Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   AW43_RS06250 Genome accession   NZ_CP007205
Coordinates   1316443..1316877 (-) Length   144 a.a.
NCBI ID   WP_061406215.1    Uniprot ID   Q9LA12
Organism   Pasteurella multocida subsp. multocida PMTB2.1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1311443..1321877
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AW43_RS06220 (AW43_06375) - 1312007..1312276 (-) 270 WP_005722568.1 GNAT family N-acetyltransferase -
  AW43_RS06225 (AW43_06380) yacG 1312276..1312479 (-) 204 WP_005722566.1 DNA gyrase inhibitor YacG -
  AW43_RS06230 (AW43_06385) coaE 1312490..1313110 (-) 621 WP_061406213.1 dephospho-CoA kinase -
  AW43_RS06235 (AW43_06390) - 1313125..1313847 (-) 723 WP_005722562.1 prepilin peptidase -
  AW43_RS06240 (AW43_06395) - 1313847..1315058 (-) 1212 WP_005722560.1 type II secretion system F family protein -
  AW43_RS06245 (AW43_06400) pilB 1315058..1316443 (-) 1386 WP_061406214.1 GspE/PulE family protein Machinery gene
  AW43_RS06250 (AW43_06405) pilA 1316443..1316877 (-) 435 WP_061406215.1 prepilin peptidase-dependent pilin Machinery gene
  AW43_RS06255 (AW43_06415) ampD 1317037..1317603 (+) 567 WP_005719130.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  AW43_RS06260 (AW43_06420) rppH 1318189..1318785 (+) 597 WP_005722417.1 RNA pyrophosphohydrolase -
  AW43_RS06265 (AW43_06425) - 1318788..1319582 (+) 795 WP_061406216.1 sulfite exporter TauE/SafE family protein -
  AW43_RS06270 (AW43_06430) lgt 1319592..1320405 (+) 814 Protein_1200 prolipoprotein diacylglyceryl transferase -
  AW43_RS06275 (AW43_06435) - 1320402..1321253 (+) 852 WP_005722413.1 thymidylate synthase -
  AW43_RS06280 (AW43_06440) tadA 1321268..1321768 (+) 501 WP_005722410.1 tRNA adenosine(34) deaminase TadA -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15213.68 Da        Isoelectric Point: 9.1215

>NTDB_id=117713 AW43_RS06250 WP_061406215.1 1316443..1316877(-) (pilA) [Pasteurella multocida subsp. multocida PMTB2.1]
MKKAIFFSIKKGFTLIELMIVIAIIAILATIAVPSYQNYTKKAAISELLQAAAPYRAEVELCIYNTNTPSECSAGQHGIR
ANISSGKKYLSSINVKSGVITVIGQKSLEGVNYTLSATGNAVDGVSWSVKCNGGSEVFPAGFCA

Nucleotide


Download         Length: 435 bp        

>NTDB_id=117713 AW43_RS06250 WP_061406215.1 1316443..1316877(-) (pilA) [Pasteurella multocida subsp. multocida PMTB2.1]
ATGAAAAAAGCCATTTTCTTTTCGATTAAAAAAGGGTTTACGCTAATTGAATTAATGATTGTCATTGCCATTATTGCGAT
TTTAGCCACGATTGCCGTGCCATCTTATCAAAATTATACAAAAAAAGCGGCAATCTCTGAATTATTGCAAGCCGCTGCTC
CTTATCGTGCCGAGGTAGAACTTTGTATCTATAATACAAATACGCCCAGTGAATGTAGTGCAGGACAGCATGGTATTAGA
GCAAATATAAGTTCTGGTAAAAAATATCTCAGCTCAATCAATGTCAAGTCTGGCGTGATTACTGTCATTGGTCAAAAAAG
CTTAGAGGGAGTCAATTACACTTTAAGCGCGACAGGAAATGCCGTTGATGGTGTTTCATGGAGTGTAAAATGTAATGGTG
GTAGTGAAGTTTTCCCAGCAGGATTTTGCGCATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9LA12

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

59.028

100

0.59
  pilA Haemophilus influenzae Rd KW20

59.028

100

0.59
  pilA Glaesserella parasuis strain SC1401

53.02

100

0.549
  comP Acinetobacter baylyi ADP1

37.681

95.833

0.361

Multiple sequence alignment