Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   AZ30_RS08255 Genome accession   NZ_CP007176
Coordinates   1661427..1662497 (-) Length   356 a.a.
NCBI ID   WP_000775708.1    Uniprot ID   -
Organism   Staphylococcus aureus USA300-ISMMS1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1656427..1667497
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AZ30_RS08215 (AZ30_07830) gcvPA 1656604..1657950 (-) 1347 WP_000019687.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  AZ30_RS08220 (AZ30_07835) gcvT 1657970..1659061 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  AZ30_RS08225 (AZ30_07840) - 1659220..1659744 (-) 525 WP_001015117.1 shikimate kinase -
  AZ30_RS08230 - 1659734..1659880 (-) 147 WP_001789879.1 hypothetical protein -
  AZ30_RS08235 comGF 1659977..1660474 (-) 498 WP_001789864.1 competence type IV pilus minor pilin ComGF Machinery gene
  AZ30_RS08240 (AZ30_07850) comGE 1660392..1660691 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  AZ30_RS08245 (AZ30_07855) comGD 1660678..1661124 (-) 447 WP_001791635.1 competence type IV pilus minor pilin ComGD Machinery gene
  AZ30_RS08250 (AZ30_07860) comGC 1661102..1661413 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  AZ30_RS08255 (AZ30_07865) comGB 1661427..1662497 (-) 1071 WP_000775708.1 competence type IV pilus assembly protein ComGB Machinery gene
  AZ30_RS08260 (AZ30_07870) comGA 1662469..1663443 (-) 975 WP_000697228.1 competence type IV pilus ATPase ComGA Machinery gene
  AZ30_RS08265 (AZ30_07875) - 1663495..1664118 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  AZ30_RS08270 (AZ30_07880) - 1664115..1664444 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  AZ30_RS08275 (AZ30_07885) - 1664444..1665430 (-) 987 WP_000161314.1 ROK family glucokinase -
  AZ30_RS08280 (AZ30_07890) - 1665427..1665630 (-) 204 WP_000087561.1 YqgQ family protein -
  AZ30_RS08285 (AZ30_07895) - 1665611..1667074 (-) 1464 WP_001017867.1 rhomboid family protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 41364.77 Da        Isoelectric Point: 10.0092

>NTDB_id=117130 AZ30_RS08255 WP_000775708.1 1661427..1662497(-) (comGB) [Staphylococcus aureus USA300-ISMMS1]
MKLQCINTFKLHSKKRQLSKAQQIDLLSNLCNLLKYGFTLYQSFQFLNLQMTYKNKQLGTTILSEISNGAPCNQILSLIG
YSDTIVMQVYLAERFGNIIDVLEETVNYMKVNRKSEQRLLKTLQYPLILVSIFIAMIIILNLTVIPQFQQLYTSMNIQLS
SFQKTLSFFITSLPTIIVVMLIIVSMLAIIMKLIYNNLNMLNKINFVMKLPLISGYFQLFKTYFVTNELVLFYKNGITLQ
SIVDVYINHSSDPFRQFLGKYLLTYSEMGYGLPQILEKLKCFKPQLIKFVLQGEKRGKLEVELKLYSQILVKQIEDKAIK
QTQFLQPILFLILGLFIVAIYLVIMLPMFQMMQSIK

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=117130 AZ30_RS08255 WP_000775708.1 1661427..1662497(-) (comGB) [Staphylococcus aureus USA300-ISMMS1]
GTGAAACTACAATGCATAAATACATTTAAACTACATTCTAAGAAGCGACAATTAAGTAAGGCCCAACAAATCGACTTACT
TTCAAATTTATGTAATTTGTTGAAATATGGTTTCACTCTGTATCAAAGTTTTCAATTTTTAAATCTTCAAATGACATATA
AAAATAAGCAATTAGGTACCACCATTCTAAGTGAAATTTCAAATGGTGCACCATGCAATCAAATATTATCACTGATAGGT
TATAGCGATACTATCGTCATGCAAGTATATTTGGCAGAAAGATTTGGCAATATTATAGACGTTCTAGAAGAAACCGTAAA
TTATATGAAAGTGAATAGAAAGTCAGAACAACGATTGTTAAAGACACTGCAATACCCCTTAATACTAGTTTCTATCTTTA
TTGCTATGATTATTATATTAAACCTCACAGTAATTCCACAGTTTCAACAATTATATACTTCTATGAATATTCAACTATCT
TCTTTTCAAAAAACATTGTCTTTTTTCATTACCAGCTTACCTACTATAATTGTAGTAATGCTCATAATAGTATCTATGTT
GGCTATTATTATGAAATTAATTTATAACAATTTAAATATGCTCAATAAGATAAACTTTGTGATGAAACTACCGCTAATAT
CAGGCTATTTCCAATTATTTAAAACTTATTTTGTAACTAATGAATTAGTGTTGTTTTATAAAAATGGTATTACACTTCAA
TCAATAGTAGACGTTTATATTAACCATAGTAGTGATCCATTTAGACAGTTTCTAGGTAAATACTTATTAACTTATTCAGA
AATGGGATATGGTTTGCCTCAAATTTTAGAAAAACTAAAATGCTTTAAGCCTCAATTAATTAAGTTTGTGCTACAAGGTG
AAAAGAGAGGGAAGCTAGAAGTAGAACTAAAGTTATATTCGCAAATATTAGTAAAACAAATAGAAGATAAAGCGATAAAA
CAGACTCAGTTTTTACAGCCTATTTTATTTTTGATTTTAGGTTTATTTATTGTCGCAATTTATTTAGTAATTATGTTACC
AATGTTTCAAATGATGCAAAGTATAAAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(227-347)

Type II secretion system (T2SS), protein F (T2SSF)

(27-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Staphylococcus aureus MW2

99.719

100

0.997
  comGB Staphylococcus aureus N315

99.719

100

0.997

Multiple sequence alignment