Detailed information NTG1170273

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: ACLV7A_RS01745
• Genome accession: NZ_OZ217344
• Coordinates: 339133..340200 (+)
• Length: 355 a.a.
• NCBI ID: WP_000159551.1
• Uniprot ID: F9HLV7
• Organism: Streptococcus mitis isolate S. mitis E22
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 334133..345200

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACLV7A_RS01730 (SMIE22_03320)	amiA3	334656..336635 (+)	1980	WP_411863419.1	peptide ABC transporter substrate-binding protein	Regulator
ACLV7A_RS01735 (SMIE22_03330)	amiC	336702..338198 (+)	1497	WP_000759899.1	ABC transporter permease	Regulator
ACLV7A_RS01740 (SMIE22_03340)	amiD	338198..339124 (+)	927	WP_000103703.1	oligopeptide ABC transporter permease OppC	Regulator
ACLV7A_RS01745 (SMIE22_03350)	amiE	339133..340200 (+)	1068	WP_000159551.1	ABC transporter ATP-binding protein	Regulator
ACLV7A_RS01750 (SMIE22_03360)	amiF	340211..341137 (+)	927	WP_042900321.1	ATP-binding cassette domain-containing protein	Regulator
ACLV7A_RS01755 (SMIE22_03370)	-	341552..342430 (-)	879	WP_411863420.1	LysR family transcriptional regulator	-
ACLV7A_RS01760 (SMIE22_03380)	-	342558..342908 (-)	351	WP_000208712.1	helix-turn-helix domain-containing protein	-
ACLV7A_RS01765 (SMIE22_03390)	-	343267..344892 (+)	1626	WP_411863421.1	malolactic enzyme	-

Sequence

Protein

• Length: 355 a.a.
• Molecular weight: 39549.11 Da
• Isoelectric Point: 4.7541

>NTDB_id=1170273 ACLV7A_RS01745 WP_000159551.1 339133..340200(+) (amiE) [Streptococcus mitis isolate S. mitis E22]
MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALVGESGSGKSVLTKTFTGMLEENGRIAQGSIDYRGQDL
TALSSHKDWEQIRGAKIATIFQDPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFDEYPFQY
SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITHDLGVVASIADKVAVMYAGEIVE
YGTVEEVFYDPRHPYTWSLLSSLPQLADDKGDLYSIPGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSETHWAK
TWLLHEDAPKVEKPAVIANLHDKIREKMGFAHLED

Nucleotide

• Length: 1068 bp

>NTDB_id=1170273 ACLV7A_RS01745 WP_000159551.1 339133..340200(+) (amiE) [Streptococcus mitis isolate S. mitis E22]
ATGACAAAAGAAAAAAATGTAATTTTGACTGCTCGCGATATTGTCGTGGAATTTGACGTTCGTGACAAAGTATTAACAGC
CATTCGTGGCGTTTCCCTTGAACTAGTTGAAGGAGAAGTATTAGCCTTGGTAGGTGAGTCAGGATCAGGGAAATCTGTTT
TGACAAAGACTTTCACAGGTATGCTTGAAGAAAATGGTCGCATTGCTCAGGGTAGTATTGACTACCGTGGTCAGGATTTG
ACAGCCTTATCTTCTCACAAGGATTGGGAACAAATTCGTGGTGCTAAGATTGCGACTATCTTCCAGGACCCAATGACTAG
TTTGGACCCAATTAAAACAATTGGTAGTCAGATTACAGAAGTTATTGTAAAACACCAAGGAAAAACAGCTAAAGAAGCGA
AAGAATTGGCCATTGACTACATGAATAAGGTTGGGATTCCAGACGCAGATAGACGTTTTGATGAATACCCATTCCAATAT
TCTGGAGGAATGCGTCAACGTATTGTTATTGCTATCGCCCTTGCCTGCCGACCTGATGTCTTGATCTGTGATGAGCCAAC
AACTGCCTTGGATGTGACTATCCAAGCACAGATTATTGATTTGCTAAAATCTTTACAAAACGAGTACCATTTCACAACAA
TCTTTATCACCCACGACCTTGGTGTGGTAGCAAGTATTGCGGATAAGGTAGCGGTTATGTATGCTGGAGAAATTGTTGAG
TATGGAACTGTTGAGGAAGTCTTCTATGACCCTCGCCATCCATATACATGGAGTCTCTTGTCTAGCTTGCCTCAGCTTGC
TGATGATAAAGGGGATCTTTACTCAATCCCAGGAACACCTCCGTCACTTTATACTGACCTGAAAGGGGATGCTTTTGCCT
TGCGTTCTGACTATGCAATGCAGATTGACTTCGAACAAAAAGCTCCTCAATTCTCAGTATCAGAGACACATTGGGCTAAA
ACTTGGCTTCTTCATGAGGATGCTCCAAAAGTAGAAAAACCAGCTGTGATTGCAAATCTCCACGATAAGATCCGTGAAAA
AATGGGATTTGCCCATCTGGAAGACTAG

Domains

Predicted by InterproScan.

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (239-301)

• ABC transporter (ABC_tran) (29-187)

3D structure

• AlphaFold DB: F9HLV7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	87.887	100	0.879
amiE	Streptococcus thermophilus LMG 18311	86.197	100	0.862
amiE	Streptococcus thermophilus LMD-9	86.197	100	0.862
oppD	Streptococcus mutans UA159	54.261	99.155	0.538