Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   ACLV7Q_RS10080 Genome accession   NZ_OZ217341
Coordinates   2109595..2110893 (-) Length   432 a.a.
NCBI ID   WP_218757916.1    Uniprot ID   -
Organism   Streptococcus mitis isolate S. mitis C22     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2104595..2115893
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLV7Q_RS10060 (SMIC22_19910) rplI 2105750..2106202 (-) 453 WP_000864214.1 50S ribosomal protein L9 -
  ACLV7Q_RS10065 (SMIC22_19920) - 2106199..2108172 (-) 1974 WP_175880402.1 DHH family phosphoesterase -
  ACLV7Q_RS10070 (SMIC22_19930) hpf 2108308..2108856 (-) 549 WP_050142688.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  ACLV7Q_RS10075 (SMIC22_19940) comFC/cflB 2108936..2109598 (-) 663 WP_050224209.1 ComF family protein Machinery gene
  ACLV7Q_RS10080 (SMIC22_19950) comFA/cflA 2109595..2110893 (-) 1299 WP_218757916.1 DEAD/DEAH box helicase Machinery gene
  ACLV7Q_RS10085 (SMIC22_19960) - 2110949..2111584 (+) 636 WP_050242837.1 YigZ family protein -
  ACLV7Q_RS10090 - 2111681..2111773 (+) 93 Protein_1946 PH domain-containing protein -
  ACLV7Q_RS10095 (SMIC22_19970) cysK 2111870..2112796 (+) 927 WP_000029881.1 cysteine synthase A -
  ACLV7Q_RS10100 (SMIC22_19980) tsf 2112918..2113958 (-) 1041 WP_050391223.1 translation elongation factor Ts -
  ACLV7Q_RS10105 (SMIC22_19990) rpsB 2114038..2114817 (-) 780 WP_218757915.1 30S ribosomal protein S2 -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 49614.38 Da        Isoelectric Point: 7.8671

>NTDB_id=1170073 ACLV7Q_RS10080 WP_218757916.1 2109595..2110893(-) (comFA/cflA) [Streptococcus mitis isolate S. mitis C22]
MKVNPNYLGHLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCNSAILDEWYLPIGAYYCRECLLMKRVRSDQALYYFPQ
EDFPKQDVLKWRGQLTPFQEKVSEGLIQAVEKQEPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
LQDDFACEISLLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPTLYHAVKNSVKENGLRIFLTATST
DELDKKVRLGELKRLSLPRRFHGNPLIIPKSIWLSDFNRYLDRNRLSPKLKTYIEKQRKTAYPLLIFASEIKKGEQLKEI
LQEQFPNEKIGFVSSITEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGGRVGRSMDR
PTGDLLFFHDGLNDSIKKAIKEIKQMNKEAGL

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=1170073 ACLV7Q_RS10080 WP_218757916.1 2109595..2110893(-) (comFA/cflA) [Streptococcus mitis isolate S. mitis C22]
ATGAAAGTAAATCCAAATTATCTCGGTCATTTGTTTACGGAGAATGAATTAACAGAAGAAGAACGTCAGTTGGCGGAGAA
ACTTCCAGCAATGAGAAAGGAGAAAGGGAAACTTTTCTGTCAACGTTGTAATAGTGCTATTTTAGATGAATGGTATTTGC
CCATCGGTGCTTACTATTGTAGGGAGTGTTTACTGATGAAGCGAGTCAGGAGTGATCAAGCTTTATACTATTTTCCACAG
GAGGATTTTCCGAAGCAAGATGTTCTTAAATGGCGTGGTCAATTAACTCCTTTTCAAGAGAAGGTGTCAGAGGGACTGAT
TCAAGCAGTAGAGAAGCAAGAGCCAACTTTAGTTCATGCGGTAACAGGAGCAGGAAAGACAGAAATGATTTATCAAGTTG
TGGCTAAAGTAATCAATGCGGGTGGTGCAGTGTGTTTGGCCAGTCCTCGCATAGATGTTTGTTTGGAGCTGTACAAGCGC
CTGCAAGATGATTTTGCTTGCGAGATATCTTTACTACACGGAGAATCAGAGCCGTATTTTCGAACACCACTAGTTGTTGC
GACGACCCATCAGTTATTGAAGTTTTATCAAGCTTTTGATTTGCTGATAGTGGATGAAGTAGATGCTTTTCCTTATGTTG
ACAATCCCACGCTTTACCACGCTGTCAAGAATAGTGTAAAGGAGAATGGATTGAGAATCTTTTTAACAGCGACTTCGACC
GATGAGTTAGATAAAAAGGTCCGTTTAGGAGAACTAAAAAGACTGAGCTTACCGAGACGATTTCATGGAAATCCGTTGAT
TATTCCAAAATCAATTTGGTTATCGGATTTTAATCGCTACTTAGATAGGAATCGTTTGTCACCAAAGTTAAAGACTTATA
TTGAGAAACAGAGAAAGACAGCTTATCCGTTACTCATTTTTGCTTCAGAAATTAAGAAAGGGGAGCAGTTAAAAGAAATC
TTACAGGAGCAATTTCCAAATGAGAAAATTGGATTTGTCTCTTCTATCACAGAAGATCGATTAGAGCAGGTACAAGCTTT
TCGAGATGGAGAACTGACAATACTTATCAGTACGACAATCTTGGAGCGTGGGGTTACCTTCCCTTGTGTGGACGTTTTCG
TAGTAGAGGCTAATCATCGTCTGTTTACCAAGTCTAGTTTGATTCAGATTGGTGGACGAGTTGGACGAAGCATGGATAGA
CCGACAGGAGATTTGCTTTTCTTCCATGATGGATTAAATGATTCAATCAAGAAGGCGATTAAGGAAATTAAGCAGATGAA
CAAGGAGGCTGGTCTATGA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(294-396)

Type III restriction enzyme, res subunit (ResIII)

(94-238)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

94.444

100

0.944
  comFA/cflA Streptococcus pneumoniae D39

94.444

100

0.944
  comFA/cflA Streptococcus pneumoniae R6

94.444

100

0.944
  comFA/cflA Streptococcus pneumoniae TIGR4

94.213

100

0.942
  comFA/cflA Streptococcus mitis SK321

93.75

100

0.938
  comFA/cflA Streptococcus mitis NCTC 12261

93.287

100

0.933
  comFA Lactococcus lactis subsp. cremoris KW2

50.816

99.306

0.505
  comFA Latilactobacillus sakei subsp. sakei 23K

37.931

100

0.382
  comFA Bacillus subtilis subsp. subtilis str. 168

38.806

93.056

0.361

Multiple sequence alignment