG59_RS02295 (RG59_02200) yebB 435166..435768 (+) 603 WP_001024930.1 YebB family permuted papain-like enzyme -
  RG59_RS02300 (RG59_02205) ruvC 435770..436291 (-) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  RG59_RS02305 (RG59_02210) yebC 436326..437066 (-) 741 WP_000907248.1 YebC/PmpR family DNA-binding transcriptional regulator -
  RG59_RS02310 (RG59_02215) nudB 437095..437547 (-) 453 WP_001443602.1 dihydroneopterin triphosphate diphosphatase -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37173.77 Da        Isoelectric Point: 4.7818

>NTDB_id=117767 RG59_RS02280 WP_000568519.1 433261..434271(-) (ruvB) [Escherichia coli strain M10]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TTRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=117767 RG59_RS02280 WP_000568519.1 433261..434271(-) (ruvB) [Escherichia coli strain M10]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTGGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATCTGTTGATTTTCGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAACATCGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATCTGGCAGCGATGCTCACTAACCTTGAACCGCA
TGACGTGTTGTTTATTGATGAGATCCACCGTCTTTCGCCAGTGGTGGAAGAGGTGTTGTATCCGGCAATGGAAGACTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGATCGTTTTGGTATTGTGCAGCGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGAGCTCGCGGTACGCCGCGCATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGTTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
GTTATTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACCTTGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTGCTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG
ACGACGCGGGCGTGGAATCACTTTGGCATAACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q32HA1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

53.251

96.131

0.512
  ruvB Helicobacter pylori 26695

52.038

94.94

0.494

Multiple sequence alignment