Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ACH8EH_RS08600 Genome accession   NZ_OZ197914
Coordinates   1637904..1639580 (-) Length   558 a.a.
NCBI ID   WP_003689817.1    Uniprot ID   A0AAQ1E824
Organism   Neisseria gonorrhoeae isolate SGC-24-002     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1632904..1644580
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACH8EH_RS08580 pilG 1634821..1636053 (+) 1233 WP_003689811.1 type 4 pilus assembly protein PilG Machinery gene
  ACH8EH_RS08585 pilD 1636127..1636987 (+) 861 WP_003689814.1 A24 family peptidase Machinery gene
  ACH8EH_RS08590 coaE 1636984..1637616 (+) 633 WP_003689816.1 dephospho-CoA kinase -
  ACH8EH_RS08595 - 1637609..1637818 (+) 210 WP_003696096.1 DNA gyrase inhibitor YacG -
  ACH8EH_RS08600 pilF 1637904..1639580 (-) 1677 WP_003689817.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACH8EH_RS08610 - 1639918..1640364 (-) 447 WP_003689818.1 DUF441 domain-containing protein -
  ACH8EH_RS08615 - 1640368..1641342 (-) 975 WP_003689819.1 polyprenyl synthetase family protein -
  ACH8EH_RS08620 rplU 1641566..1641874 (+) 309 WP_003691838.1 50S ribosomal protein L21 -
  ACH8EH_RS08625 rpmA 1641899..1642171 (+) 273 WP_003689821.1 50S ribosomal protein L27 -
  ACH8EH_RS08630 ubiM 1642406..1643590 (+) 1185 WP_020996717.1 5-demethoxyubiquinol-8 5-hydroxylase UbiM -
  ACH8EH_RS08635 - 1643602..1643730 (+) 129 WP_258028158.1 hypothetical protein -
  ACH8EH_RS08640 rpmG 1643779..1643934 (-) 156 WP_003689824.1 50S ribosomal protein L33 -
  ACH8EH_RS08645 rpmB 1643966..1644199 (-) 234 WP_002216391.1 50S ribosomal protein L28 -

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 61863.00 Da        Isoelectric Point: 5.5420

>NTDB_id=1169775 ACH8EH_RS08600 WP_003689817.1 1637904..1639580(-) (pilF) [Neisseria gonorrhoeae isolate SGC-24-002]
MSVGLLRILVQNQVVTVEQAEHYYNESQAGKEVLPMLFSDGVISPKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQ
MVEFHCVPVFRRGDKVFFAVSDPTQMPQIQKTVSAAGIAVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTL
YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPIAVRGQLASRIKVMSRLDISEKR
IPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMRILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSG
KTVSLYTCLNILNTESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKAAQ
TGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSSCKQEVERPSASALKEVGFTDEDLAKDWKLY
RAVGCDRCRGQGYKGRAGVYEVMPISEEMQRVIMNNGTEVGILDVAYKEGMVDLRRAGILKIMQGITSLEEVTANTND

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=1169775 ACH8EH_RS08600 WP_003689817.1 1637904..1639580(-) (pilF) [Neisseria gonorrhoeae isolate SGC-24-002]
ATGAGCGTAGGTTTGCTGAGGATTCTGGTTCAAAACCAGGTGGTTACTGTTGAGCAGGCCGAGCATTACTACAATGAGTC
GCAGGCGGGTAAGGAAGTGTTGCCGATGCTGTTTTCAGACGGTGTCATTTCGCCCAAGTCGCTTGCGGCATTGATTGCGA
GGGTGTTCAGTTATTCGATTCTTGATTTGCGTCATTATCCGCGCCACAGGGTGCTGATGGGGGTGTTGACGGAGGAGCAG
ATGGTGGAGTTCCACTGTGTGCCGGTTTTCCGTCGGGGCGACAAAGTATTTTTTGCGGTTTCCGATCCGACCCAGATGCC
GCAAATTCAGAAAACCGTTTCTGCCGCAGGGATTGCGGTTGAGTTGGTCATTGTCGAGGATGACCAGTTGGCGGGTTTGC
TCGATTGGGTGGGTTCGCGTTCGACATCGCTGCTTCAGGAGCTTGGGGAGGGGCAAGAGGAAGAGGAAAGCCACACCCTG
TATATCGACAACGAGGAGGCAGAAGACGGCCCTGTTCCGAGGTTTATCCATAAAACTTTGTCGGATGCCTTGCGTAGCGG
GGCATCCGACATCCATTTCGAGTTTTACGAACACAATGCGCGTATCCGTTTCCGTGTGGACGGGCAGCTCCGCGAGGTGG
TTCAGCCGCCCATTGCGGTAAGGGGGCAGCTTGCTTCCCGGATTAAGGTAATGTCGCGTTTGGACATTTCCGAAAAACGG
ATACCGCAGGACGGTAGGATGCAGCTGACCTTTCAAAAGGGCGGCAAGCCTGTCGATTTCCGTGTCAGCACATTGCCGAC
GCTGTTTGGCGAAAAGGTCGTGATGCGGATTTTGAATTCCGATGCCGCGTCTTTGAACATCGACCAGCTCGGTTTTGAGC
CGTTCCAGAAAAAATTGTTGTTGGAAGCGATTCACCGTCCTTACGGGATGGTGCTGGTAACCGGCCCGACGGGTTCGGGT
AAGACGGTGTCGCTCTATACCTGTTTGAATATTTTGAATACGGAGTCGGTAAATATTGCAACGGCGGAAGACCCTGCCGA
GATTAACCTGCCGGGCATCAATCAGGTTAACGTCAATGATAAGCAGGGTCTGACTTTTGCCGCTGCTTTGAAGTCTTTCC
TGCGTCAGGACCCGGACATCATTATGGTCGGTGAGATTCGTGATTTGGAAACTGCCGATATTGCGATTAAGGCGGCACAA
ACAGGGCATATGGTGTTTTCCACACTGCACACGAATAATGCGCCGGCGACGTTGTCGCGTATGCTGAATATGGGTGTCGC
GCCGTTTAATATTGCCAGTTCGGTCAGCCTGATTATGGCGCAGCGTCTTTTACGCAGGCTGTGTTCGAGCTGCAAACAGG
AAGTGGAACGCCCGTCTGCCTCTGCTTTGAAGGAAGTCGGTTTCACCGATGAGGATCTTGCAAAAGATTGGAAACTTTAC
CGCGCCGTCGGTTGCGACCGTTGCCGGGGGCAGGGTTATAAGGGGCGTGCGGGCGTGTATGAGGTTATGCCCATCAGCGA
AGAAATGCAGCGTGTGATTATGAACAACGGTACGGAAGTGGGTATTTTGGACGTTGCCTATAAGGAGGGTATGGTGGATT
TGCGCCGGGCCGGTATTTTGAAAATTATGCAGGGCATTACTTCATTGGAAGAGGTAACGGCAAATACCAACGATTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-139)

Type II/IV secretion system protein (T2SSE)

(178-446)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

100

100

1
  pilB Acinetobacter baumannii D1279779

50.088

100

0.509
  pilB Acinetobacter baylyi ADP1

50.178

100

0.505
  pilB Legionella pneumophila strain ERS1305867

47.173

100

0.478
  pilB Vibrio parahaemolyticus RIMD 2210633

45.567

100

0.461
  pilB Vibrio cholerae strain A1552

45.374

100

0.457
  pilB Vibrio campbellii strain DS40M4

43.838

100

0.446
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.885

93.907

0.375

Multiple sequence alignment