Detailed information NTG1170110

Bioinformatically predicted

Overview

• Name: cclA/cilC
• Type: Machinery gene
• Locus tag: ACLV65_RS02345
• Genome accession: NZ_OZ217342
• Coordinates: 449735..450394 (-)
• Length: 219 a.a.
• NCBI ID: WP_000565023.1
• Uniprot ID: A0A0I8D2P0
• Organism: Streptococcus mitis isolate S. mitis B22
• Function: processing and translocation of ComGC; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	451138..460216	449735..450394	flank	744

Gene organization within MGE regions

Location: 449735..460216

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACLV65_RS02345 (SMIB22_04520)	cclA/cilC	449735..450394 (-)	660	WP_000565023.1	prepilin peptidase	Machinery gene
ACLV65_RS02350 (SMIB22_04530)	-	450464..450910 (+)	447	WP_000155406.1	GNAT family N-acetyltransferase	-
ACLV65_RS02355 (SMIB22_04540)	-	451138..452316 (-)	1179	WP_000175880.1	ROK family transcriptional regulator	-
ACLV65_RS02360 (SMIB22_04550)	-	452596..453858 (+)	1263	WP_000413336.1	PTS sugar transporter subunit IIC	-
ACLV65_RS02365 (SMIB22_04560)	-	453861..454757 (+)	897	WP_000825669.1	PEP phosphonomutase	-
ACLV65_RS02370 (SMIB22_04570)	-	454772..455089 (+)	318	WP_001151646.1	PTS sugar transporter subunit IIB	-
ACLV65_RS02375 (SMIB22_04580)	-	455106..455423 (+)	318	WP_001005881.1	PTS lactose/cellobiose transporter subunit IIA	-
ACLV65_RS02380 (SMIB22_04590)	-	455433..456788 (+)	1356	WP_411864421.1	PTS sugar transporter subunit IIC	-
ACLV65_RS02385 (SMIB22_04600)	-	457111..458586 (+)	1476	WP_411864422.1	arylsulfatase	-
ACLV65_RS02390 (SMIB22_04610)	-	458583..459431 (+)	849	WP_411864423.1	formylglycine-generating enzyme family protein	-
ACLV65_RS02395 (SMIB22_04620)	-	459446..460216 (+)	771	WP_411864424.1	sulfite exporter TauE/SafE family protein	-

Sequence

Protein

• Length: 219 a.a.
• Molecular weight: 24921.13 Da
• Isoelectric Point: 8.2409

>NTDB_id=1170110 ACLV65_RS02345 WP_000565023.1 449735..450394(-) (cclA/cilC) [Streptococcus mitis isolate S. mitis B22]
MIDFYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTRLRPLDLIPILSQVFNRFRCRYCKVRYPVWYALFELVLG
LLFLLYSWKLLSLGQVVLITAGLTLGIYDFHHQEYPLLVWMTFHLILIASSGWNLVMVSFLILGILAHFIDIRMGAGDFL
FLASCALVFSVTELLILIQFASATGILAFLLQKKKERLPFVPFLLLATCLIIFGKLLLV

Nucleotide

• Length: 660 bp

>NTDB_id=1170110 ACLV65_RS02345 WP_000565023.1 449735..450394(-) (cclA/cilC) [Streptococcus mitis isolate S. mitis B22]
ATGATTGATTTTTATTTTTTTCTCGTCGGGAGCATTCTCGCTTCCTTTCTTGGTTTGGTCATTGACCGTTTTCCAGAGCA
ATCCATTATCAGTTCAGCCAGTCACTGCGATTCCTGTCAGACTCGCTTGCGTCCCTTAGATTTGATTCCGATTCTCTCAC
AGGTCTTCAATCGCTTTCGCTGTCGCTACTGCAAAGTTCGCTATCCTGTCTGGTATGCCCTCTTTGAATTAGTCTTAGGA
CTCCTCTTTCTGCTTTACTCTTGGAAATTGCTTTCCTTGGGGCAAGTAGTCCTAATCACCGCTGGTTTGACCTTGGGTAT
CTACGACTTTCACCATCAGGAATATCCCTTACTGGTCTGGATGACTTTCCACCTAATCCTAATAGCTTCCTCTGGCTGGA
ATCTGGTCATGGTCTCCTTCCTCATACTTGGAATTTTGGCTCATTTTATCGATATCCGCATGGGCGCAGGGGATTTCCTC
TTTTTAGCTTCTTGTGCTCTCGTCTTTAGCGTAACGGAGTTACTGATCTTGATTCAGTTTGCTTCTGCGACGGGTATCCT
GGCCTTTCTCCTGCAAAAGAAAAAGGAAAGACTTCCTTTCGTGCCTTTCCTCTTACTTGCTACTTGTTTGATTATTTTTG
GTAAGCTACTGCTTGTTTGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (8-89)

3D structure

• AlphaFold DB: A0A0I8D2P0

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
cclA/cilC	Streptococcus pneumoniae TIGR4	97.717	100	0.977
cclA/cilC	Streptococcus pneumoniae Rx1	96.347	100	0.963
cclA/cilC	Streptococcus pneumoniae D39	96.347	100	0.963
cclA/cilC	Streptococcus pneumoniae R6	96.347	100	0.963
cclA/cilC	Streptococcus mitis SK321	92.237	100	0.922
cclA/cilC	Streptococcus mitis NCTC 12261	86.301	100	0.863