Detailed information NTG1169453

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACNUEH_RS08345
• Genome accession: NZ_OZ197095
• Coordinates: 1735219..1736337 (-)
• Length: 372 a.a.
• NCBI ID: WP_057065828.1
• Uniprot ID: -
• Organism: Acinetobacter baumannii isolate 23S01404-6
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1730219..1741337

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACNUEH_RS08315	-	1731048..1731896 (+)	849	WP_000336551.1	class II glutamine amidotransferase	-
ACNUEH_RS08320	-	1732149..1732604 (+)	456	WP_000782976.1	bacteriohemerythrin	-
ACNUEH_RS08325	-	1732757..1733824 (+)	1068	WP_057065834.1	hypothetical protein	-
ACNUEH_RS08330	-	1733824..1734147 (+)	324	WP_000442585.1	RnfH family protein	-
ACNUEH_RS08335	-	1734202..1734600 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
ACNUEH_RS08340	fur	1734712..1735149 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
ACNUEH_RS08345	pilU	1735219..1736337 (-)	1119	WP_057065828.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACNUEH_RS08350	pilT	1736365..1737402 (-)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
ACNUEH_RS08355	-	1737529..1738221 (+)	693	WP_032043501.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41887.08 Da
• Isoelectric Point: 6.6944

>NTDB_id=1169453 ACNUEH_RS08345 WP_057065828.1 1735219..1736337(-) (pilU) [Acinetobacter baumannii isolate 23S01404-6]
MDFNDLLNLMVEKKSSDLFITDGVAPSIKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=1169453 ACNUEH_RS08345 WP_057065828.1 1735219..1736337(-) (pilU) [Acinetobacter baumannii isolate 23S01404-6]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATAAAGATTAATGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCA
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCACCAGATGTCATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTTGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCACAGCCAGCTTTACATGGATTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (12-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.022	98.925	0.683
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392