Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   ACFEL6_RS16930 Genome accession   NZ_OZ183475
Coordinates   2278064..2278645 (-) Length   193 a.a.
NCBI ID   WP_375321285.1    Uniprot ID   -
Organism   Aliivibrio logei isolate 622f83115ee5131b54a8b4d1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2273064..2283645
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFEL6_RS16910 aroB 2273924..2275012 (-) 1089 WP_375321282.1 3-dehydroquinate synthase -
  ACFEL6_RS16915 aroK 2275051..2275569 (-) 519 WP_012551169.1 shikimate kinase AroK -
  ACFEL6_RS16920 pilQ 2275794..2277527 (-) 1734 WP_375321283.1 type IV pilus secretin PilQ Machinery gene
  ACFEL6_RS16925 pilP 2277553..2278071 (-) 519 WP_375321284.1 pilus assembly protein PilP Machinery gene
  ACFEL6_RS16930 pilO 2278064..2278645 (-) 582 WP_375321285.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACFEL6_RS16935 - 2278642..2279208 (-) 567 WP_375321286.1 PilN domain-containing protein -
  ACFEL6_RS16940 pilM 2279193..2280191 (-) 999 WP_375321287.1 type IV pilus biogenesis protein PilM -
  ACFEL6_RS16945 - 2280330..2282828 (+) 2499 WP_375321288.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 193 a.a.        Molecular weight: 22791.21 Da        Isoelectric Point: 4.5883

>NTDB_id=1168056 ACFEL6_RS16930 WP_375321285.1 2278064..2278645(-) (pilO) [Aliivibrio logei isolate 622f83115ee5131b54a8b4d1]
MTDWRELDLDEITEWPLIPQLLMILLLCVFVQAAGYWFWLQPIMEDTEQLKRDEISLKTTVKYKYNQVAAMPEMENQLNE
LNLRYEFLAQQLPAQKELASMLSGINQVGIQNKLTFTRIDWGEKQNQEFLYKLPLNIELTGQYHDIGLFSEAIADLPRIV
SLENIDIQRVSRESSTLHFRVMAYTYQFRGKHE

Nucleotide


Download         Length: 582 bp        

>NTDB_id=1168056 ACFEL6_RS16930 WP_375321285.1 2278064..2278645(-) (pilO) [Aliivibrio logei isolate 622f83115ee5131b54a8b4d1]
ATGACAGATTGGCGAGAGTTAGATCTCGATGAAATAACAGAATGGCCATTGATACCACAACTGTTAATGATTTTATTACT
TTGTGTTTTTGTACAAGCAGCAGGATATTGGTTTTGGTTGCAACCAATAATGGAAGACACAGAGCAATTAAAGAGAGATG
AAATTAGCTTAAAAACGACGGTGAAGTATAAATACAACCAAGTTGCAGCGATGCCAGAAATGGAAAATCAGTTGAATGAA
CTCAATTTACGTTATGAGTTTTTAGCCCAGCAATTACCTGCGCAAAAAGAATTAGCAAGTATGCTTTCTGGCATTAATCA
GGTTGGTATTCAAAATAAATTAACCTTCACCCGAATCGATTGGGGGGAAAAACAGAATCAGGAATTTCTTTATAAGTTAC
CGCTAAATATTGAATTAACGGGCCAATACCATGATATCGGTTTGTTTTCAGAAGCGATTGCTGATCTGCCTCGTATTGTT
AGCTTGGAAAATATAGATATTCAACGAGTGAGTAGAGAGAGCAGTACCTTACACTTTCGTGTCATGGCTTATACCTACCA
GTTTAGGGGGAAACATGAGTAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(48-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

60.847

97.927

0.596
  pilO Vibrio campbellii strain DS40M4

58.73

97.927

0.575

Multiple sequence alignment