Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   AZ30_RS08235 Genome accession   NZ_CP007176
Coordinates   1659977..1660474 (-) Length   165 a.a.
NCBI ID   WP_001789864.1    Uniprot ID   -
Organism   Staphylococcus aureus USA300-ISMMS1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1654977..1665474
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AZ30_RS08210 (AZ30_07825) gcvPB 1655139..1656611 (-) 1473 WP_000202189.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  AZ30_RS08215 (AZ30_07830) gcvPA 1656604..1657950 (-) 1347 WP_000019687.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  AZ30_RS08220 (AZ30_07835) gcvT 1657970..1659061 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  AZ30_RS08225 (AZ30_07840) - 1659220..1659744 (-) 525 WP_001015117.1 shikimate kinase -
  AZ30_RS08230 - 1659734..1659880 (-) 147 WP_001789879.1 hypothetical protein -
  AZ30_RS08235 comGF 1659977..1660474 (-) 498 WP_001789864.1 competence type IV pilus minor pilin ComGF Machinery gene
  AZ30_RS08240 (AZ30_07850) comGE 1660392..1660691 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  AZ30_RS08245 (AZ30_07855) comGD 1660678..1661124 (-) 447 WP_001791635.1 competence type IV pilus minor pilin ComGD Machinery gene
  AZ30_RS08250 (AZ30_07860) comGC 1661102..1661413 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  AZ30_RS08255 (AZ30_07865) comGB 1661427..1662497 (-) 1071 WP_000775708.1 competence type IV pilus assembly protein ComGB Machinery gene
  AZ30_RS08260 (AZ30_07870) comGA 1662469..1663443 (-) 975 WP_000697228.1 competence type IV pilus ATPase ComGA Machinery gene
  AZ30_RS08265 (AZ30_07875) - 1663495..1664118 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  AZ30_RS08270 (AZ30_07880) - 1664115..1664444 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  AZ30_RS08275 (AZ30_07885) - 1664444..1665430 (-) 987 WP_000161314.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19164.88 Da        Isoelectric Point: 10.2192

>NTDB_id=117126 AZ30_RS08235 WP_001789864.1 1659977..1660474(-) (comGF) [Staphylococcus aureus USA300-ISMMS1]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRDLTTVDFEFFSRDI
LDDFKGVDRNDIEIRQHRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=117126 AZ30_RS08235 WP_001789864.1 1659977..1660474(-) (comGF) [Staphylococcus aureus USA300-ISMMS1]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGTAAAACTTTTCTAATTGAAAGTAGGGATTTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGATGATTTTAAAGGAGTAGATAGAAACGATATTGAAATTAGGCAACACCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATTAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(67-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus MW2

100

100

1
  comGF Staphylococcus aureus N315

96.364

100

0.964

Multiple sequence alignment